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The ABC of Cell Volume Regulation

Total Cost €


EC-Contrib. €






 ABCvolume project word cloud

Explore the words cloud of the ABCvolume project. It provides you a very rough idea of what is the project "ABCvolume" about.

ignored    date    mechanism    abc    questions    affinities    macromolecular    crowding    minimal    osmoregulatory    flux    domains    uncovered    life    underlying    reconstitute    team    stabilities    interacting    channel    insights    fold    load    context    transporter    constitutes    form    atp    glycine    protein    compartmentalized    gating    cellular    osmolytes    cells    membrane    regulation    interaction    biotechnology    outstanding    transiently    rates    regulatory    functions    transporters    ten    health    vesicles    cell    understand    synthetic    proteins    mechanosensitive    ionic    varies    components    sophisticated    influence    lipid    physical    transmembrane    strength    generally    mechanisms    vesicular    vivo    equilibrium    networks    elucidate    betaine    network    reaction    biomolecules    fascinating    efficient    complexity    ph    molecular    construction    chemical    incorporate    energy    elucidation    homeostasis    minimally    folding    importer    metabolic    living    circuit    group    tension    physicochemical    volume   

Project "ABCvolume" data sheet

The following table provides information about the project.


Organization address
address: Broerstraat 5
postcode: 9712CP

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website
 Total cost 2˙247˙230 €
 EC max contribution 2˙247˙230 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-ADG
 Funding Scheme ERC-ADG
 Starting year 2015
 Duration (year-month-day) from 2015-07-01   to  2020-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Cell volume regulation is crucial for any living cell because changes in volume determine the metabolic activity through e.g. changes in ionic strength, pH, macromolecular crowding and membrane tension. These physical chemical parameters influence interaction rates and affinities of biomolecules, folding rates, and fold stabilities in vivo. Understanding of the underlying volume regulatory mechanisms has immediate application in biotechnology and health, yet these factors are generally ignored in systems analyses of cellular functions.

My team has uncovered a number of mechanisms and insights of cell volume regulation. The next step forward is to elucidate how the components of a cell volume regulatory circuit work together and control the physicochemical conditions of the cell.

I propose construction of a synthetic cell in which an osmoregulatory transporter and mechanosensitive channel form a minimal volume regulatory network. My group has developed the technology to reconstitute membrane proteins into lipid vesicles (synthetic cells). One of the challenges is to incorporate into the vesicles an efficient pathway for ATP production and maintain energy homeostasis while the load on the system varies. We aim to control the transmembrane flux of osmolytes, which requires elucidation of the molecular mechanism of gating of the osmoregulatory transporter. We will focus on the glycine betaine ABC importer, which is one of the most complex transporters known to date with ten distinct protein domains, transiently interacting with each other.

The proposed synthetic metabolic circuit constitutes a fascinating out-of-equilibrium system, allowing us to understand cell volume regulatory mechanisms in a context and at a level of complexity minimally needed for life. Analysis of this circuit will address many outstanding questions and eventually allow us to design more sophisticated vesicular systems with applications, for example as compartmentalized reaction networks.


year authors and title journal last update
List of publications.
2019 Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevitt, Thorben Cordes
Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers
published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.44652
eLife 8 2020-03-20
2019 Boqun Liu, Zarief Hasrat, Bert Poolman, Arnold J. Boersma
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
published pages: , ISSN: 0021-9193, DOI: 10.1128/jb.00708-18
Journal of Bacteriology 201/10 2020-03-20
2020 Łukasz Syga, Reinder H. Vries, Hugo Oosterhout, Rianne Bartelds, Arnold J. Boersma, Gerard Roelfes, Bert Poolman
A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes
published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201900655
ChemBioChem 2020-03-20
2020 Matteo Gabba, Bert Poolman
Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift
published pages: 435-447, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2019.11.3383
Biophysical Journal 118/2 2020-03-20
2019 Hendrik R. Sikkema, Bauke F. Gaastra, Tjeerd Pols, Bert Poolman
Cell Fuelling and Metabolic Energy Conservation in Synthetic Cells
published pages: 2581-2592, ISSN: 1439-4227, DOI: 10.1002/cbic.201900398
ChemBioChem 20/20 2020-03-20
2019 Wojciech Mikołaj Śmigiel, Pauline Lefrançois, Bert Poolman
Physicochemical considerations for bottom-up synthetic biology
published pages: 445-458, ISSN: 2397-8554, DOI: 10.1042/etls20190017
Emerging Topics in Life Sciences 3/5 2020-03-20
2020 Matteo Gabba, Jacopo Frallicciardi, Joury van ’t Klooster, Ryan Henderson, Łukasz Syga, Robert Mans, Antonius J.A. van Maris, Bert Poolman
Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane
published pages: 422-434, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2019.11.3384
Biophysical Journal 118/2 2020-03-20
2019 Tjeerd Pols, Hendrik R. Sikkema, Bauke F. Gaastra, Jacopo Frallicciardi, Wojciech M. Åšmigiel, Shubham Singh, Bert Poolman
A synthetic metabolic network for physicochemical homeostasis
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12287-2
Nature Communications 10/1 2020-03-20
2017 Boqun Liu, Bert Poolman, Arnold J. Boersma
Ionic Strength Sensing in Living Cells
published pages: 2510-2514, ISSN: 1554-8929, DOI: 10.1021/acschembio.7b00348
ACS Chemical Biology 12/10 2020-03-20
2017 Jonas van den Berg, Arnold J. Boersma, Bert Poolman
Microorganisms maintain crowding homeostasis
published pages: 309-318, ISSN: 1740-1526, DOI: 10.1038/nrmicro.2017.17
Nature Reviews Microbiology 15/5 2020-03-20
2018 Rianne Bartelds, Mohammad Hadi Nematollahi, Tjeerd Pols, Marc C. A. Stuart, Abbas Pardakhty, Gholamreza Asadikaram, Bert Poolman
Niosomes, an alternative for liposomal delivery
published pages: e0194179, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0194179
PLOS ONE 13/4 2020-03-20
2016 Giel H. Scheepers, Jelger A. Lycklama a Nijeholt, Bert Poolman
An updated structural classification of substrate-binding proteins
published pages: 4393-4401, ISSN: 0014-5793, DOI: 10.1002/1873-3468.12445
FEBS Letters 590/23 2020-03-20
2018 Gea K. Schuurman-Wolters, Marijn de Boer, Martyna K. Pietrzyk, Bert Poolman
Protein Linkers Provide Limits on the Domain Interactions in the ABC Importer GlnPQ and Determine the Rate of Transport
published pages: 1249-1262, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2018.02.014
Journal of Molecular Biology 430/8 2020-03-20
2016 Stephanie Ruiz, Gea Schuurman-Wolters, Bert Poolman
Crystal Structure of the Substrate-Binding Domain from Listeria monocytogenes Bile-Resistance Determinant BilE
published pages: 162, ISSN: 2073-4352, DOI: 10.3390/cryst6120162
Crystals 6/12 2020-03-20
2017 Boqun Liu, Christoffer Ã…berg, Floris J. van Eerden, Siewert J. Marrink, Bert Poolman, Arnold J. Boersma
Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding
published pages: 1929-1939, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2017.04.004
Biophysical Journal 112/9 2020-03-20
2017 Paul E Schavemaker, Wojciech M Åšmigiel, Bert Poolman
Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.30084
eLife 6 2020-03-20
2017 Veerle Van Meervelt, Misha Soskine, Shubham Singh, Gea K. Schuurman-Wolters, Hein J. Wijma, Bert Poolman, Giovanni Maglia
Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor
published pages: 18640-18646, ISSN: 0002-7863, DOI: 10.1021/jacs.7b10106
Journal of the American Chemical Society 139/51 2020-03-20
2018 Frans Bianchi, Łukasz Syga, Gemma Moiset, Dian Spakman, Paul E. Schavemaker, Christiaan M. Punter, Anne-Bart Seinen, Antoine M. van Oijen, Andrew Robinson, Bert Poolman
Steric exclusion and protein conformation determine the localization of plasma membrane transporters
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-02864-2
Nature Communications 9/1 2020-03-20
2018 Jelger A. Lycklama a Nijeholt, Ruslan Vietrov, Gea K. Schuurman-Wolters, Bert Poolman
Energy Coupling Efficiency in the Type I ABC Transporter GlnPQ
published pages: 853-866, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2018.02.001
Journal of Molecular Biology 430/6 2020-03-20
2017 Linda E. Franken, Gert T. Oostergetel, Tjaard Pijning, Pranav Puri, Valentina Arkhipova, Egbert J. Boekema, Bert Poolman, Albert Guskov
A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-00718-x
Nature Communications 8/1 2020-03-20

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