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Characterization of a novel mechanism restricting virus infection in reproductive tissues

Total Cost €


EC-Contrib. €






 RESTRIVIR project word cloud

Explore the words cloud of the RESTRIVIR project. It provides you a very rough idea of what is the project "RESTRIVIR" about.

gfp    ems    like    drosophila    decipher    differentially    expressing    insects    shrna    transposon    expression    health    expressed    vivo    delta    borne    sirna    mammals    permissive    sequencing    tested    combination    reproductive    potent    detected    followed    genome    mutants    line    interference    screens    derepression    restricting    somatic    uncover    tissues    transformed    stress    flock    genes    mutagenesis    replication    reverse    rnai    responsible    cells    mutant    virus    infected    host    restrictive    identification    fcs    mechanism    monolayer    laboratory    protect    lines    melanogaster    viral    antiviral    gene    follicular    replicon    origin    sort    tract    b2    human    scientific    worldwide    controls    genetic    ex    provides    except    restriction    mobilization    partial    fhv    acts    characterization    defense    cell    pirna    innate    derepressed    resequencing    candidate    completely    oss    function    pirnas    rna    immunity    fc    defenses    implications    surrounding    viruses    germline    house    arthropod    screening    fruitfly   

Project "RESTRIVIR" data sheet

The following table provides information about the project.


Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Like mammals, insects are infected by many viruses. Among them, arthropod-borne viruses are an increasing worldwide health concern. Insects have potent innate antiviral defenses, of which RNA interference (RNAi) is the main and best studied. In the fruitfly Drosophila melanogaster, the siRNA pathway controls viral replication in somatic tissues. The piRNA pathway, another RNAi based response, acts specifically in the reproductive tract (germline and follicular cells (FC), a monolayer of somatic cells surrounding the germline), to protect the genome against transposon mobilization. Other innate immunity or stress pathways also contribute to the antiviral defense. The host laboratory obtained evidence for a new mechanism controlling viral replication in the FCs of Drosophila. Indeed, a viral replicon derived from Flock House Virus and expressing GFP (FHVΔB2-GFP) is completely derepressed in somatic tissues of mutants for the siRNA pathway, except in FCs, where derepression is partial. No piRNAs of viral origin can be detected in these cells. This viral replicon provides a unique system to decipher a novel pathway restricting viral replication. For this, I will use a combination of forward and reverse genetic screens. EMS mutagenesis, screening for GFP expression and genome resequencing will be used to uncover genes responsible for restricting the replicon in FCs. Next, I will use GFP expression to sort restrictive and permissive FCs in a siRNA pathway mutant followed by RNA sequencing to identify differentially expressed genes. The function of the identified genes will be tested in vivo, using shRNA Drosophila lines. Finally, OSS cells, an FC-derived cell line, will be transformed with the FHVΔB2-GFP replicon to evaluate candidate gene function ex vivo. The identification and characterization of genes involved in a novel viral restriction pathway will increase the knowledge about innate antiviral immunity, an important scientific topic with human health implications.

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The information about "RESTRIVIR" are provided by the European Opendata Portal: CORDIS opendata.

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