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Systematic identification of (p)ppGpp-dependent multidrug and stress tolerance factors

Total Cost €


EC-Contrib. €






 PERSIST project word cloud

Explore the words cloud of the PERSIST project. It provides you a very rough idea of what is the project "PERSIST" about.

professor    candidate    questions    differential    cambridge    data    dracala    maskel    gerdes    despite    first    london    postdocs    answered    redundant    deep    time    specialist    host    regulated    tripartite    trained    smooth    amount    bar    cell    position    denmark    copenhagen    last    labs    healthcare    cooperation    almost    single    kenn    quality    as    ligand    proteins    persister    expertise    technique    caused    capillary    persistent    systematically    tremendous    radial    physiology    chronic    assay    techniques    tolerant    quantitative    dna    complementary    resuscitation    microbiology    barcode    lab    progress    respectively    contemporary    alarmone    independent    ubiquitous    recurrent    issue    generate    class    genes    extensive    imperial    university    area    senior    multidrug    obtain    transfer    validated    action    global    screen    resuscitate    duncan    fellow    molecular    persistence    college    infections    ppgpp    uk    transferred    sequencing    binding    seq    cells   

Project "PERSIST" data sheet

The following table provides information about the project.


Organization address
address: NORREGADE 10
postcode: 1165

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Project website
 Total cost 212˙194 €
 EC max contribution 212˙194 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KOBENHAVNS UNIVERSITET DK (KOBENHAVN) coordinator 212˙194.00


 Project objective

As a global healthcare issue, chronic and recurrent infections are often caused by multidrug tolerant persister cells, which are regulated by redundant pathways involving the almost ubiquitous alarmone (p)ppGpp. Despite extensive studies, two major questions in the persistence field remain to be answered, 1) the extent to which (p)ppGpp is involved in persistence and 2) how persistent cells resuscitate. To address these questions, three state-of-the-art techniques will be used in this project. First, DRaCALA (differential radial capillary action of ligand assay) will be used to systematically screen for proteins directly binding (p)ppGpp for the first time. The potential fellow from the Imperial College London has tremendous experience in DRaCALA. Second, barcode analysis by sequencing (Bar-seq) technique will be used to screen for persistence-specific and, for the first time, also resuscitation-specific genes. Collaboration with the partner Professor Duncan Maskel at the University of Cambridge, UK, a specialist in quantitative DNA deep sequencing, will ensure smooth progress of this objective and enable the potential fellow to be trained with this first-class technique. Both DRaCALA and Bar-seq will be transferred to the host lab. At last, identified candidate genes and proteins through the first two objectives will be validated with the contemporary single cell persistence assay developed in the host Professor Kenn Gerdes’s lab, at the University of Copenhagen, Denmark. Bar-seq and single cell persistence assay will be transferred to the potential fellow by senior postdocs in the partner and host labs, respectively. The tripartite cooperation with complementary expertise on a timely important project is expected to generate significant amount of high-quality data for the persistence field, facilitate extensive transfer of knowledge and promote the potential fellow to obtain an independent research position in the area of molecular microbiology and physiology.


year authors and title journal last update
List of publications.
2018 Yong Zhang, Eva Zborníková, Dominik Rejman, Kenn Gerdes
Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli
published pages: e02188-17, ISSN: 2150-7511, DOI: 10.1128/mBio.02188-17
mBio 9/2 2019-06-13

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The information about "PERSIST" are provided by the European Opendata Portal: CORDIS opendata.

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