HJMIGRA
Single-molecule analysis of Holliday-junction (HJ) migration by the human double-HJ dissolvasome
Total Cost €
0EC-Contrib. €
0Partnership
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Project "HJMIGRA" data sheet
The following table provides information about the project.
| Coordinator |
EOTVOS LORAND TUDOMANYEGYETEM
Organization address contact info |
| Coordinator Country | Hungary [HU] |
| Project website | http://mk-lab.org |
| Total cost | 146˙239 € |
| EC max contribution | 146˙239 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2014 |
| Funding Scheme | MSCA-IF-EF-RI |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-05-01 to 2017-04-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | EOTVOS LORAND TUDOMANYEGYETEM | HU (BUDAPEST) | coordinator | 146˙239.00 |
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Project objective
The project outlined here investigates the molecular mechanisms of the critical final steps of homologous recombination (HR) based DNA repair, a pathway that supports the error-free repair of double-stranded DNA breaks (DSBs). In HR, the broken DNA ends are processed and homologous DNA provides a template for repair. Engagement of both processed ends leads to the formation of a double Holliday-junction (DHJ) structure. DHJ can be resolved by enzymatic cleavage or dissolved by the concerted action of a specialized group of helicases (RecQ-family helicases including Bloom’s syndrome helicase (BLM)) and Type I topoisomerases (e.g. TOP3A). In humans the ‘dissolvasome complex’ consists of BLM, TOP3A and regulatory proteins (RMI1, RMI2), called the BTR complex. The BTR complex dissolves DHJ by 1. convergent branch migration of the two independent HJs and 2. decatenation of the final hemicatenate structure. Thus, dissolution solely results non-crossover products, which is necessary to avoid chromosomal rearrangements. What is the mechanism of HJ migration? What are the exact roles of the subunits of the BTR complex? How long can a HJ migrate (i.e. how processive is the ‘dissolvasome’)? How specific is the DHJ migration to the BTR complex compared to other human RecQ helicases? Here we aim to address these questions by using state-of-the-art single-molecule and solution biophysical and biochemical techniques. We will generate a previously inaccessible mobile HJ substrate integrated into λ-bacteriophage DNA. We will follow the processes underlying HJ migration by fluorescently labeling the BTR complex, HJ position and DNA end in total internal reflection fluorescence (TIRF) microscopy combined with microfluidics. Elucidation of the detailed roles of the BTR components in HJ branch migration will help us to understand their roles in genome maintenance.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2016 |
Máté Gyimesi, Gábor M. Harami, Zsuzsa S. Kocsis, Mihály Kovács Recent adaptations of fluorescence techniques for the determination of mechanistic parameters of helicases and translocases published pages: 24-39, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2016.04.028 |
Methods 108 | 2019-07-23 |
| 2017 |
Gábor M. Harami, Yeonee Seol, Junghoon In, Veronika Ferencziová, Máté Martina, Máté Gyimesi, Kata Sarlós, Zoltán J. Kovács, Nikolett T. Nagy, Yuze Sun, Tibor Vellai, Keir C. Neuman, Mihály Kovács Shuttling along DNA and directed processing of D-loops by RecQ helicase support quality control of homologous recombination published pages: E466-E475, ISSN: 0027-8424, DOI: 10.1073/pnas.1615439114 |
Proceedings of the National Academy of Sciences 114/4 | 2019-07-23 |
| 2017 |
Mate Gyimesi, Zoltan Kovacs, Mihaly Kovacs Holliday Junction Structure Development for Single-Molecule Visualization published pages: 371a-372a, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2016.11.2017 |
Biophysical Journal 112/3 | 2019-07-23 |
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