PROTEINARCHEOLOGY

protein archeology : reconstructed ancestors for protein engineering and crystallography

 Coordinatore WEIZMANN INSTITUTE OF SCIENCE 

 Organization address address: HERZL STREET 234
city: REHOVOT
postcode: 7610001

contact info
Titolo: Ms.
Nome: Talia
Cognome: Tzahor
Email: send email
Telefono: -3996
Fax: 972-8-934-4118

 Nazionalità Coordinatore Israel [IL]
 Totale costo 174˙653 €
 EC contributo 174˙653 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2009-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-10-01   -   2012-09-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    WEIZMANN INSTITUTE OF SCIENCE

 Organization address address: HERZL STREET 234
city: REHOVOT
postcode: 7610001

contact info
Titolo: Ms.
Nome: Talia
Cognome: Tzahor
Email: send email
Telefono: -3996
Fax: 972-8-934-4118

IL (REHOVOT) coordinator 174˙653.00

Mappa

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 Word cloud

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families    resurrections    vertebrate    structural    quenching    bacterial    evolution    ancestors    determination    ancestor    quorum    pon    pons    protein    divergence   

 Obiettivo del progetto (Objective)

'Advances in molecular evolution led to the novel concept of ancestral resurrections, or reconstructions. These allow the exploration of the mechanisms by which new protein families diverged. Reconstructed ancestors are also inherently more prone to functional divergence, and exhibit higher thermal stability, making them more tolerant to mutations, and potentially more evolvable. However, to date, very few ancestors have been resurrected, and the experimental methodologies are still lacking. I aim to develop and apply ancestor resurrections techniques. My first model will be the serum paraoxonase (PON) family. Widespread in vertebrates and bacteria, PONs’ physiological roles span from preventing atherosclerosis to bacterial quorum quenching. Going backwards into PON’s history, we will reconstruct PON’s last common vertebrate ancestor, and acquire insights regarding the divergence of various PON families. The ancestor’s properties (enzymatic and structural) may inform us, for example, how vertebrate PONs emerged from bacterial PONs that acted in quorum quenching. The ancestor, stable and soluble, would also provide an ideal starting point for directed evolution and structural determination. Once the methodologies are developed, other proteins will be subjected to a similar study with the aim of turning ancestor resurrections into a general and powerful tool for protein engineering and structural determination.'

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