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Studying the bricks of microbial cities: characterization and structural properties of exopolysaccharides and their interaction with proteins and cations in anammox granular sludge

Total Cost €


EC-Contrib. €






 POLIS project word cloud

Explore the words cloud of the POLIS project. It provides you a very rough idea of what is the project "POLIS" about.

treatment    pyrolysis    wastewater    cations    hplc    provides    extraction    sls    consuming    combined    bridge    tof    llct    eps    researcher    nmr    mono    organic    biocorrosion    tightly    mechanisms    yielding    career    bound    rate    aos    redesign    sludge    oxidation    lab    plant    60    maldi    microscopy    proteins    granular    strengthen    gap    bacteria    scattering    microrheology    entire    multidisciplinary    perspectives    loosely    anaerobic    efficient    acquired    lb    leadership    uv    ammonium    analytical    opening    municipal    stability    tb    granule    rheological    exopolysaccharides    biofouling    complete    retention    saving    dls    100    quality    unrevealing    ms    benefit    carbon    slow    removal    modern    excellence    aeration    functional    gc    scientific    area    visible    light    ft    isolated    ir    divalent    structures    biofilm    afm    full    ps    fine    significance    techniques    clsm    training    interactions    standardized    reactors    largely    innovative    definition    energy    basic    nitrogen    ww    anammox    closely   

Project "POLIS" data sheet

The following table provides information about the project.


Organization address
city: MILANO
postcode: 20133

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Project website
 Total cost 168˙277 €
 EC max contribution 168˙277 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-12-01   to  2017-11-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    POLITECNICO DI MILANO IT (MILANO) coordinator 168˙277.00


 Project objective

The anaerobic ammonium oxidation (anammox) is an innovative technology for the removal of nitrogen from wastewater (ww) saving 60% of aeration and 100% of organic carbon. Its potential application to municipal ww allow a complete redesign of the present energy-consuming into an energy-yielding ww treatment plant. Due to the slow growth rate of anammox bacteria, their retention in the system, and therefore granule stability, is the main concern of this process. Granular stability is closely related to the properties of their exopolysaccharides (PS) and their interactions with proteins and mono-divalent cations. In the proposed research integrated analytical methods (UV-visible, FT-IR, MALDI-TOF MS, NMR, (pyrolysis)GC-MS, HPLC) will be used to investigate functional PS and their fine structures, unrevealing the mechanisms involved in anammox granular sludge formation and stability. Modern rheological (AOS, LLCT and Microrheology combined with CLSM), light scattering (DLS, SLS) and microscopy techniques (AFM, FT-IR, CLSM) will be used to investigate their interactions with proteins and cations. PS will be isolated from lab- and full-scale reactors. The definition of standardized extraction (loosely/tightly bound-EPS, LB/TB-EPS) and analytical methods will bridge the existing gap for efficient and meaningful comparison of the results from this and future research. The methods set up here, will largely contribute to increase the scientific significance of PS studies in anammox granular sludge area as well as facilitate the entire biofilm research field. Studies on biofouling and biocorrosion will also benefit from the high-quality of the information and basic knowledge acquired. This research will strengthen the EU leadership in anammox granular sludge technology also improving EU scientific excellence in PS research area and provides the researcher an advanced level of multidisciplinary training, opening concrete perspectives for his career development.


year authors and title journal last update
List of publications.
2017 Lotti T., Carretti E., Berti D., Lubello C., Malpei F.
Physicochemical characterization of EPS-based biomaterial recovered from anammox granular waste sludge
published pages: , ISSN: , DOI:
Proceedings of The IWA 2017 Conference on Sustainable Wastewater Treatment and Resource Recovery: Research, Planning, Design and Operation. Chongqing (China), 7-10 November 2017 2019-06-18
2017 T. Lotti, E. Carretti, D. Berti, C. Lubello, F. Malpei
Rheological Characterization of Biomaterial Recovered from Anammox Granular Sludge
published pages: 447-452, ISSN: , DOI:
Proceedings of Frontiers International Conference on Wastewater Treatment May 21-14, 2017, Palermo, ITALY 2019-06-18

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