MIVFC

Development of a multichannel in vivo flow cytometer for the dynamic monitoring of circulating cells

Coordinatore	CYPRUS UNIVERSITY OF TECHNOLOGY    more  Organization address address: ARCHBISHOP KYPRIANOS LIMASSOL SAVINGS CO OPERATIVE BANK BUILDING 3RD FLOOR 31 city: LIMASSOL postcode: 3036 contact info Titolo: Dr. Nome: Andreas Cognome: Anayiotos Email: send email Telefono: +357 25002563 Fax: +357 25002657
Nazionalità Coordinatore	Cyprus [CY]
Totale costo	50˙000 €
EC contributo	50˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-RG
Funding Scheme	MC-IRG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-04-01   -   2012-03-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	CYPRUS UNIVERSITY OF TECHNOLOGY  Organization address address: ARCHBISHOP KYPRIANOS LIMASSOL SAVINGS CO OPERATIVE BANK BUILDING 3RD FLOOR 31 city: LIMASSOL postcode: 3036 contact info Titolo: Dr. Nome: Andreas Cognome: Anayiotos Email: send email Telefono: +357 25002563 Fax: +357 25002657	CY (LIMASSOL)	coordinator	50˙000.00

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 Obiettivo del progetto (Objective)

'We propose the development of a novel multichannel in vivo flow cytometer for the dynamic monitoring of multiple cell populations, such as tumor-associated cells or leukocytes, in the circulation of mice, for extended periods of time and without the need to extract blood samples. The design of the system is based on the confocal excitation and detection of fluorescently labelled cells as they flow through a blood vessel that is simultaneously probed by three lasers at different wavelengths. The feasibility and instrument sensitivity for detecting and enumerating cells in circulation will be investigated using both fluorescent microspheres and fluorescently labeled red blood cells that will be isolated from mouse whole blood samples. The ability of the system to track circulating cells in real time and in a non-invasive manner, will thus open up enormous possibilities for new investigations into the mechanisms that govern the complex trafficking and tissue interactions of these cells in a variety of clinical and biological fields such as cancer, stem cell biology and immunology. The system will also be able to measure flow velocities in vivo, in order to account for changes in circulating cell numbers that arise from changes in blood flow rate. We further propose to utilize the novel system in the dynamic monitoring of circulating cancer cells in an animal model of multiple myeloma. Fluorescently labeled multiple myeloma cells, obtained from cell cultures, will be injected in the circulation of mice to determine the instrument detection sensitivity as well as to assess the system capabilities in monitoring changes in the circulating cancer cell numbers due to tumor growth or following therapeutic intervention. The ability to quantitatively determine changes in these circulating cancer cell numbers in an in vivo manner can potentially be utilized to assess tumor burden in animals in order to track disease progression and monitor response to therapy.'