Explore the words cloud of the SPLICE3D project. It provides you a very rough idea of what is the project "SPLICE3D" about.
The following table provides information about the project.
Coordinator |
UNITED KINGDOM RESEARCH AND INNOVATION
There are not information about this coordinator. Please contact Fabio for more information, thanks. |
Coordinator Country | United Kingdom [UK] |
Project website | https://www2.mrc-lmb.cam.ac.uk/groups/nagai/ |
Total cost | 1˙069˙584 € |
EC max contribution | 1˙069˙584 € (100%) |
Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
Code Call | ERC-2015-AdG |
Funding Scheme | ERC-ADG |
Starting year | 2016 |
Duration (year-month-day) | from 2016-06-01 to 2020-09-30 |
Take a look of project's partnership.
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1 | UNITED KINGDOM RESEARCH AND INNOVATION | UK (SWINDON) | coordinator | 1˙069˙584.00 |
2 | MEDICAL RESEARCH COUNCIL | UK (SWINDON) | coordinator | 0.00 |
The protein coding sequences of the majority of eukaryotic genes are interrupted by non-coding introns. The spliceosome is an immense and intricate molecular machine that catalyses the excision of introns from pre-mRNAs and splicing together of exons to produce mature mRNA. This is a crucial process in eukaryotic gene expression and we aims to greatly increase our understanding of its molecular mechanism through cryoEM studies of the spliceosome. The spliceosome comprises five canonical subunits, namely U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles (snRNPs) and numerous non-snRNP factors. During the initial stages, U1 and U2 snRNPs bind the 5’-splice site and branch point of pre-mRNA, respectively, and the spliceosome is fully assembled when the U4/U6.U5 tri-snRNP is recruited. The spliceosome then becomes activated through extensive structural and compositional remodeling which leads to the formation of the catalytic RNA core. Recently we determined the crystal structure of U1 snRNP and two key spliceosomal proteins: Prp8, which harbours the catalytic RNA core and Brr2, which catalyses the rearrangement of the RNA interaction network and plays a crucial role in spliceosomal activation. We also solved the structure of U4/U6.U5 tri-snRNP by cryoEM and revealed the nearly complete organisation of U5 snRNA and U4/U6 snRNA and over 30 proteins within this complex, providing crucial insights into the activation mechanism and the active site of the spliceosome. Building on these achievements we aim to determine the structure of the entire spliceosome stalled at different steps of the splicing reaction so that the molecular mechanism of pre-mRNA splicing is understood in structural terms. This will enormously increase our understanding of this fundamental process of eukaryotic gene expression and such knowledge will clarify the molecular pathology of diseases caused by mutations in spliceosomal components and may facilitate therapeutic intervention.
year | authors and title | journal | last update |
---|---|---|---|
2018 |
Clemens Plaschka, Pei-Chun Lin, Clément Charenton, Kiyoshi Nagai Prespliceosome structure provides insights into spliceosome assembly and regulation published pages: 419-422, ISSN: 0028-0836, DOI: 10.1038/s41586-018-0323-8 |
Nature 559/7714 | 2020-01-15 |
2017 |
Sebastian M. Fica, Chris Oubridge, Wojciech P. Galej, Max E. Wilkinson, Xiao-Chen Bai, Andrew J. Newman, Kiyoshi Nagai Structure of a spliceosome remodelled for exon ligation published pages: 377-380, ISSN: 0028-0836, DOI: 10.1038/nature21078 |
Nature 542/7641 | 2020-01-15 |
2019 |
Clément Charenton, Max E. Wilkinson, Kiyoshi Nagai Mechanism of 5′ splice site transfer for human spliceosome activation published pages: eaax3289, ISSN: 0036-8075, DOI: 10.1126/science.aax3289 |
Science | 2020-01-15 |
2019 |
Sebastian M. Fica, Chris Oubridge, Max E. Wilkinson, Andrew J. Newman, Kiyoshi Nagai A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation published pages: eaaw5569, ISSN: 0036-8075, DOI: 10.1126/science.aaw5569 |
Science | 2020-01-15 |
2019 |
Clemens Plaschka, Andrew J. Newman, Kiyoshi Nagai Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast published pages: a032391, ISSN: 1943-0264, DOI: 10.1101/cshperspect.a032391 |
Cold Spring Harbor Perspectives in Biology | 2020-01-15 |
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The information about "SPLICE3D" are provided by the European Opendata Portal: CORDIS opendata.
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