SMDNA

Novel approaches to the study of enzymatic diffusion on single DNA molecules

 Coordinatore KATHOLIEKE UNIVERSITEIT LEUVEN 

 Organization address address: Oude Markt 13
city: LEUVEN
postcode: 3000

contact info
Titolo: Dr.
Nome: Stijn
Cognome: Delauré
Email: send email
Telefono: 3216320944
Fax: 3216324198

 Nazionalità Coordinatore Belgium [BE]
 Totale costo 0 €
 EC contributo 167˙154 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-IEF-2008
 Funding Scheme MC-IEF
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-05-01   -   2011-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    KATHOLIEKE UNIVERSITEIT LEUVEN

 Organization address address: Oude Markt 13
city: LEUVEN
postcode: 3000

contact info
Titolo: Dr.
Nome: Stijn
Cognome: Delauré
Email: send email
Telefono: 3216320944
Fax: 3216324198

BE (LEUVEN) coordinator 167˙154.64

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

bind    molecules    fellow    molecule    single    fellowship    techniques    biophysical    first    compacted    diffusion    fluorescence    sequence    move    enzymes    dna    cell    enzymatic   

 Obiettivo del progetto (Objective)

'In cells, enzymes are able to locate and sequence specifically bind to short regions of DNA in a compacted genome of many millions of bases. This fellowship will investigate the mechanism by which they achieve this remarkable feat by examining the behavior of single enzymes diffusing on single DNA molecules. The fellowship will focus on two main objectives. The first is to exploit novel DNA-labeling technology to investigate, for the first time at the single molecule level, the effect of DNA topology on the diffusion of enzymes. Since genetic DNA is highly compacted and adopts plectonemic structures, the study of enzymatic diffusion on supercoiled DNA molecules is critical to our understanding of how enzymes interact with DNA in the cell. The second objective is to investigate the relationship between enzymatic structure and the method used by an enzyme to move on the DNA duplex. This will be done by directly visualizing structurally well-characterized enzymes as they move on a single DNA molecule. The fellow brings a background expertise of fluorescence-based techniques and molecular biology to the fellowship. The fellowship aims to train the fellow in the application of single molecule imaging to the biophysical systems that he has studied in the past. Such knowledge will significantly enhance the ability of the fellow to initiate novel research in the area of biophysical systems in the future.'

Introduzione (Teaser)

A European initiative adopted fluorescence-based techniques to visualise how enzymes find their way within a cell to bind a specific DNA sequence.

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