Opendata, web and dolomites

NMJALS SIGNED

In vivo analysis of neuromuscular junction stability in zebrafish models of amyotrophic lateral sclerosis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NMJALS project word cloud

Explore the words cloud of the NMJALS project. It provides you a very rough idea of what is the project "NMJALS" about.

stability    genetic    contexts    neuron    starts    disease    detecting    unrelated    amyotrophic    combine    precise    innovative    regulatory    constituting    maintenance    dynamic    multidisciplinary    unravel    death    fish    plan    appear    live    onset    patient    genes    degeneration    therapeutic    dynamics    clock    resolution    interesting    causes    progression    characterise    junctions    zebrafish    vertebrate    point    computational    quantification    lateral    patients    cytoskeleton    identification    super    proteins    neuronal    protein    slow    excellent    modifications    largely    relevance    defects    als    cell    destabilisation    equilibrium    preceding    tools    neuromuscular    turnover    understand    neurodegenerative    molecules    seem    stabilisation    therapies    model    regardless    sclerosis    organisation    fluorescent    adhesion    nmj    neurons    characterisation    technically    imaging    lethal    event    mechanisms    microscopy    analysing    subtle    individuals    quantitative    biology    motor    mutations    counteract    molecular    specificity   

Project "NMJALS" data sheet

The following table provides information about the project.

Coordinator
INSTITUT CURIE 

Organization address
address: rue d'Ulm 26
city: PARIS
postcode: 75231
website: www.curie.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-11-16   to  2017-11-15

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT CURIE FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Amyotrophic lateral sclerosis (ALS) is a late onset, lethal neurodegenerative disease of motor neurons that affects about 2 in 100,000 individuals per year. Different genetic mutations have been described in patients, but the genes involved seem unrelated and the causes of the disease appear complex and are still largely not understood. Of relevance, the destabilisation of neuromuscular junctions (NMJ) may be an early event, preceding neuronal death, making them an interesting therapeutic target to slow down disease progression. The goal of the study is to characterise the molecular organisation and dynamics of NMJ, in particular of cell adhesion molecules, in order to understand the mechanisms of maintenance of NMJ by analysing defects in their organisation in ALS contexts. Technically, we plan to combine neuron biology, quantitative cell biology and computational tools to reach a precise molecular understanding of the dynamic equilibrium of NMJ in zebrafish, an excellent vertebrate model for live imaging. We will develop innovative methods of super-resolution microscopy and of quantification of protein turnover using a fluorescent molecular clock. This precise molecular characterisation of the NMJ will allow detecting subtle early changes in the NMJ that occur before neuronal degeneration starts, in fish carrying mutations described in ALS patients. The project will lead to the identification of key proteins for NMJ stabilisation and enable the analysis of regulatory elements of this stability, especially the cytoskeleton. The innovative multidisciplinary approach of this study will unravel early modifications in the organisation of the NMJ preceding neuronal degeneration in ALS contexts. It will point out key proteins of NMJ maintenance constituting new targets for the development of therapies to counteract destabilisation of neuromuscular junctions, regardless of the genetic specificity of the patient.

 Publications

year authors and title journal last update
List of publications.
2017 Shahad Albadri, Filippo Del Bene, Céline Revenu
Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish
published pages: 77-85, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2017.03.005
Methods 121-122 2019-06-18

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NMJALS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NMJALS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MAGIMOX (2019)

Nanometre scale imaging of magnetic perovskite oxide thin films using scanning transmission electron microscopy

Read More  

Photonic Radar (2019)

Implementation of Long Reach Hybrid Photonic Radar System and convergence over FSO and PON Networks

Read More  

AsymmFlow (2020)

Go with the continuous flow: Asymmetric Synthesis of Bioactive Alkaloids by Multistep Continuous-Flow Processes

Read More