Explore the words cloud of the Glycoli project. It provides you a very rough idea of what is the project "Glycoli" about.
The following table provides information about the project.
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
|Coordinator Country||Switzerland [CH]|
|Total cost||187˙419 €|
|EC max contribution||187˙419 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2016-11-01 to 2018-10-31|
Take a look of project's partnership.
|1||EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH||CH (ZUERICH)||coordinator||187˙419.00|
Metabolic engineering of E. coli has been highly successful in producing diverse free oligosaccharides for research and commercial applications. Currently, we are poised to address the next major challenge: the site-specific synthesis of oligosaccharides directly onto proteins in the E. coli cytosol. In this project we aim at the metabolic engineering of an N-glycosylation pathway in the E. coli cytosol using heterologous glycosyltransferases (GTs) to produce defined glycoprotein structures. Starting point is the newly discovered family of cytosolic N-glycosyltransferases (NGT), which transfer a single glucose residue onto proteins at asparagine (N) residues in the N-X-S/T sequon. Co-expression of an NGT with a galactosyltransferase has been shown to yield N-linked lactose in the E. coli cytosol. This N-linked lactose is an ideal starting point for the design of cytosolic N-glycosylation pathways to synthesize important glycans on proteins. Two goals are delineated. 1) Bacterial GTs will be screened, and potentially engineered, for their ability to extend the N-lactose with defined sugars, thereby generating a diverse repertoire of glycans directly synthesized on proteins. The focus will be on screening of fucosyltransferases and efficient production of fucosylated oligosaccharides, as these are central glycan epitopes in diverse physiological processes. 2) The newly developed glycosylation pathways will be applied to a range of protein substrates in order to explore and address possible limitations of the glycosylation system. The result will be a well-characterized glycoengineering toolbox enabling the bottom-up production of defined glycoprotein structures in E. coli.
|year||authors and title||journal||last update|
Hanne L.P. Tytgat
Franklin L. Nobrega
John van der Oost
Willem M. de Vos
Bowel Biofilms: Tipping Points between a Healthy and Compromised Gut?
published pages: , ISSN: 0966-842X, DOI: 10.3929/ethz-b-000304638
|Trends in Microbiology||2019-04-18|
Sonja BÃ¤umel, Hanne L. P. Tytgat, Birgit Nemec, Ruth Schmidt, Loo Wee Chia, Hauke Smidt
Fifty Percent Human - how art brings us in touch with our microbial cohabitants
published pages: 571-574, ISSN: 1751-7915, DOI: 10.1111/1751-7915.13285
|Microbial Biotechnology 11/4||2019-03-20|
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "GLYCOLI" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (email@example.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "GLYCOLI" are provided by the European Opendata Portal: CORDIS opendata.