HAR1MC

Structure determination of human and chimpanzee HAR1F RNA by NMR

Coordinatore	JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT AM MAIN    more  Organization address address: GRUNEBURGPLATZ 1 city: FRANKFURT AM MAIN postcode: 60323 contact info Titolo: Ms. Nome: Mareike Cognome: Schmitt Email: send email Telefono: 496980000000 Fax: 496980000000
Nazionalità Coordinatore	Germany [DE]
Totale costo	168˙969 €
EC contributo	168˙969 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-IEF
Funding Scheme	MC-IEF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-07-01   -   2012-06-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT AM MAIN  Organization address address: GRUNEBURGPLATZ 1 city: FRANKFURT AM MAIN postcode: 60323 contact info Titolo: Ms. Nome: Mareike Cognome: Schmitt Email: send email Telefono: 496980000000 Fax: 496980000000	DE (FRANKFURT AM MAIN)	coordinator	168˙969.00

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 Obiettivo del progetto (Objective)

'The 118-nt long human accelerated region 1 of humans (hHAR1F) possesses the largest genetic drift of all 49 so far identified HARs with an estimated 18 substitutions in comparison to the homologous sequence from the chimpanzee (cHAR1F). In order to understand the unknown function of these non-coding RNAs in the development of human consciousness it is essential to elucidate their secondary and tertiary structures of the HAR RNA with atomic resolution. We will design RNA constructs which will mimic sub-domains of the whole cHAR1F and hHAR1F regions. Afterwards we will biochemically prepare these RNA constructs. On the basis of NMR spectroscopic investigations of these RNAs (resonance assignment, measurement of RDC data, 13C-relaxation and relaxation dispersion) we will determine models for the tertiary structures and study their dynamics. Individual mutations of the cHAR1F sequence toward the human sequence will give information, which of the 18 substitutions triggers the change in conformation found in hHAR1F RNA. The knowledge of the functions of the cHAR1F and hHAR1F RNA could give an explanation about the cerebral cortex development of humans in comparison to that of the chimpanzee and make a contribution to understanding which determinants are characteristic for the humans.'