MIRNA

Metal Ions and Metal Ion Complexes Guiding Folding and Function of Single RNA Molecules

 Coordinatore  

Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.

 Nazionalità Coordinatore Non specificata
 Totale costo 1˙495˙729 €
 EC contributo 1˙495˙729 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2010-StG
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-12-01   -   2015-11-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    UNIVERSITAET ZUERICH

 Organization address address: Raemistrasse 71
city: ZURICH
postcode: 8006

contact info
Titolo: Prof.
Nome: Roland Karl Oliver
Cognome: Sigel
Email: send email
Telefono: 41446354652
Fax: 41446356802

CH (ZURICH) hostInstitution 1˙495˙729.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

complexes    rnas    single    guide    molecule    ions    nucleic    global    ribozymes    inorganic    ion    folding    riboswitches    smfret    influence    chemical    structure    chemistry    metal    rna    function   

 Obiettivo del progetto (Objective)

'RNAs play crucial roles in cellular metabolic processes, e.g. ribozymes in RNA-processing or riboswitches in the regulation of protein expression. Metal ions thereby guide and determine folding and function of every complex nucleic acid structure. Recently, it has become increasingly evident that RNA folding and catalysis are extremely sensitive to changes in concentration and nature of the metal ion involved as well as to single-atom changes in metal ion complexes. The elucidation of the specific binding of certain metal ions and their complexes by nucleic acids poses an enormous challenge. This recognition process must depend solely on basic coordination chemical principles but is poorly understood. The goal of this project is to understand the effect of metal ions and their complexes on local and global structure formation of single large RNAs: Specifically, the influence of metal ions on the assembly of the catalytic core of group II intron ribozymes as well as the influence of single corrin side chains of coenzyme B12 to induce the structural change of its 202 nucleotide long riboswitch will be characterized. Combining classical Inorganic, Coordination, Analytical, and Organic Chemistry with Biophysics, we will apply single molecule Förster Resonance Energy Transfer spectroscopy (smFRET) together with hydrolytic cleavage experiments and chemical synthesis. SmFRET studies allow us to investigate every molecule individually instead of a bulk signal and thus to observe also minor populations. Our results will reveal how single metal ions and ligand atoms guide and influence global structure, folding, and function of ribozymes and riboswitches, and promise to have a significant impact on Biological Inorganic Chemistry, RNA Biochemistry, as well as Medicinal Chemistry.'

Altri progetti dello stesso programma (FP7-IDEAS-ERC)

NETAMIL (2014)

Going from Hand to Hand – Networks of Intellectual Exchange in the Tamil Learned Traditions

Read More  

I-CAD (2014)

"Innovative Catalyst Design for Large-Scale, Sustainable Processes"

Read More  

NANO-ISLANDS (2013)

NANOSCALE ANALYSIS OF PROTEIN ISLANDS ON LYMPHOCYTES

Read More