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LoveFood2Market

A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument

Total Cost €

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EC-Contrib. €

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Partnership

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Project "LoveFood2Market" data sheet

The following table provides information about the project.

Coordinator
IDRYMA TECHNOLOGIAS KAI EREVNAS 

Organization address
address: N PLASTIRA STR 100
city: IRAKLEIO
postcode: 70013
website: www.forth.gr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Greece [EL]
 Project website http://lovefood2market.eu
 Total cost 3˙474˙732 €
 EC max contribution 3˙151˙750 € (91%)
 Programme 1. H2020-EU.2.1.1. (INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT))
 Code Call H2020-ICT-2015
 Funding Scheme IA
 Starting year 2016
 Duration (year-month-day) from 2016-02-01   to  2019-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IDRYMA TECHNOLOGIAS KAI EREVNAS EL (IRAKLEIO) coordinator 809˙791.00
2    "NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS""" EL (AGIA PARASKEVI) participant 994˙833.00
3    SENSEOR SAS FR (VALBONNE) participant 394˙875.00
4    INSTITUT PASTEUR FR (PARIS CEDEX 15) participant 340˙000.00
5    JOBST TECHNOLOGIES GMBH DE (FREIBURG) participant 316˙750.00
6    UNIVERZITA PARDUBICE CZ (PARDUBICE) participant 253˙500.00
7    MAKEDONIKI VIOMIHANIA GALAKTOS ANONIMOS ETERIA (MEVGAL SA) EL (KOYFALIA, THESSALONIKI) participant 42˙000.00

Map

 Project objective

The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood.

These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment.

To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, Escherichia coli and Bacillus cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.

 Deliverables

List of deliverables.
Report on SAW chip recycling capability Documents, reports 2020-01-27 08:21:00
Environmental risk assessment associated with magnetic micro/nanoparticles applied for bioassays. Documents, reports 2020-01-27 08:21:00
At least 3 high impact publications and / or review papers Websites, patent fillings, videos etc. 2020-01-27 08:21:00
Integrated chips validated for detection of all targeted bacteria Documents, reports 2020-01-27 08:21:00
Platforms encompassing 4 integrated chips fabricated and operated Documents, reports 2020-01-27 08:21:00
Optimized method for acoustic signal enhancement after amplification using liposomes Documents, reports 2020-01-27 08:21:00
ChipA fabricated and validated for detection of Salmonella. Documents, reports 2020-01-27 08:21:00
Organization of workshops in international conferences on Food-Safety control Websites, patent fillings, videos etc. 2020-01-27 08:21:00
Report on the shelf life of beads and the functionalized chips Documents, reports 2020-01-27 08:21:00
Optimized acoustic detection protocols for 4 pathogens using the PCR or the RPA amplification method Documents, reports 2020-01-27 08:20:59
Protocol and software for statistical analysis of bacteria sampling requirements Documents, reports 2020-01-27 08:20:59
Optimized protocol for capturing beads carrying bacteria cells and cell lysis Documents, reports 2020-01-27 08:20:59
ChipB fabricated and validated for detection of Salmonella. Report and chipB prototype. Documents, reports 2020-01-27 08:20:59
Optimized protocol for short pre-culturing, isolation and concentration of bacteria from food using beads Documents, reports 2020-01-27 08:20:59
Optimized PCR and RPA protocols for the efficient DNA amplification of 4 different pathogens Documents, reports 2020-01-27 08:20:59
Optimized protocol for DNA purification Documents, reports 2020-01-27 08:20:59
Bead trapping on chip. Report on design, fabrication, evaluation and chip prototype Documents, reports 2020-01-27 08:20:59
Immunosorbents with desired specificity and binding capacity for bacteria cell capturing Documents, reports 2020-01-27 08:20:59
Project web page established Websites, patent fillings, videos etc. 2020-01-27 08:20:59

Take a look to the deliverables list in detail:  detailed list of LoveFood2Market deliverables.

 Publications

year authors and title journal last update
List of publications.
2018 Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, Evangelos Gogolides
Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays: Comparison with epoxy-silane coated glass slides
published pages: 270-277, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2018.02.055
Colloids and Surfaces B: Biointerfaces 165 2020-01-27
2018 George Papadakis, Pavla Murasova, Audrey Hamiot, Katerina Tsougeni, Georgia Kaprou, Michael Eck, David Rabus, Zuzana Bilkova, Bruno Dupuy, Gerhard Jobst, Angeliki Tserepi, Evangelos Gogolides, Electra Gizeli
Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples
published pages: 52-58, ISSN: 0956-5663, DOI: 10.1016/j.bios.2018.03.056
Biosensors and Bioelectronics 111 2020-01-27
2018 Jana Srbova, Pavla Krulisova, Lucie Holubova, Iago Pereiro, Amel Bendali, Audrey Hamiot, Veronika Podzemna, Jan Macak, Bruno Dupuy, Stephanie Descroix, Jean-Louis Viovy, Zuzana Bilkova
Advanced immunocapture of milk-borne Salmonella by microfluidic magnetically stabilized fluidized bed
published pages: 526-533, ISSN: 0173-0835, DOI: 10.1002/elps.201700257
ELECTROPHORESIS 39/3 2020-01-27
2019 George Papadakis, Alexandros K. Pantazis, Maria Ntogka, Konstantinos Parasyris, Gesthimani-Ioanna Theodosi, Georgia Kaprou, Electra Gizeli
3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone
published pages: 1329-1336, ISSN: 2379-3694, DOI: 10.1021/acssensors.9b00264
ACS Sensors 4/5 2020-01-27
2019 K. Tsougeni, A.S. Kastania, G.D. Kaprou, Michael Eck, Gerhard Jobst, P.S. Petrou, S.E. Kakabakos, D. Mastellos, E. Gogolides, A. Tserepi
A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening
published pages: 171-179, ISSN: 0925-4005, DOI: 10.1016/j.snb.2019.02.070
Sensors and Actuators B: Chemical 288 2020-01-27
2018 E. Cunaj, P.S. Petrou, G.D. Kaprou, S.E. Kakabakos, E. Gogolides, A. Tserepi
Stable hydrophilization of FR4 and polyimide-based substrates implemented in microfluidics-on-PCB
published pages: 292-299, ISSN: 0257-8972, DOI: 10.1016/j.surfcoat.2017.11.039
Surface and Coatings Technology 334 2020-01-27
2017 Athina S. Kastania, Katerina Tsougeni, Vassilios Constantoudis, Evangelos Gogolides
Binding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments
published pages: 247-257, ISSN: 0925-4005, DOI: 10.1016/j.snb.2017.06.113
Sensors and Actuators B: Chemical 253 2020-01-27
2017 G. Papadakis, J. M. Friedt, M. Eck, D. Rabus, G. Jobst, E. Gizeli
Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics
published pages: , ISSN: 1387-2176, DOI: 10.1007/s10544-017-0159-2
Biomedical Microdevices 19/3 2020-01-27
2017 Despina Moschou, Angeliki Tserepi
The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck
published pages: 1388-1405, ISSN: 1473-0197, DOI: 10.1039/c7lc00121e
Lab Chip 17/8 2020-01-27
2016 A Bourkoula, V Constantoudis, D Kontziampasis, P S Petrou, S E Kakabakos, A Tserepi, E Gogolides
Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: the case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts
published pages: 304002, ISSN: 0022-3727, DOI: 10.1088/0022-3727/49/30/304002
Journal of Physics D: Applied Physics 49/30 2020-01-27
2017 Panagiotis Dimitrakellis, Evangelos Gogolides, Angelos Zeniou, Kamil Awsiuk, Jakub Rysz, Mateusz M. Marzec
Transition between stable hydrophilization and fast etching/hydrophilization of poly(methyl)methacrylate polymer using a novel atmospheric pressure dielectric barrier discharge source
published pages: 41303, ISSN: 0734-2101, DOI: 10.1116/1.4984613
Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films 35/4 2020-01-27
2017 George Papadakis, Pasquale Palladino, Dimitra Chronaki, Achilleas Tsortos, Electra Gizeli
Sample-to-answer acoustic detection of DNA in complex samples
published pages: 8058-8061, ISSN: 1359-7345, DOI: 10.1039/c6cc10175e
Chem. Commun. 53/57 2020-01-27

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