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RESTRIVIR SIGNED

Characterization of a novel mechanism restricting virus infection in reproductive tissues

Total Cost €

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EC-Contrib. €

0

Partnership

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 RESTRIVIR project word cloud

Explore the words cloud of the RESTRIVIR project. It provides you a very rough idea of what is the project "RESTRIVIR" about.

replication    cells    acts    arthropod    germline    borne    mutants    viruses    worldwide    defenses    derepressed    restricting    line    expression    antiviral    genetic    scientific    like    interference    completely    screens    partial    rna    vivo    fc    expressed    protect    pirnas    human    immunity    pirna    transformed    characterization    ex    except    follicular    fcs    resequencing    genome    mechanism    mammals    reproductive    oss    mobilization    differentially    combination    surrounding    host    sort    potent    defense    gene    sequencing    identification    b2    health    tested    detected    uncover    ems    transposon    monolayer    implications    followed    fruitfly    reverse    viral    rnai    sirna    fhv    lines    screening    tract    cell    restrictive    permissive    gfp    origin    stress    melanogaster    decipher    controls    shrna    delta    function    provides    virus    laboratory    flock    derepression    house    restriction    innate    mutant    candidate    genes    drosophila    mutagenesis    somatic    insects    tissues    replicon    infected    expressing    responsible   

Project "RESTRIVIR" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://ibmc-m3i.cnrs.fr/en/research-groups/antiviral-immunity/
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Like mammals, insects are infected by many viruses. Among them, arthropod-borne viruses are an increasing worldwide health concern. Insects have potent innate antiviral defenses, of which RNA interference (RNAi) is the main and best studied. In the fruitfly Drosophila melanogaster, the siRNA pathway controls viral replication in somatic tissues. The piRNA pathway, another RNAi based response, acts specifically in the reproductive tract (germline and follicular cells (FC), a monolayer of somatic cells surrounding the germline), to protect the genome against transposon mobilization. Other innate immunity or stress pathways also contribute to the antiviral defense. The host laboratory obtained evidence for a new mechanism controlling viral replication in the FCs of Drosophila. Indeed, a viral replicon derived from Flock House Virus and expressing GFP (FHVΔB2-GFP) is completely derepressed in somatic tissues of mutants for the siRNA pathway, except in FCs, where derepression is partial. No piRNAs of viral origin can be detected in these cells. This viral replicon provides a unique system to decipher a novel pathway restricting viral replication. For this, I will use a combination of forward and reverse genetic screens. EMS mutagenesis, screening for GFP expression and genome resequencing will be used to uncover genes responsible for restricting the replicon in FCs. Next, I will use GFP expression to sort restrictive and permissive FCs in a siRNA pathway mutant followed by RNA sequencing to identify differentially expressed genes. The function of the identified genes will be tested in vivo, using shRNA Drosophila lines. Finally, OSS cells, an FC-derived cell line, will be transformed with the FHVΔB2-GFP replicon to evaluate candidate gene function ex vivo. The identification and characterization of genes involved in a novel viral restriction pathway will increase the knowledge about innate antiviral immunity, an important scientific topic with human health implications.

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