Explore the words cloud of the Cells-in-drops project. It provides you a very rough idea of what is the project "Cells-in-drops" about.
The following table provides information about the project.
|Coordinator Country||Lithuania [LT]|
|Total cost||130˙779 €|
|EC max contribution||130˙779 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2016-03-21 to 2018-03-20|
Take a look of project's partnership.
|1||VILNIAUS UNIVERSITETAS||LT (VILNIUS)||coordinator||130˙779.00|
Tackling heterogeneous cell populations at single-cell resolution is becoming increasingly important in different branches of biology and biomedicine. Many useful techniques have been developed to profile and even selectively purify single-cells, however, the demand for techniques with better analytical performance and improved high-throughput capabilities, remains very high. Droplet microfluidics can fulfill this demand by bringing higher throughput, scalability and single molecule resolution that are hard to achieve with conventional technologies. In this project, a droplet microfluidics platform will be developed and applied for ultra-high-throughput single-cell screening and sequencing. The project will be focused on B-cells that produce therapeutic antibodies or biomolecules of industrial interest. Cell compartmentalization into microfluidic droplets together with capture beads and barcoded DNA primers will enable a direct establishment of the linkage between the genotype (genes or mRNA) and phenotype (binding, regulatory or activity of secreted proteins). The proposed work will allow the quantitative high-throughput antibody phenotyping without loosing the original heavy-light chain pairing, a significant advantage over other technologies. Like no other system available to-date this the technological approach outlined in this proposal will provide a unique way to identify the primary sequence of heavy and light IgG genes encoding functional monoclonal antibodies directly from single-cells, without a need to perform gene cloning or cell immortalization. The results of this work are likely to bring a significant impact not only in applied biological sciences but also in biotechnology and biomedicine.
|year||authors and title||journal||last update|
Rapolas Zilionis, Juozas Nainys, Adrian Veres, Virginia Savova, David Zemmour, Allon M Klein, Linas Mazutis
Single-cell barcoding and sequencing using droplet microfluidics
published pages: 44-73, ISSN: 1754-2189, DOI: 10.1038/nprot.2016.154
|Nature Protocols 12/1||2019-07-26|
Greta Zubaite, Karolis Simutis, Robertas Galinis, Valdemaras Milkus, Vaidotas Kiseliovas, Linas Mazutis
Droplet Microfluidics Approach for Single-DNA Molecule Amplification and Condensation into DNA-Magnesium-Pyrophosphate Particles
published pages: 62, ISSN: 2072-666X, DOI: 10.3390/mi8020062
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