DIB SCREENING

Rapid functional characterization of ion channels with droplet interface bilayers

Coordinatore	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD    more  Organization address address: University Offices, Wellington Square city: OXFORD postcode: OX1 2JD contact info Titolo: Ms. Nome: Linda Cognome: Pialek Email: send email Telefono: +44 1865 289811 Fax: +44 1865 289801
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	180˙783 €
EC contributo	180˙783 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-IEF-2008
Funding Scheme	MC-IEF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-06-01   -   2012-05-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD  Organization address address: University Offices, Wellington Square city: OXFORD postcode: OX1 2JD contact info Titolo: Ms. Nome: Linda Cognome: Pialek Email: send email Telefono: +44 1865 289811 Fax: +44 1865 289801	UK (OXFORD)	coordinator	180˙783.75

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 Obiettivo del progetto (Objective)

'Of the estimated 400 ion channels encoded in the human genome, ~70 are potassium (K) channels. Their implication in a number of human diseases, e.g., cardiac arrhythmia, cystic fibrosis, makes K channels relevant drug targets. Whereas the number of high-resolution structures of membrane proteins has consistently increased over the last few years, their functional characterization using screening approaches has not kept pace with that of water-soluble proteins. In this context, reliable and informative rapid screening assays for membrane proteins are needed. By further developing the recently reported droplet interface bilayer (DIB) system, I intend to develop a rapid automatable platform for screening wild-type channels as well as libraries of mutant ion channels using Kcv (viral), KvAP (prokaryotic) and Kv1.2 (eukaryotic) as model systems. My blueprint for such a screening device is analogous to an assembly line consisting of a network of microchannels to; (i) construct lipid monolayer-encased aqueous droplets, (ii) synthesize ion channels inside these nanobioreactors by coupled in vitro transcription-translation (IVTT), (iii) form bilayers with other droplets containing channel blockers, (iv) subsequently measure single-channel conductance to determine activity. The assay will be capable of screening one channel against hundreds of blockers or screen a library of mutants against one or a few blockers. The development of this nanoscale-streamlined process offers the possibility of producing powerful lab-on-chip instruments for membrane protein assays, which have previously proven intractable.'