LARGEMS

The Dynamic Composition of Protein Complexes: A New Perspective in Structural Biology

 Coordinatore WEIZMANN INSTITUTE OF SCIENCE 

Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.

 Nazionalità Coordinatore Israel [IL]
 Totale costo 1˙500˙000 €
 EC contributo 1˙500˙000 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2009-StG
 Funding Scheme ERC-SG
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-09-01   -   2014-08-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    WEIZMANN INSTITUTE OF SCIENCE

 Organization address address: HERZL STREET 234
city: REHOVOT
postcode: 7610001

contact info
Titolo: Dr.
Nome: Michal
Cognome: Sharon
Email: send email
Telefono: -3917
Fax: -5980

IL (REHOVOT) hostInstitution 1˙500˙000.00
2    WEIZMANN INSTITUTE OF SCIENCE

 Organization address address: HERZL STREET 234
city: REHOVOT
postcode: 7610001

contact info
Titolo: Ms.
Nome: Gabi
Cognome: Bernstein
Email: send email
Telefono: +972 8 934 6728
Fax: +972 8 934 4165

IL (REHOVOT) hostInstitution 1˙500˙000.00

Mappa


 Word cloud

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cellular    organization    progression    revealing    protein    exists    complexes    cell    structural    dynamic    composition    function    cycle   

 Obiettivo del progetto (Objective)

'80% of the proteome exists in complexes or large macromolecular assemblies. It is accepted that revealing the structure of these protein complexes is a key towards mechanistic understanding of cellular processes. Yet, this might not be sufficient; a higher level of complexity probably exists and protein complexes may not be static and uniform in form and function as thought. A protein complex may actually represent an ensemble of compositionally distinct entities with functional versatility. My main aim is to provide evidence for this conceptual change and to reveal the dynamic architecture of a protein assembly. As a model system, I will investigate the COP9 signalosome (CSN), an evolutionary conserved multisubunit complex, which is involved in a variety of essential functions ranging from cell-cycle progression, DNA-repair and apoptosis. My strategy is based on a comprehensive approach, made up of four main steps; i) Revealing the structural organization of the native complex. ii) Establishing whether the complex has co-existing independent modules that function separately of, or coordinately with the holocomplex. iii) Monitoring in real-time the biogenesis and activation pathway of the complex and developing an approach for shifting its oligomerization equilibrium. iv) Determining the correlation between modularity of the complex and cell cycle progression and comparing its composition in healthy versus cancerous cells. I will integrate genetic, biochemical and structural biology approaches. In particular, I will apply a state of the art mass spectrometry technique, that will enable us to define the stoichiometry, subunit composition, dynamic interactions and structural organization of protein complexes isolated directly from the cellular environment.'

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