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Laser-induced vapour nanobubbles for intracellular delivery of nanomaterials and treatment of biofilm infections

Total Cost €


EC-Contrib. €






 NANOBUBBLE project word cloud

Explore the words cloud of the NANOBUBBLE project. It provides you a very rough idea of what is the project "NANOBUBBLE" about.

nm    thermal    mechanical    endocytosed    hot    inducing    modulation    root    light    cell    nanobubbles    vnb    cancer    bubble    neighbouring    ups    lasers    violently    absorb    membrane    efficient    gold    canals    vivo    optical    found    functional    tissue    endosomal    effect    nanomedicine    disruptive    expand    functionalized    vnbs    hundred    nanoparticles    contrast    eradication    biosensing    medicine    laser    delivered    nano    escape    cells    aunp    emerge    tumorigenesis    therapy    force    mammalian    formulations    infections    damage    labelling    structures    convert    biofilms    enhancers    first    cytosol    microscopy    adoptive    lapse    water    macromolecules    perforation    dental    wounds    photothermal    extremely    collapses    throughput    picosecond    pulses    until    effector    cytosolic    confidential    vapor    imaging    causing    labelled    biofilm    time    chronic    intense    treatment    microfluidics    intracellular    energy    anti    subcellular    stem    particles    absorbing    pluripotent    aunps    generation   

Project "NANOBUBBLE" data sheet

The following table provides information about the project.


Organization address
city: GENT
postcode: 9000

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Project website
 Total cost 2˙236˙250 €
 EC max contribution 2˙236˙250 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2020-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITEIT GENT BE (GENT) coordinator 1˙980˙000.00
2    UNIVERSITE DE LILLE FR (LILLE) participant 256˙250.00


 Project objective

Lasers have found widespread application in medicine, such as for photothermal therapy. Gold nanoparticles (AuNPs), are often used as enhancers of the photothermal effect since they can efficiently absorb laser light and convert it into thermal energy. When absorbing intense nano- or picosecond laser pulses, AuNPs can become extremely hot and water vapor nanobubbles (VNBs) can emerge around these particles in tissue. A VNB will expand up to several hundred nm until the thermal energy from the AuNP is consumed, after which the bubble violently collapses, causing mechanical damage to neighbouring structures. In this project the aim is to make use of the disruptive mechanical force of VNBs to enable highly controlled and efficient delivery of macromolecules and nanoparticles in cells and biofilms. First, optical set-ups and microfluidics devices will be developed for high-throughput treatment of cells and biofilms. Second, VNBs will be used to achieve efficient cytosolic delivery of functional macromolecules in mammalian cells by cell membrane perforation or by inducing endosomal escape of endocytosed nanomedicine formulations that are functionalized with AuNPs. These concepts will be applied to tumorigenesis research, generation of induced pluripotent stem cells and modulation of effector T-cells for adoptive T-cell anti-cancer therapy. Third, contrast nanoparticles for cell imaging will be delivered into the cytosol of mammalian cells through VNB induced cell membrane perforation. This will enable more reliable in vivo imaging of labelled cells, labelling of subcellular structures for time-lapse microscopy and intracellular biosensing. Finally, [... confidential...] laser-induced VNBs will be used [... confidential...] for improved eradication of biofilms. This concept will be applied to biofilm infections in dental root canals and chronic wounds.


year authors and title journal last update
List of publications.
2020 Jing Liu, Tim Hebbrecht, Toon Brans, Eef Parthoens, Saskia Lippens, Chengnan Li, Herlinde De Keersmaecker, Winnok H. De Vos, Stefaan C. De Smedt, Rabah Boukherroub, Jan Gettemans, Ranhua Xiong, Kevin Braeckmans
Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation
published pages: 485-495, ISSN: 1998-0124, DOI: 10.1007/s12274-020-2633-z
Nano Research 13/2 2020-04-15
2018 Gaëlle Houthaeve, Ranhua Xiong, Joke Robijns, Bert Luyckx, Yasmine Beulque, Toon Brans, Coen Campsteijn, Sangram K. Samal, Stephan Stremersch, Stefaan C. De Smedt, Kevin Braeckmans, Winnok H. De Vos
Targeted Perturbation of Nuclear Envelope Integrity with Vapor Nanobubble-Mediated Photoporation
published pages: 7791-7802, ISSN: 1936-0851, DOI: 10.1021/acsnano.8b01860
ACS Nano 12/8 2020-04-15
2016 Ranhua Xiong, Roosmarijn E. Vandenbroucke, Katleen Broos, Toon Brans, Elien Van Wonterghem, Claude Libert, Jo Demeester, Stefaan C. De Smedt, Kevin Braeckmans
Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability
published pages: 12982, ISSN: 2041-1723, DOI: 10.1038/ncomms12982
Nature Communications 7 2019-06-06
2016 Ranhua Xiong, Freya Joris, Sayuan Liang, Riet De Rycke, Saskia Lippens, Jo Demeester, Andre Skirtach, Koen Raemdonck, Uwe Himmelreich, Stefaan C. De Smedt, Kevin Braeckmans
Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging
published pages: 5975-5986, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.6b01411
Nano Letters 16/10 2019-06-06
2016 Ranhua Xiong, Sangram Keshari Samal, Jo Demeester, Andre G. Skirtach, Stefaan C. De Smedt, Kevin Braeckmans
Laser-assisted photoporation: fundamentals, technological advances and applications
published pages: 596-620, ISSN: 2374-6149, DOI: 10.1080/23746149.2016.1228476
Advances in Physics: X 1/4 2019-06-06
2017 Ranhua Xiong, Claire Drullion, Peter Verstraelen, Jo Demeester, Andre G. Skirtach, Corinne Abbadie, Winnok H. De Vos, Stefaan C. De Smedt, Kevin Braeckmans
Fast spatial-selective delivery into live cells
published pages: 198-204, ISSN: 0168-3659, DOI: 10.1016/J.JCONREL.2017.09.033
Journal of Controlled Release 266 2019-06-06
2018 Ranhua Xiong, Peter Verstraelen, Jo Demeester, Andre G. Skirtach, Jean-Pierre Timmermans, Stefaan C. De Smedt, Winnok H. De Vos, Kevin Braeckmans
Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation
published pages: Article 80, ISSN: 1662-5102, DOI: 10.3389/fncel.2018.00080
Frontiers in Cellular Neuroscience 12 2019-04-18
2018 Lotte Vermeulen, Juan Fraire, Laurens Raes, Ellen De Meester, Sarah De Keulenaer, Filip Van Nieuwerburgh, Stefaan De Smedt, Katrien Remaut, Kevin Braeckmans
Photothermally Triggered Endosomal Escape and Its Influence on Transfection Efficiency of Gold-Functionalized JetPEI/pDNA Nanoparticles
published pages: 2400, ISSN: 1422-0067, DOI: 10.3390/ijms19082400
International Journal of Molecular Sciences 19/8 2019-04-18

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