DROSOPHILA PIRNAS

Molecular mechanisms and biological role of the somatic piRNA/Piwi pathway – an RNA based genome immune system

 Coordinatore INSTITUT FUER MOLEKULARE BIOTECHNOLOGIE GMBH 

 Organization address address: Dr Bohrgasse 3
city: VIENNA
postcode: 1030

contact info
Titolo: Ms.
Nome: Tanja
Cognome: Winkler
Email: send email
Telefono: +43 1790444410
Fax: +43 179044110

 Nazionalità Coordinatore Austria [AT]
 Totale costo 225˙873 €
 EC contributo 225˙873 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2009-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-04-01   -   2012-03-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    INSTITUT FUER MOLEKULARE BIOTECHNOLOGIE GMBH

 Organization address address: Dr Bohrgasse 3
city: VIENNA
postcode: 1030

contact info
Titolo: Ms.
Nome: Tanja
Cognome: Winkler
Email: send email
Telefono: +43 1790444410
Fax: +43 179044110

AT (VIENNA) coordinator 225˙873.60

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

piwi    pathway    genome    host    pirna    germline    mechanistic    cells    silences    threat    uncontrolled    retroviruses    first    pirnas    endogenous    rnai    transposons    retroviral    functionally    somatic    caused    genomic   

 Obiettivo del progetto (Objective)

'All eukaryotic genomes contain mobile genetic elements called transposons. They can be described as “endo-parasites” at the genomic level and come in a variety of classes including endogenous retroviruses. The uncontrolled spread of transposons to new genomic sites poses a serious threat, e.g. due to insertional mutagenesis. How the host functionally silences transposons remained mysterious for a long time. Recently, the piRNA pathway, a germline specific RNAi pathway has been shown to be at the heart of the animal transposon control system. In Drosophila two separate piRNA pathways exist: One is active in germline cells and the other in somatic cells of the gonad. Interestingly, the somatic piRNA pathway protects the neighboring germline from endogenous retroviruses that invade the developing gamete via viral particles. Only two loci have a described role in this pathway: the Argonaute protein Piwi and the heterochromatic flamenco locus, source of the involved small RNAs (piRNAs). We entirely lack a mechanistic understanding of the biogenesis of somatic piRNAs and how the Piwi complex silences transposons. Here, we first propose to identify new components of the somatic piRNA pathway by two RNAi screens, one in cell culture and one in flies. Our long-term goal is to dissect the mechanistic makeup of the somatic piRNA pathway, its molecular constituents and their biochemical function. Secondly, we will set out to investigate the consequences for gene expression and genome integrity caused by ablating the somatic piRNA pathway. We will use high throughput sequencing technologies to address the following questions: Which retroviral elements become functionally expressed in the absence of Piwi? What is the damage for the host genome caused by uncontrolled retroviral activity? This will be the first systematic, qualitative and quantitative description of the threat posed by endogenous retroviruses and the importance of the somatic piRNA pathway in silencing them.'

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