Explore the words cloud of the Mechan-of-Chromo project. It provides you a very rough idea of what is the project "Mechan-of-Chromo" about.
The following table provides information about the project.
AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
|Coordinator Country||Spain [ES]|
|Total cost||1˙894˙999 €|
|EC max contribution||1˙894˙999 € (100%)|
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
|Duration (year-month-day)||from 2016-06-01 to 2021-05-31|
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|1||AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS||ES (MADRID)||coordinator||1˙894˙999.00|
The global folding of the chromosome is mediated by Structural Maintenance of Chromosome (SMC) proteins, which stabilize the higher-order chromatin architecture by bringing distant DNA sequences together. Despite over a decade of work on these systems, their mechanism remains unknown, largely because of difficulty in re-capitulating physiological DNA binding and condensation in vitro. Moreover, traditional biochemical approaches are poorly suited for the study of processes that are fundamentally mechanical in nature. However, key breakthroughs, including the discovery that SMC is loaded by Spo0J protein at parS sites in vivo, and that parS sites act as global condensation centres for the chromosome have opened new possibilities to study chromosome organisation using single-molecule (SM) approaches. Importantly, our recent experiments with Magnetic Tweezers (MT) have already revealed a novel function of Spo0J in condensing DNA via a parS-independent binding mechanism.
Inspired by these recent discoveries, I have devised a series of novel SM biophysical approaches with the ambitious goal of determining the mechanism of action of SMC complexes, including understanding the role of SMC loaders and SMC accessory subunits, and how these proteins are regulated by ATP binding and hydrolysis for chromosome organisation. The rationale behind this approach is that SM methods are particularly well-suited for monitoring DNA cohesion and condensation where manipulation of individual DNA molecules, measurement of forces, and addition of proteins and buffer solutions can be carefully controlled. High throughput MT will be combined with fast video imaging, optical trapping, and fluorescence; and will be used to interrogate hypothetical models for SMC-DNA interactions. Finally, the novel assays developed here may be applicable to other protein-DNA interactions including variant SMC-like proteins specialized for other biological functions such as DNA repair.
|year||authors and title||journal||last update|
Alberto Marin-Gonzalez, J.â€‰G. Vilhena, Fernando Moreno-Herrero, Ruben Perez
DNA Crookedness Regulates DNA Mechanical Properties at Short Length Scales
published pages: , ISSN: 0031-9007, DOI: 10.1103/physrevlett.122.048102
|Physical Review Letters 122/4||2019-09-04|
Julene Madariaga-Marcos, Cesar L Pastrana, Gemma LM Fisher, Mark Simon Dillingham, Fernando Moreno-Herrero
ParB dynamics and the critical role of the CTD in DNA condensation unveiled by combined force-fluorescence measurements
published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.43812
R. Arroyo, A. MartÃn-GonzÃ¡lez, M. Echaide, A. Jain, W.H. Brondyk, J. Rosenbaum, F. Moreno-Herrero, J. PÃ©rez-Gil
Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D
published pages: 1495-1509, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2018.03.027
|Journal of Molecular Biology 430/10||2019-05-09|
BÃ¡rbara MartÃn-GarcÃa, Alejandro MartÃn-GonzÃ¡lez, Carolina Carrasco, Ana M HernÃ¡ndez-Arriaga, RubÃ©n RuÃz-Quero, RamÃ³n DÃaz-Orejas, Clara Aicart-Ramos, Fernando Moreno-Herrero, MarÃa A Oliva
The TubRâ€“centromere complex adopts a double-ring segrosome structure in Type III partition systems
published pages: 5704-5716, ISSN: 0305-1048, DOI: 10.1093/nar/gky370
|Nucleic Acids Research 46/11||2019-05-09|
Oliver J Wilkinson, Alejandro MartÃn-GonzÃ¡lez, Haejoo Kang, Sarah J Northall, Dale B Wigley, Fernando Moreno-Herrero, Mark Simon Dillingham
CtIP forms a tetrameric dumbbell-shaped particle which bridges complex DNA end structures for double-strand break repair
published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.42129
Alberto Marin-Gonzalez, J. G. Vilhena, Ruben Perez, Fernando Moreno-Herrero
Understanding the mechanical response of double-stranded DNA and RNA under constant stretching forces using all-atom molecular dynamics
published pages: 7049-7054, ISSN: 0027-8424, DOI: 10.1073/pnas.1705642114
|Proceedings of the National Academy of Sciences 114/27||2019-06-18|
Gemma LM Fisher, CÃ©sar L Pastrana, Victoria A Higman, Alan Koh, James A Taylor, Annika Butterer, Timothy Craggs, Frank Sobott, Heath Murray, Matthew P Crump, Fernando Moreno-Herrero, Mark S Dillingham
The structural basis for dynamic DNA binding and bridging interactions which condense the bacterial centromere
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.28086
J. Madariaga-Marcos, S. HormeÃ±o, C. L. Pastrana, G. L. M. Fisher, M. S. Dillingham, F. Moreno-Herrero
Force determination in lateral magnetic tweezers combined with TIRF microscopy
published pages: 4579-4590, ISSN: 2040-3364, DOI: 10.1039/c7nr07344e
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