Opendata, web and dolomites

HJMIGRA

Single-molecule analysis of Holliday-junction (HJ) migration by the human double-HJ dissolvasome

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 HJMIGRA project word cloud

Explore the words cloud of the HJMIGRA project. It provides you a very rough idea of what is the project "HJMIGRA" about.

hr    leads    rmi2    bacteriophage    mobile    helicases    repair    structure    supports    homologous    hjs    microscopy    questions    blm    underlying    roles    migrate    specialized    helicase    lambda    junction    dsbs    hemicatenate    chromosomal    recombination    family    single    btr    hj    branch    rearrangements    error    understand    genome    investigates    group    bloom    dna    inaccessible    provides    engagement    labeling    breaks    previously    exact    outlined    convergent    enzymatic    biophysical    components    dissolves    cleavage    mechanisms    holliday    dissolvasome    template    total    complex    dhj    independent    biochemical    subunits    human    elucidation    processive    decatenation    recq    final    resolved    solution    humans    regulatory    double    action    dissolved    follow    ends    syndrome    processed    critical    fluorescence    proteins    molecule    substrate    consists    topoisomerases    maintenance    concerted    generate    reflection    fluorescently    solely    techniques    tirf    top3a    molecular    position    free    microfluidics    broken    dissolution    rmi1    migration    combined    mechanism    stranded    internal    crossover   

Project "HJMIGRA" data sheet

The following table provides information about the project.

Coordinator
EOTVOS LORAND TUDOMANYEGYETEM 

Organization address
address: EGYETEM TER 1-3
city: BUDAPEST
postcode: 1053
website: www.elte.hu

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Hungary [HU]
 Project website http://mk-lab.org
 Total cost 146˙239 €
 EC max contribution 146˙239 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2017-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EOTVOS LORAND TUDOMANYEGYETEM HU (BUDAPEST) coordinator 146˙239.00

Map

 Project objective

The project outlined here investigates the molecular mechanisms of the critical final steps of homologous recombination (HR) based DNA repair, a pathway that supports the error-free repair of double-stranded DNA breaks (DSBs). In HR, the broken DNA ends are processed and homologous DNA provides a template for repair. Engagement of both processed ends leads to the formation of a double Holliday-junction (DHJ) structure. DHJ can be resolved by enzymatic cleavage or dissolved by the concerted action of a specialized group of helicases (RecQ-family helicases including Bloom’s syndrome helicase (BLM)) and Type I topoisomerases (e.g. TOP3A). In humans the ‘dissolvasome complex’ consists of BLM, TOP3A and regulatory proteins (RMI1, RMI2), called the BTR complex. The BTR complex dissolves DHJ by 1. convergent branch migration of the two independent HJs and 2. decatenation of the final hemicatenate structure. Thus, dissolution solely results non-crossover products, which is necessary to avoid chromosomal rearrangements. What is the mechanism of HJ migration? What are the exact roles of the subunits of the BTR complex? How long can a HJ migrate (i.e. how processive is the ‘dissolvasome’)? How specific is the DHJ migration to the BTR complex compared to other human RecQ helicases? Here we aim to address these questions by using state-of-the-art single-molecule and solution biophysical and biochemical techniques. We will generate a previously inaccessible mobile HJ substrate integrated into λ-bacteriophage DNA. We will follow the processes underlying HJ migration by fluorescently labeling the BTR complex, HJ position and DNA end in total internal reflection fluorescence (TIRF) microscopy combined with microfluidics. Elucidation of the detailed roles of the BTR components in HJ branch migration will help us to understand their roles in genome maintenance.

 Publications

year authors and title journal last update
List of publications.
2016 Máté Gyimesi, Gábor M. Harami, Zsuzsa S. Kocsis, Mihály Kovács
Recent adaptations of fluorescence techniques for the determination of mechanistic parameters of helicases and translocases
published pages: 24-39, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2016.04.028
Methods 108 2019-07-23
2017 Gábor M. Harami, Yeonee Seol, Junghoon In, Veronika Ferencziová, Máté Martina, Máté Gyimesi, Kata Sarlós, Zoltán J. Kovács, Nikolett T. Nagy, Yuze Sun, Tibor Vellai, Keir C. Neuman, Mihály Kovács
Shuttling along DNA and directed processing of D-loops by RecQ helicase support quality control of homologous recombination
published pages: E466-E475, ISSN: 0027-8424, DOI: 10.1073/pnas.1615439114
Proceedings of the National Academy of Sciences 114/4 2019-07-23
2017 Mate Gyimesi, Zoltan Kovacs, Mihaly Kovacs
Holliday Junction Structure Development for Single-Molecule Visualization
published pages: 371a-372a, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2016.11.2017
Biophysical Journal 112/3 2019-07-23

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "HJMIGRA" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "HJMIGRA" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

TARGET SLEEP (2020)

Boosting motor learning through sleep and targeted memory reactivation in ageing and Parkinson’s disease

Read More  

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

CP-FTmmW Aminogen (2020)

Chemistry and structure of aminogen radicals using chirped-pulse Fourier transform (sub)millimeter rotational spectroscopy

Read More