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RESTRIVIR SIGNED

Characterization of a novel mechanism restricting virus infection in reproductive tissues

Total Cost €

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EC-Contrib. €

0

Partnership

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 RESTRIVIR project word cloud

Explore the words cloud of the RESTRIVIR project. It provides you a very rough idea of what is the project "RESTRIVIR" about.

genes    melanogaster    shrna    transposon    tissues    reproductive    interference    mammals    replicon    defenses    viral    mobilization    mechanism    flock    provides    pirnas    arthropod    viruses    uncover    fc    transformed    germline    cell    mutants    delta    lines    like    expressing    somatic    resequencing    scientific    human    detected    laboratory    screens    fhv    sequencing    stress    house    differentially    defense    replication    protect    gfp    followed    antiviral    restriction    virus    decipher    function    except    mutant    implications    gene    infected    tested    origin    partial    expression    responsible    b2    restrictive    drosophila    monolayer    completely    pirna    tract    borne    mutagenesis    line    permissive    potent    cells    characterization    ex    oss    genetic    identification    host    rna    follicular    candidate    sirna    reverse    surrounding    fcs    acts    sort    genome    expressed    vivo    health    immunity    derepressed    rnai    restricting    ems    controls    insects    combination    derepression    fruitfly    screening    worldwide    innate   

Project "RESTRIVIR" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://ibmc-m3i.cnrs.fr/en/research-groups/antiviral-immunity/
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Like mammals, insects are infected by many viruses. Among them, arthropod-borne viruses are an increasing worldwide health concern. Insects have potent innate antiviral defenses, of which RNA interference (RNAi) is the main and best studied. In the fruitfly Drosophila melanogaster, the siRNA pathway controls viral replication in somatic tissues. The piRNA pathway, another RNAi based response, acts specifically in the reproductive tract (germline and follicular cells (FC), a monolayer of somatic cells surrounding the germline), to protect the genome against transposon mobilization. Other innate immunity or stress pathways also contribute to the antiviral defense. The host laboratory obtained evidence for a new mechanism controlling viral replication in the FCs of Drosophila. Indeed, a viral replicon derived from Flock House Virus and expressing GFP (FHVΔB2-GFP) is completely derepressed in somatic tissues of mutants for the siRNA pathway, except in FCs, where derepression is partial. No piRNAs of viral origin can be detected in these cells. This viral replicon provides a unique system to decipher a novel pathway restricting viral replication. For this, I will use a combination of forward and reverse genetic screens. EMS mutagenesis, screening for GFP expression and genome resequencing will be used to uncover genes responsible for restricting the replicon in FCs. Next, I will use GFP expression to sort restrictive and permissive FCs in a siRNA pathway mutant followed by RNA sequencing to identify differentially expressed genes. The function of the identified genes will be tested in vivo, using shRNA Drosophila lines. Finally, OSS cells, an FC-derived cell line, will be transformed with the FHVΔB2-GFP replicon to evaluate candidate gene function ex vivo. The identification and characterization of genes involved in a novel viral restriction pathway will increase the knowledge about innate antiviral immunity, an important scientific topic with human health implications.

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