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LoveFood2Market

A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

LoveFood2Market project word cloud

Explore the words cloud of the LoveFood2Market project. It provides you a very rough idea of what is the project "LoveFood2Market" about.

criteria integrating scientists purification commitment pathogen innovative pathogenic commercialization escherichia bio bacillus chip time multiple micro card advancing coli meet biochip total entitled capture pathogens versatile food rapid industrial foodborne gathered meat single cartridge hours salmonella proven lab sensitive samples dairy detection prototype convergence lovefood credit modules nano technologists bacteria lysis chips thanks readiness dna amplification solution skilled size fourth capitalizes portable cereus fast platform enrichment hour performed listeria industry consumers perform successful extraction

Project "LoveFood2Market" data sheet

The following table provides information about the project.

Coordinator	IDRYMA TECHNOLOGIAS KAI EREVNAS Organization address address: N PLASTIRA STR 100 city: IRAKLEIO postcode: 70013 website: www.forth.gr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Greece [EL]
Project website	http://lovefood2market.eu
Total cost	3˙474˙732 €
EC max contribution	3˙151˙750 € (91%)
Programme	1. H2020-EU.2.1.1. (INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT))
Code Call	H2020-ICT-2015
Funding Scheme	IA
Starting year	2016
Duration (year-month-day)	from 2016-02-01 to 2019-04-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	IDRYMA TECHNOLOGIAS KAI EREVNAS IDRYMA TECHNOLOGIAS KAI EREVNAS Organization address address: N PLASTIRA STR 100 city: IRAKLEIO postcode: 70013 website: www.forth.gr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	EL (IRAKLEIO)	coordinator	809˙791.00
2	"NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS""" "NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS""" Organization address address: END OF PATRIARCHOU GRIGORIOU E AND 27 NEAPOLEOS STREET city: AGIA PARASKEVI postcode: 15341 website: www.demokritos.gr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	EL (AGIA PARASKEVI)	participant	994˙833.00
3	SENSEOR SAS SENSEOR SAS Organization address address: 505 ROUTE DES LUCIOLES LE NAVIGATOR BAT B PARC DU FONT DE L ORME LOT 3 city: VALBONNE postcode: 6560 website: www.senseor.com contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (VALBONNE)	participant	394˙875.00
4	INSTITUT PASTEUR INSTITUT PASTEUR Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 website: http://www.pasteur.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS CEDEX 15)	participant	340˙000.00
5	JOBST TECHNOLOGIES GMBH JOBST TECHNOLOGIES GMBH Organization address address: ENGESSERSTRASSE 4 B city: FREIBURG postcode: 79108 website: www.jobst-technologies.com contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (FREIBURG)	participant	316˙750.00
6	UNIVERZITA PARDUBICE UNIVERZITA PARDUBICE Organization address address: Studentska 95 city: PARDUBICE postcode: 532 10 website: www.uni-pardubice.eu contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	CZ (PARDUBICE)	participant	253˙500.00
7	MAKEDONIKI VIOMIHANIA GALAKTOS ANONIMOS ETERIA (MEVGAL SA) MAKEDONIKI VIOMIHANIA GALAKTOS ANONIMOS ETERIA (MEVGAL SA) Organization address address: KOYFALIA city: KOYFALIA, THESSALONIKI postcode: 57100 website: www.mevgal.gr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	EL (KOYFALIA, THESSALONIKI)	participant	42˙000.00

Map

Project objective

The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood.

These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment.

To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, Escherichia coli and Bacillus cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.

Deliverables

List of deliverables.
Report on SAW chip recycling capability	Documents, reports	2020-01-27 08:21:00
Environmental risk assessment associated with magnetic micro/nanoparticles applied for bioassays.	Documents, reports	2020-01-27 08:21:00
At least 3 high impact publications and / or review papers	Websites, patent fillings, videos etc.	2020-01-27 08:21:00
Integrated chips validated for detection of all targeted bacteria	Documents, reports	2020-01-27 08:21:00
Platforms encompassing 4 integrated chips fabricated and operated	Documents, reports	2020-01-27 08:21:00
Optimized method for acoustic signal enhancement after amplification using liposomes	Documents, reports	2020-01-27 08:21:00
ChipA fabricated and validated for detection of Salmonella.	Documents, reports	2020-01-27 08:21:00
Organization of workshops in international conferences on Food-Safety control	Websites, patent fillings, videos etc.	2020-01-27 08:21:00
Report on the shelf life of beads and the functionalized chips	Documents, reports	2020-01-27 08:21:00
Optimized acoustic detection protocols for 4 pathogens using the PCR or the RPA amplification method	Documents, reports	2020-01-27 08:20:59
Protocol and software for statistical analysis of bacteria sampling requirements	Documents, reports	2020-01-27 08:20:59
Optimized protocol for capturing beads carrying bacteria cells and cell lysis	Documents, reports	2020-01-27 08:20:59
ChipB fabricated and validated for detection of Salmonella. Report and chipB prototype.	Documents, reports	2020-01-27 08:20:59
Optimized protocol for short pre-culturing, isolation and concentration of bacteria from food using beads	Documents, reports	2020-01-27 08:20:59
Optimized PCR and RPA protocols for the efficient DNA amplification of 4 different pathogens	Documents, reports	2020-01-27 08:20:59
Optimized protocol for DNA purification	Documents, reports	2020-01-27 08:20:59
Bead trapping on chip. Report on design, fabrication, evaluation and chip prototype	Documents, reports	2020-01-27 08:20:59
Immunosorbents with desired specificity and binding capacity for bacteria cell capturing	Documents, reports	2020-01-27 08:20:59
Project web page established	Websites, patent fillings, videos etc.	2020-01-27 08:20:59

Take a look to the deliverables list in detail: detailed list of LoveFood2Market deliverables.

Publications

List of publications.
year	authors and title	journal	last update
2018	Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, Evangelos Gogolides Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays: Comparison with epoxy-silane coated glass slides published pages: 270-277, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2018.02.055	Colloids and Surfaces B: Biointerfaces 165	2020-01-27
2018	George Papadakis, Pavla Murasova, Audrey Hamiot, Katerina Tsougeni, Georgia Kaprou, Michael Eck, David Rabus, Zuzana Bilkova, Bruno Dupuy, Gerhard Jobst, Angeliki Tserepi, Evangelos Gogolides, Electra Gizeli Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples published pages: 52-58, ISSN: 0956-5663, DOI: 10.1016/j.bios.2018.03.056	Biosensors and Bioelectronics 111	2020-01-27
2018	Jana Srbova, Pavla Krulisova, Lucie Holubova, Iago Pereiro, Amel Bendali, Audrey Hamiot, Veronika Podzemna, Jan Macak, Bruno Dupuy, Stephanie Descroix, Jean-Louis Viovy, Zuzana Bilkova Advanced immunocapture of milk-borne Salmonella by microfluidic magnetically stabilized fluidized bed published pages: 526-533, ISSN: 0173-0835, DOI: 10.1002/elps.201700257	ELECTROPHORESIS 39/3	2020-01-27
2019	George Papadakis, Alexandros K. Pantazis, Maria Ntogka, Konstantinos Parasyris, Gesthimani-Ioanna Theodosi, Georgia Kaprou, Electra Gizeli 3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone published pages: 1329-1336, ISSN: 2379-3694, DOI: 10.1021/acssensors.9b00264	ACS Sensors 4/5	2020-01-27
2019	K. Tsougeni, A.S. Kastania, G.D. Kaprou, Michael Eck, Gerhard Jobst, P.S. Petrou, S.E. Kakabakos, D. Mastellos, E. Gogolides, A. Tserepi A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening published pages: 171-179, ISSN: 0925-4005, DOI: 10.1016/j.snb.2019.02.070	Sensors and Actuators B: Chemical 288	2020-01-27
2018	E. Cunaj, P.S. Petrou, G.D. Kaprou, S.E. Kakabakos, E. Gogolides, A. Tserepi Stable hydrophilization of FR4 and polyimide-based substrates implemented in microfluidics-on-PCB published pages: 292-299, ISSN: 0257-8972, DOI: 10.1016/j.surfcoat.2017.11.039	Surface and Coatings Technology 334	2020-01-27
2017	Athina S. Kastania, Katerina Tsougeni, Vassilios Constantoudis, Evangelos Gogolides Binding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments published pages: 247-257, ISSN: 0925-4005, DOI: 10.1016/j.snb.2017.06.113	Sensors and Actuators B: Chemical 253	2020-01-27
2017	G. Papadakis, J. M. Friedt, M. Eck, D. Rabus, G. Jobst, E. Gizeli Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics published pages: , ISSN: 1387-2176, DOI: 10.1007/s10544-017-0159-2	Biomedical Microdevices 19/3	2020-01-27
2017	Despina Moschou, Angeliki Tserepi The lab-on-PCB approach: tackling the Î¼TAS commercial upscaling bottleneck published pages: 1388-1405, ISSN: 1473-0197, DOI: 10.1039/c7lc00121e	Lab Chip 17/8	2020-01-27
2016	A Bourkoula, V Constantoudis, D Kontziampasis, P S Petrou, S E Kakabakos, A Tserepi, E Gogolides Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: the case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts published pages: 304002, ISSN: 0022-3727, DOI: 10.1088/0022-3727/49/30/304002	Journal of Physics D: Applied Physics 49/30	2020-01-27
2017	Panagiotis Dimitrakellis, Evangelos Gogolides, Angelos Zeniou, Kamil Awsiuk, Jakub Rysz, Mateusz M. Marzec Transition between stable hydrophilization and fast etching/hydrophilization of poly(methyl)methacrylate polymer using a novel atmospheric pressure dielectric barrier discharge source published pages: 41303, ISSN: 0734-2101, DOI: 10.1116/1.4984613	Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films 35/4	2020-01-27
2017	George Papadakis, Pasquale Palladino, Dimitra Chronaki, Achilleas Tsortos, Electra Gizeli Sample-to-answer acoustic detection of DNA in complex samples published pages: 8058-8061, ISSN: 1359-7345, DOI: 10.1039/c6cc10175e	Chem. Commun. 53/57	2020-01-27

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The information about "LOVEFOOD2MARKET" are provided by the European Opendata Portal: CORDIS opendata.