Opendata, web and dolomites

transLEISHion SIGNED

A targeted knockout screen for identification of Leishmania membrane transporters required for infection of macrophages

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 transLEISHion project word cloud

Explore the words cloud of the transLEISHion project. It provides you a very rough idea of what is the project "transLEISHion" about.

knockout    leishmanias    infection    pathogen    parasitic    sand    unexplored    hundreds    survival    macrophages    mutants    editing    drug    despite    membrane    forms    subvert    form    formulations    immune    viability    urging    hitherto    functional    phagolysosome    modern    manipulation    leishmaniases    gene    proliferates    anti    mediated    bite    fly    transcriptomic    surface    crispr    attack    throughput    identification    extensively    transmitted    cas9    macrophage    transporters    generation    first    disease    world    mexicana    promastigote    largely    rapid    host    cheaper    kinetoplastida    amastigote    48    overexpressed    allowed    acute    molecular    leishmania    nutrient    cell    interactions    intracellular    safer    intrinsic    drawbacks    amastigotes    agent    diseases    time    biology    functions    differentiation    it    encoding    genes    cellular    expressed    systematic    efforts    causative    parasite    harsh    proteins    mechanisms    defence    drugs    thanks    treatment    library    genome    unclear    roles    upregulated    uncharacterized    proposes    peptidases   

Project "transLEISHion" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 195˙454.00

Map

 Project objective

Leishmaniases are among the world's most neglected diseases. It´s causative agent - Leishmania - is transmitted by the bite of a sand fly. In the host, the parasite is taken up by macrophages and proliferates in the harsh conditions of the host phagolysosome. Currently available drugs for treatment present major drawbacks, urging the need for more effective, safer, and cheaper drugs. The molecular and cellular mechanisms that enable Leishmania to subvert macrophage defence mechanisms and cause disease remain largely unclear. Thanks to modern comparative transcriptomic analysis, the identification of hundreds of genes overexpressed in intracellular amastigote forms was possible for the first time and identified cell surface proteins, transporters, peptidases and many uncharacterized genes with possible roles in host-pathogen interactions. Only recently, a high-throughput gene editing method based on CRISPR-Cas9 allowed for the first time easy manipulation of Leishmanias´ complex genome and rapid functional analysis of genes. For over 50 years membrane transporters have been extensively studied in kinetoplastida, due to their role in the intrinsic parasite nutrient needs, defence from host immune attack and uptake of drug formulations. Despite these efforts a large number of such transporters expressed in amastigotes remain uncharacterized. The present project proposes (1) the generation of a library of 48 L. mexicana mutants by systematic CRISPR-Cas9 mediated knockout of amastigote-upregulated genes encoding membrane transporters, (2) Identification of membrane transporters required for viability of the promastigote parasite form and the (3) Identification of membrane transporters required for differentiation into amastigotes, macrophage infection, intracellular survival. The results of this project are expected to provide new insight into functions of hitherto unexplored aspects of amastigote biology and identify potential new targets for anti-parasitic drugs.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TRANSLEISHION" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "TRANSLEISHION" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Photonic Radar (2019)

Implementation of Long Reach Hybrid Photonic Radar System and convergence over FSO and PON Networks

Read More  

CORRELATION (2020)

Characterization and prediction of service-level traffic for future sliced mobile network

Read More  

STOPFIRE (2019)

Emergency Decision Support System of Offshore Platform Fires

Read More