ABCvolume SIGNED
The ABC of Cell Volume Regulation
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Project "ABCvolume" data sheet
The following table provides information about the project.
| Coordinator |
RIJKSUNIVERSITEIT GRONINGEN
Organization address contact info |
| Coordinator Country | Netherlands [NL] |
| Project website | http://www.membraneenzymology.com |
| Total cost | 2˙247˙230 € |
| EC max contribution | 2˙247˙230 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2014-ADG |
| Funding Scheme | ERC-ADG |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-07-01 to 2020-06-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | RIJKSUNIVERSITEIT GRONINGEN | NL (GRONINGEN) | coordinator | 2˙247˙230.00 |
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Project objective
Cell volume regulation is crucial for any living cell because changes in volume determine the metabolic activity through e.g. changes in ionic strength, pH, macromolecular crowding and membrane tension. These physical chemical parameters influence interaction rates and affinities of biomolecules, folding rates, and fold stabilities in vivo. Understanding of the underlying volume regulatory mechanisms has immediate application in biotechnology and health, yet these factors are generally ignored in systems analyses of cellular functions.
My team has uncovered a number of mechanisms and insights of cell volume regulation. The next step forward is to elucidate how the components of a cell volume regulatory circuit work together and control the physicochemical conditions of the cell.
I propose construction of a synthetic cell in which an osmoregulatory transporter and mechanosensitive channel form a minimal volume regulatory network. My group has developed the technology to reconstitute membrane proteins into lipid vesicles (synthetic cells). One of the challenges is to incorporate into the vesicles an efficient pathway for ATP production and maintain energy homeostasis while the load on the system varies. We aim to control the transmembrane flux of osmolytes, which requires elucidation of the molecular mechanism of gating of the osmoregulatory transporter. We will focus on the glycine betaine ABC importer, which is one of the most complex transporters known to date with ten distinct protein domains, transiently interacting with each other.
The proposed synthetic metabolic circuit constitutes a fascinating out-of-equilibrium system, allowing us to understand cell volume regulatory mechanisms in a context and at a level of complexity minimally needed for life. Analysis of this circuit will address many outstanding questions and eventually allow us to design more sophisticated vesicular systems with applications, for example as compartmentalized reaction networks.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2019 |
Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevitt, Thorben Cordes Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.44652 |
eLife 8 | 2020-03-20 |
| 2019 |
Boqun Liu, Zarief Hasrat, Bert Poolman, Arnold J. Boersma Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress published pages: , ISSN: 0021-9193, DOI: 10.1128/jb.00708-18 |
Journal of Bacteriology 201/10 | 2020-03-20 |
| 2020 |
Åukasz Syga, Reinder H. Vries, Hugo Oosterhout, Rianne Bartelds, Arnold J. Boersma, Gerard Roelfes, Bert Poolman A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201900655 |
ChemBioChem | 2020-03-20 |
| 2020 |
Matteo Gabba, Bert Poolman Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift published pages: 435-447, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2019.11.3383 |
Biophysical Journal 118/2 | 2020-03-20 |
| 2019 |
Hendrik R. Sikkema, Bauke F. Gaastra, Tjeerd Pols, Bert Poolman Cell Fuelling and Metabolic Energy Conservation in Synthetic Cells published pages: 2581-2592, ISSN: 1439-4227, DOI: 10.1002/cbic.201900398 |
ChemBioChem 20/20 | 2020-03-20 |
| 2019 |
Wojciech Mikołaj Śmigiel, Pauline Lefrançois, Bert Poolman Physicochemical considerations for bottom-up synthetic biology published pages: 445-458, ISSN: 2397-8554, DOI: 10.1042/etls20190017 |
Emerging Topics in Life Sciences 3/5 | 2020-03-20 |
| 2020 |
Matteo Gabba, Jacopo Frallicciardi, Joury van ’t Klooster, Ryan Henderson, Åukasz Syga, Robert Mans, Antonius J.A. van Maris, Bert Poolman Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane published pages: 422-434, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2019.11.3384 |
Biophysical Journal 118/2 | 2020-03-20 |
| 2019 |
Tjeerd Pols, Hendrik R. Sikkema, Bauke F. Gaastra, Jacopo Frallicciardi, Wojciech M. Åšmigiel, Shubham Singh, Bert Poolman A synthetic metabolic network for physicochemical homeostasis published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12287-2 |
Nature Communications 10/1 | 2020-03-20 |
| 2017 |
Boqun Liu, Bert Poolman, Arnold J. Boersma Ionic Strength Sensing in Living Cells published pages: 2510-2514, ISSN: 1554-8929, DOI: 10.1021/acschembio.7b00348 |
ACS Chemical Biology 12/10 | 2020-03-20 |
| 2017 |
Jonas van den Berg, Arnold J. Boersma, Bert Poolman Microorganisms maintain crowding homeostasis published pages: 309-318, ISSN: 1740-1526, DOI: 10.1038/nrmicro.2017.17 |
Nature Reviews Microbiology 15/5 | 2020-03-20 |
| 2018 |
Rianne Bartelds, Mohammad Hadi Nematollahi, Tjeerd Pols, Marc C. A. Stuart, Abbas Pardakhty, Gholamreza Asadikaram, Bert Poolman Niosomes, an alternative for liposomal delivery published pages: e0194179, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0194179 |
PLOS ONE 13/4 | 2020-03-20 |
| 2016 |
Giel H. Scheepers, Jelger A. Lycklama a Nijeholt, Bert Poolman An updated structural classification of substrate-binding proteins published pages: 4393-4401, ISSN: 0014-5793, DOI: 10.1002/1873-3468.12445 |
FEBS Letters 590/23 | 2020-03-20 |
| 2018 |
Gea K. Schuurman-Wolters, Marijn de Boer, Martyna K. Pietrzyk, Bert Poolman Protein Linkers Provide Limits on the Domain Interactions in the ABC Importer GlnPQ and Determine the Rate of Transport published pages: 1249-1262, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2018.02.014 |
Journal of Molecular Biology 430/8 | 2020-03-20 |
| 2016 |
Stephanie Ruiz, Gea Schuurman-Wolters, Bert Poolman Crystal Structure of the Substrate-Binding Domain from Listeria monocytogenes Bile-Resistance Determinant BilE published pages: 162, ISSN: 2073-4352, DOI: 10.3390/cryst6120162 |
Crystals 6/12 | 2020-03-20 |
| 2017 |
Boqun Liu, Christoffer Ã…berg, Floris J. van Eerden, Siewert J. Marrink, Bert Poolman, Arnold J. Boersma Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding published pages: 1929-1939, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2017.04.004 |
Biophysical Journal 112/9 | 2020-03-20 |
| 2017 |
Paul E Schavemaker, Wojciech M Åšmigiel, Bert Poolman Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.30084 |
eLife 6 | 2020-03-20 |
| 2017 |
Veerle Van Meervelt, Misha Soskine, Shubham Singh, Gea K. Schuurman-Wolters, Hein J. Wijma, Bert Poolman, Giovanni Maglia Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor published pages: 18640-18646, ISSN: 0002-7863, DOI: 10.1021/jacs.7b10106 |
Journal of the American Chemical Society 139/51 | 2020-03-20 |
| 2018 |
Frans Bianchi, Åukasz Syga, Gemma Moiset, Dian Spakman, Paul E. Schavemaker, Christiaan M. Punter, Anne-Bart Seinen, Antoine M. van Oijen, Andrew Robinson, Bert Poolman Steric exclusion and protein conformation determine the localization of plasma membrane transporters published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-02864-2 |
Nature Communications 9/1 | 2020-03-20 |
| 2018 |
Jelger A. Lycklama a Nijeholt, Ruslan Vietrov, Gea K. Schuurman-Wolters, Bert Poolman Energy Coupling Efficiency in the Type I ABC Transporter GlnPQ published pages: 853-866, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2018.02.001 |
Journal of Molecular Biology 430/6 | 2020-03-20 |
| 2017 |
Linda E. Franken, Gert T. Oostergetel, Tjaard Pijning, Pranav Puri, Valentina Arkhipova, Egbert J. Boekema, Bert Poolman, Albert Guskov A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-00718-x |
Nature Communications 8/1 | 2020-03-20 |
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