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GolgiBiosSys SIGNED

Identification and characterisation of plant Golgi biosynthetic multiprotein systems

Total Cost €


EC-Contrib. €






 GolgiBiosSys project word cloud

Explore the words cloud of the GolgiBiosSys project. It provides you a very rough idea of what is the project "GolgiBiosSys" about.

precise    thought    secondly    functional    yield    localisation    complexes    docking    functions    biomaterial    map    least    proteome    fibre    knockout    carbohydrate    secretion    conjunction    proteins    fuel    arrangement    multiprotein    material    composition    interaction    skills    separation    independent    golgi    gain    sub    proteomic    recalcitrant    accomplishment    cell    little    cooperative    lipid    humanity    biosphere    organelle    biochemical    academic    expertise    data    validated    organisation    structures    experimental    bioenergy    polysaccharides    carbon    biosynthetic    biosynthesis    quantitative    food    glycosylation    governed    industries    leadership    model    membrane    myself    orchestrated    organisational    pivotal    amount    earth    relevance    throughput    stoichiometries    renewable    presented    researcher    measured    lines    performed    molecular    organic    interactions    thirdly    action    technique    firstly    protein    immunoprecipitation    abundant    auxiliary    performs    apparatus    assignment    insights    enzymes    deepen   

Project "GolgiBiosSys" data sheet

The following table provides information about the project.


Organization address
address: Raemistrasse 101
postcode: 8092

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 187˙419 €
 EC max contribution 187˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-05-01   to  2018-04-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

The Golgi apparatus is a system of organised membrane structures that performs pivotal cell functions: biosynthesis and secretion of polysaccharides, protein and lipid glycosylation. The relevance of this organelle is measured by the amount of biomaterial produced by its activity. The highly abundant polysaccharides account for at least a third of all renewable organic carbon on Earth and are of great importance to the biosphere and to humanity, providing food, fibre and fuel. The functionality of the Golgi is thought to be governed by molecular organisational features, as the precise arrangement of its membrane proteins. Each of the numerous biosynthetic pathways performed in the Golgi requires the cooperative and orchestrated action and interaction of several different enzymes and auxiliary proteins. Currently very little is known about the composition and sub-Golgi localisation of such multiprotein systems. Firstly, a comprehensive insight into the interaction map of the Golgi proteome is needed. I propose to develop and apply a technique that will allow high-throughput assignment of membrane protein interactions, and identify stoichiometries. An experimental design is presented based on separation of membrane protein complexes in conjunction with state of the art quantitative proteomic approaches. Secondly, the results will be validated on selected multiprotein systems by immunoprecipitation and functional biochemical studies on knockout lines. Thirdly, the data will be used to model the interactions by protein-protein docking. The accomplishment of this project will provide new insights into the organisation and activity of the biosynthetic systems. Such insight is of great value to the production of specific polysaccharides, tailored to yield less recalcitrant material for the bioenergy and carbohydrate industries. Through this work I aim to deepen my academic expertise, gain technical and leadership skills and to establish myself as an independent researcher.

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The information about "GOLGIBIOSSYS" are provided by the European Opendata Portal: CORDIS opendata.

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