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Studying the bricks of microbial cities: characterization and structural properties of exopolysaccharides and their interaction with proteins and cations in anammox granular sludge

Total Cost €


EC-Contrib. €






 POLIS project word cloud

Explore the words cloud of the POLIS project. It provides you a very rough idea of what is the project "POLIS" about.

proteins    standardized    complete    ww    leadership    quality    tof    basic    training    removal    unrevealing    scientific    retention    innovative    provides    ir    biocorrosion    consuming    interactions    ms    sludge    significance    pyrolysis    rheological    benefit    lab    energy    gap    nitrogen    granule    bridge    aos    carbon    afm    perspectives    exopolysaccharides    redesign    extraction    uv    clsm    eps    closely    hplc    analytical    largely    mechanisms    acquired    granular    plant    mono    aeration    tb    techniques    multidisciplinary    anaerobic    modern    career    cations    maldi    bacteria    gc    loosely    anammox    yielding    treatment    microscopy    llct    dls    ammonium    isolated    60    fine    sls    microrheology    municipal    reactors    full    biofouling    light    visible    entire    definition    wastewater    combined    ft    saving    biofilm    structures    oxidation    nmr    scattering    tightly    100    efficient    slow    opening    ps    stability    lb    strengthen    bound    rate    excellence    divalent    organic    functional    area    researcher   

Project "POLIS" data sheet

The following table provides information about the project.


Organization address
city: MILANO
postcode: 20133

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Project website
 Total cost 168˙277 €
 EC max contribution 168˙277 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-12-01   to  2017-11-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    POLITECNICO DI MILANO IT (MILANO) coordinator 168˙277.00


 Project objective

The anaerobic ammonium oxidation (anammox) is an innovative technology for the removal of nitrogen from wastewater (ww) saving 60% of aeration and 100% of organic carbon. Its potential application to municipal ww allow a complete redesign of the present energy-consuming into an energy-yielding ww treatment plant. Due to the slow growth rate of anammox bacteria, their retention in the system, and therefore granule stability, is the main concern of this process. Granular stability is closely related to the properties of their exopolysaccharides (PS) and their interactions with proteins and mono-divalent cations. In the proposed research integrated analytical methods (UV-visible, FT-IR, MALDI-TOF MS, NMR, (pyrolysis)GC-MS, HPLC) will be used to investigate functional PS and their fine structures, unrevealing the mechanisms involved in anammox granular sludge formation and stability. Modern rheological (AOS, LLCT and Microrheology combined with CLSM), light scattering (DLS, SLS) and microscopy techniques (AFM, FT-IR, CLSM) will be used to investigate their interactions with proteins and cations. PS will be isolated from lab- and full-scale reactors. The definition of standardized extraction (loosely/tightly bound-EPS, LB/TB-EPS) and analytical methods will bridge the existing gap for efficient and meaningful comparison of the results from this and future research. The methods set up here, will largely contribute to increase the scientific significance of PS studies in anammox granular sludge area as well as facilitate the entire biofilm research field. Studies on biofouling and biocorrosion will also benefit from the high-quality of the information and basic knowledge acquired. This research will strengthen the EU leadership in anammox granular sludge technology also improving EU scientific excellence in PS research area and provides the researcher an advanced level of multidisciplinary training, opening concrete perspectives for his career development.


year authors and title journal last update
List of publications.
2017 Lotti T., Carretti E., Berti D., Lubello C., Malpei F.
Physicochemical characterization of EPS-based biomaterial recovered from anammox granular waste sludge
published pages: , ISSN: , DOI:
Proceedings of The IWA 2017 Conference on Sustainable Wastewater Treatment and Resource Recovery: Research, Planning, Design and Operation. Chongqing (China), 7-10 November 2017 2019-06-18
2017 T. Lotti, E. Carretti, D. Berti, C. Lubello, F. Malpei
Rheological Characterization of Biomaterial Recovered from Anammox Granular Sludge
published pages: 447-452, ISSN: , DOI:
Proceedings of Frontiers International Conference on Wastewater Treatment May 21-14, 2017, Palermo, ITALY 2019-06-18

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