HAR1MC
Structure determination of human and chimpanzee HAR1F RNA by NMR
| Coordinatore | JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT AM MAIN
Organization address
address: GRUNEBURGPLATZ 1 contact info |
| Nazionalità Coordinatore | Germany [DE] |
| Totale costo | 168˙969 € |
| EC contributo | 168˙969 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2009-IEF |
| Funding Scheme | MC-IEF |
| Anno di inizio | 2010 |
| Periodo (anno-mese-giorno) | 2010-07-01 - 2012-06-30 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT AM MAIN
Organization address
address: GRUNEBURGPLATZ 1 contact info |
DE (FRANKFURT AM MAIN) | coordinator | 168˙969.00 |
Mappa
Word cloud
Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.
Obiettivo del progetto (Objective)
'The 118-nt long human accelerated region 1 of humans (hHAR1F) possesses the largest genetic drift of all 49 so far identified HARs with an estimated 18 substitutions in comparison to the homologous sequence from the chimpanzee (cHAR1F). In order to understand the unknown function of these non-coding RNAs in the development of human consciousness it is essential to elucidate their secondary and tertiary structures of the HAR RNA with atomic resolution. We will design RNA constructs which will mimic sub-domains of the whole cHAR1F and hHAR1F regions. Afterwards we will biochemically prepare these RNA constructs. On the basis of NMR spectroscopic investigations of these RNAs (resonance assignment, measurement of RDC data, 13C-relaxation and relaxation dispersion) we will determine models for the tertiary structures and study their dynamics. Individual mutations of the cHAR1F sequence toward the human sequence will give information, which of the 18 substitutions triggers the change in conformation found in hHAR1F RNA. The knowledge of the functions of the cHAR1F and hHAR1F RNA could give an explanation about the cerebral cortex development of humans in comparison to that of the chimpanzee and make a contribution to understanding which determinants are characteristic for the humans.'