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Chap4Resp SIGNED

Catching in action a novel bacterial chaperone for respiratory complexes

Total Cost €


EC-Contrib. €






 Chap4Resp project word cloud

Explore the words cloud of the Chap4Resp project. It provides you a very rough idea of what is the project "Chap4Resp" about.

realize    techniques    cryogenic    transfer    bacterial    conjunction    mechanisms    insights    proton    huge    solved    complexes    enigmatic    metal    composition    14    light    data    gradient    electron    requiring    cluster    subunits    clusters    hydrolysis    interaction    homologue    coupling    mitochondria    conserved    causing    operates    biophysical    substrate    obtain    antibiotics    situ    ageing    flavin    life    proteins    function    maturation    structural    interactions    uncover    wire    functional    batteries    snapshots    flow    spectroscopic    provides    context    imaging    cryoem    waste    resistant    diseases    location    mechanism    membrane    subtomogram    power    reactive    respiration    cryo    model    insertion    stresses    coli    correlative    cellular    45    human    investigation    macromolecular    bacteria    scrutinized    multisubunit    gain    fuelled    factories    protein    combines    species    synergetic    biogenesis    multidisciplinary    cofactors    genetic    substrates    assembly    cage    microscopy    energy    plasticity    pumping    atp    structure    oxygen    inner    quasiatomic    cofactor    subunit    chaperone    cytoplasm    latest    respiratory    optical    minimal    fe   

Project "Chap4Resp" data sheet

The following table provides information about the project.


Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website
 Total cost 1˙999˙956 €
 EC max contribution 1˙999˙956 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-10-01   to  2021-09-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Cellular respiration provides energy to power essential processes of life. Respiratory complexes are macromolecular batteries coupling electron flow through a wire of metal clusters and cofactors with proton transfer across the inner membrane of mitochondria and bacteria. Waste products of these cellular factories are reactive oxygen species causing ageing and diseases. Assembly and maturation mechanisms of respiratory complexes remain enigmatic because of their membrane location, multisubunit composition and cofactor insertion. E. coli Complex I, one of the largest membrane proteins, composed of 14 conserved subunits with 9 Fe/S clusters and a flavin, is a minimal model for its 45-subunit human homologue. When proton pumping by respiratory complexes is affected, bacteria become resistant to antibiotics requiring proton gradient for uptake. Based on the latest genetic data, we realize that the huge E. coli macromolecular cage, the structure of which we recently solved by cryo-electron microscopy (cryoEM), in conjunction with a novel protein cofactor, is a specific chaperone for Fe/S cluster biogenesis and assembly of respiratory complexes. This integrated multidisciplinary project combines cryoEM and other structural, biophysical and spectroscopic techniques, to uncover the functional mechanism of this emerging chaperone. The structural plasticity of the chaperone fuelled by ATP hydrolysis, and its interaction with Fe/S cluster biogenesis systems and the main respiratory complexes as a function of stresses, will be scrutinized to gain quasiatomic insights into the way the chaperone operates on its substrates. A novel technology for synergetic in situ investigation of protein complexes in the bacterial cytoplasm by optical imaging, state-of-the-art cryogenic correlative light and electron microscopy, and subtomogram analysis, will be developed and used to obtain snapshots of the chaperone-substrate interactions in the cellular context.


year authors and title journal last update
List of publications.
2020 Alister Burt, C. Keith Cassidy, Peter Ames, Maria Bacia-Verloop, Megghane Baulard, Karine Huard, Zaida Luthey-Schulten, Ambroise Desfosses, Phillip J. Stansfeld, William Margolin, John S. Parkinson, Irina Gutsche
Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-020-14350-9
Nature Communications 11/1 2020-04-15
2019 Rosalba Lepore, Andriy Kryshtafovych, Markus Alahuhta, Harshul A. Veraszto, Yannick J. Bomble, Joshua C. Bufton, Alex N. Bullock, Cody Caba, Hongnan Cao, Owen R. Davies, Ambroise Desfosses, Matthew Dunne, Krzysztof Fidelis, Celia W. Goulding, Manickam Gurusaran, Irina Gutsche, Christopher J. Harding, Marcus D. Hartmann, Christopher S. Hayes, Andrzej Joachimiak, Petr G. Leiman, Peter Loppnau, Andre
Target highlights in CASP13: Experimental target structures through the eyes of their authors
published pages: 1037-1057, ISSN: 0887-3585, DOI: 10.1002/prot.25805
Proteins: Structure, Function, and Bioinformatics 87/12 2020-04-15
2019 Kandiah E, Carriel D, Garcia PS, Felix J, Banzhaf M, Kritikos G, Bacia-Verloop M, Brochier-Armanet C, Elsen S, Gutsche I.
Structure, Function, and Evolution of the Pseudomonas aeruginosa Lysine Decarboxylase LdcA
published pages: 1842-1854, ISSN: 0969-2126, DOI: 10.1016/j.str.2019.10.003
Structure Dec 3;27(12) 2020-04-15
2020 Matthew Jessop, Benoit Arragain, Roger Miras, Angélique Fraudeau, Karine Huard, Maria Bacia-Verloop, Patrice Catty, Jan Felix, Hélène Malet, Irina Gutsche
Structural insights into ATP hydrolysis by the MoxR ATPase RavA and the LdcI-RavA cage-like complex
published pages: , ISSN: 2399-3642, DOI: 10.1038/s42003-020-0772-0
Communications Biology 3/1 2020-04-15
2019 Ambroise Desfosses, Hariprasad Venugopal, Tapan Joshi, Jan Felix, Matthew Jessop, Hyengseop Jeong, Jaekyung Hyun, J. Bernard Heymann, Mark R. H. Hurst, Irina Gutsche, Alok K. Mitra
Atomic structures of an entire contractile injection system in both the extended and contracted states
published pages: 1885-1894, ISSN: 2058-5276, DOI: 10.1038/s41564-019-0530-6
Nature Microbiology 4/11 2020-04-15
2019 Firas Khatib, Ambroise Desfosses, Brian Koepnick, Jeff Flatten, Zoran Popović, David Baker, Seth Cooper, Irina Gutsche, Scott Horowitz
Building de novo cryo-electron microscopy structures collaboratively with citizen scientists
published pages: e3000472, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.3000472
PLOS Biology 17/11 2020-04-15
2019 Federica Laddomada, Mayara M. Miyachiro, Matthew Jessop, Delphine Patin, Viviana Job, Dominique Mengin-Lecreulx, Aline Le Roy, Christine Ebel, Cécile Breyton, Irina Gutsche, Andréa Dessen
The MurG glycosyltransferase provides an oligomeric scaffold for the cytoplasmic steps of peptidoglycan biosynthesis in the human pathogen Bordetella pertussis
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-019-40966-z
Scientific Reports 9/1 2020-04-15
2019 Koen H. G. Verschueren, Clement Blanchet, Jan Felix, Ann Dansercoer, Dirk De Vos, Yehudi Bloch, Jozef Van Beeumen, Dmitri Svergun, Irina Gutsche, Savvas N. Savvides, Kenneth Verstraete
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle
published pages: 571-575, ISSN: 0028-0836, DOI: 10.1038/s41586-019-1095-5
Nature 568/7753 2020-04-15
2016 Eaazhisai Kandiah, Diego Carriel, Julien Perard, Hélène Malet, Maria Bacia, Kaiyin Liu, Sze W. S. Chan, Walid A. Houry, Sandrine Ollagnier de Choudens, Sylvie Elsen, Irina Gutsche
Structural insights into the Escherichia coli lysine decarboxylases andmolecular determinants of interaction with the AAA+ ATPase RavA
published pages: , ISSN: 2045-2322, DOI: 10.1038/srep24601
Scientific Reports 6/1 2019-10-14
2018 Diego Carriel, Pierre Simon Garcia, Florence Castelli, Patricia Lamourette, François Fenaille, Céline Brochier-Armanet, Sylvie Elsen, Irina Gutsche
A novel subfamily of bacterial AAT-fold basic amino acid decarboxylases and functional characterization of its first representative: Pseudomonas aeruginosa LdcA
published pages: , ISSN: 1759-6653, DOI: 10.1093/gbe/evy228
Genome Biology and Evolution 2019-10-14

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