Explore the words cloud of the PERSIST project. It provides you a very rough idea of what is the project "PERSIST" about.
The following table provides information about the project.
|Coordinator Country||Denmark [DK]|
|Total cost||212˙194 €|
|EC max contribution||212˙194 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2016-04-01 to 2018-03-31|
Take a look of project's partnership.
|1||KOBENHAVNS UNIVERSITET||DK (KOBENHAVN)||coordinator||212˙194.00|
As a global healthcare issue, chronic and recurrent infections are often caused by multidrug tolerant persister cells, which are regulated by redundant pathways involving the almost ubiquitous alarmone (p)ppGpp. Despite extensive studies, two major questions in the persistence field remain to be answered, 1) the extent to which (p)ppGpp is involved in persistence and 2) how persistent cells resuscitate. To address these questions, three state-of-the-art techniques will be used in this project. First, DRaCALA (differential radial capillary action of ligand assay) will be used to systematically screen for proteins directly binding (p)ppGpp for the first time. The potential fellow from the Imperial College London has tremendous experience in DRaCALA. Second, barcode analysis by sequencing (Bar-seq) technique will be used to screen for persistence-specific and, for the first time, also resuscitation-specific genes. Collaboration with the partner Professor Duncan Maskel at the University of Cambridge, UK, a specialist in quantitative DNA deep sequencing, will ensure smooth progress of this objective and enable the potential fellow to be trained with this first-class technique. Both DRaCALA and Bar-seq will be transferred to the host lab. At last, identified candidate genes and proteins through the first two objectives will be validated with the contemporary single cell persistence assay developed in the host Professor Kenn Gerdes’s lab, at the University of Copenhagen, Denmark. Bar-seq and single cell persistence assay will be transferred to the potential fellow by senior postdocs in the partner and host labs, respectively. The tripartite cooperation with complementary expertise on a timely important project is expected to generate significant amount of high-quality data for the persistence field, facilitate extensive transfer of knowledge and promote the potential fellow to obtain an independent research position in the area of molecular microbiology and physiology.
|year||authors and title||journal||last update|
Yong Zhang, Eva ZbornÃkovÃ¡, Dominik Rejman, Kenn Gerdes
Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli
published pages: e02188-17, ISSN: 2150-7511, DOI: 10.1128/mBio.02188-17
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "PERSIST" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (email@example.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "PERSIST" are provided by the European Opendata Portal: CORDIS opendata.
Narrative, Writing, and the Teotihuacan Language: Exploring Language History Through Phylogenetics, Epigraphy and IconographyRead More
Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. ElegansRead More
Increasing the scope of CO2-utilising photoreactions: asymmetric photosynthesis of amino acidsRead More