THE NASCENT CODE

Unravelling the Nascent Code: Signals sensed and transmitted by the nascent chain to acquire its functional state or reprogram gene expression

Coordinatore	ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOCIENCIAS    more  Organization address address: PARQUE TECNOLOGICO EDIFICIO 801 A city: DERIO VIZCAYA postcode: 48160 contact info Titolo: Mr. Nome: Alfonso Cognome: Egaña Email: send email Telefono: +34 944061300 Fax: +34 946572502
Nazionalità Coordinatore	Spain [ES]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-CIG
Funding Scheme	MC-CIG
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-07-14   -   2018-07-13

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOCIENCIAS  Organization address address: PARQUE TECNOLOGICO EDIFICIO 801 A city: DERIO VIZCAYA postcode: 48160 contact info Titolo: Mr. Nome: Alfonso Cognome: Egaña Email: send email Telefono: +34 944061300 Fax: +34 946572502	ES (DERIO VIZCAYA)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'Emerging biochemical and biophysical data strongly suggest that the transformation of the potential set of proteins, encrypted in the genome, into its actual vital functional set, is a manifestation not just of the genetic code, but of an additional layer of information encoded within the newly synthesized polypeptide nascent chain. To date, we know that immediately upon the first steps of protein synthesis, a variety of signals are formed within the evolving nascent chain (NC). These signals, by acting directly on the ribosome or by recruiting additional cellular machines, have the ability to direct new steps in the transformation of the NC and guide it towards the acquisition of its final functional state and cellular location. Due to its distinct nature, I will refer this ensemble of signals as THE NASCENT CODE. Despite our knowledge of its existence, we know very little about its mechanism of action. The goal of this research is to unravel the molecular determinants of the nascent code and to decipher how the signals, embedded in the primary sequence of the NC, orchestrate the concerted action of the ribosome and its ancillary factors (i,e, SecA, TF). The approach entails the reconstructing of the process describing the birth of a protein and following its progress over time. The techniques used in the analysis of this process, namely fluorescence spectroscopy, Cryo electron microscopy, solid state NMR-DNP, solution state NMR and X-ray crystallography, will illuminate macroscopic to atomic level details of the dynamics, structure and interactions of the key players involved. The realization of this research project will allow us to understand an essential process in the cell, for which very little structural information is currently available, and provide the structural foundation to intervene and control this process for biotechnological or therapeutic reasons.'