NMEN-PBP

Functional organisation of penicillin binding proteins of Neisseria meningitidis

Coordinatore	NARODOWY INSTYTUT LEKOW    more  Organization address address: ULICA CHELMSKA 30/34 city: WARSZAWA postcode: 00 725 contact info Titolo: Ms. Nome: Renata Cognome: Cellmer Email: send email Telefono: 48228514376 Fax: 48228514376
Nazionalità Coordinatore	Poland [PL]
Totale costo	45˙000 €
EC contributo	45˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-ERG-2008
Funding Scheme	MC-ERG
Anno di inizio	2009
Periodo (anno-mese-giorno)	2009-11-01   -   2012-10-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	NARODOWY INSTYTUT LEKOW  Organization address address: ULICA CHELMSKA 30/34 city: WARSZAWA postcode: 00 725 contact info Titolo: Ms. Nome: Renata Cognome: Cellmer Email: send email Telefono: 48228514376 Fax: 48228514376	PL (WARSZAWA)	coordinator	45˙000.00

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 Obiettivo del progetto (Objective)

'Neisseria meningitidis (Nm) is a leading cause of bacterial meningitis and septicemia worldwide with a high case-fatality rate. Although penicillin remains the drug of choice in the treatment of IMD, an increasing number of meningococci with reduced susceptibility to penicillin have been emerging worldwide. Reduces susceptibility or resistance to penicillin in meningococci is connected to alterations in penicillin binding protein 2, PBP2. PBPs are involved in the late stages of peptidoglycan (PG) biosynthesis and therefore PBPs alterations might result in PG modifications and hence affect PG-dependent signalling. Until recently it was thought that N. meningitidis strains own only three PBPs, PBP1, PBP2 and PBP3. However, genomic analysis of the complete sequence of meningococcal strains revealed the presence of two other putative genes encoding PBP4 and PBP5 which can possess D,D-carboxypeptidase and endopeptidase activity. And indeed the results obtained during the previous Marie Curie IEF from the PG structure study as well as from animal model provide the first indication that putative PBP4 and PBP5 are functional in N. meningitidis, play a role in the PG synthesis and influence the virulence of meningococcal isolates. It has been proposed for some bacteria that different enzymes involved in PG biosynthesis might act in concert or may form more active dimmer forms in vivo. Therefore the aim of this project is to study the interactions between penicillin binding proteins of Neisseria meningitidis and their functional organisation. The project will use a multidisciplinary approach applying bacteriological, molecular and biochemical methods as well as electron microscopy. The research should give a possibility to determine interactions between different PBPs of meningococci and to elucidate a role of different parts/domains of PBPs through the assessment of their influence on structural modification of N. meningitidis PG.'