Opendata, web and dolomites

CHASSY SIGNED

Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

Project "CHASSY" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK 

Organization address
address: WESTERN ROAD
city: Cork
postcode: T12 YN60
website: www.ucc.ie

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Ireland [IE]
 Project website http://www.chassy.eu
 Total cost 5˙634˙610 €
 EC max contribution 5˙634˙610 € (100%)
 Programme 1. H2020-EU.2.1.4. (INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies – Biotechnology)
 Code Call H2020-NMBP-BIO-2016
 Funding Scheme RIA
 Starting year 2016
 Duration (year-month-day) from 2016-12-01   to  2020-11-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK IE (Cork) coordinator 1˙216˙232.00
2    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) participant 950˙091.00
3    INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT FR (PARIS CEDEX 07) participant 747˙595.00
4    BIOPETROLIA AB SE (GOTEBORG) participant 702˙200.00
5    CHALMERS TEKNISKA HOEGSKOLA AB SE (GOETEBORG) participant 652˙135.00
6    JOHANN WOLFGANG GOETHE-UNIVERSITATFRANKFURT AM MAIN DE (FRANKFURT AM MAIN) participant 535˙500.00
7    CLARIANT PRODUKTE (DEUTSCHLAND) GMBH DE (FRANKFURT AM MAIN) participant 367˙356.00
8    NOVA-INSTITUT FUR POLITISCHE UND OKOLOGISCHE INNOVATION GMBH DE (Huerth) participant 251˙000.00
9    UCC ACADEMY DESIGNATED ACTIVITY COMPANY IE (Cork) participant 212˙500.00
10    EVOLVA AG CH (REINACH) participant 0.00

Map

 Project objective

CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.

 Deliverables

List of deliverables.
Library of branded publishable information (leaflets, posters, booklets, slide-set, etc.) Documents, reports 2020-04-24 01:26:27
The CHASSY public access website up and running Websites, patent fillings, videos etc. 2020-04-24 01:26:27
Technological fact sheets on CHASSY strains Documents, reports 2020-04-24 01:26:28

Take a look to the deliverables list in detail:  detailed list of CHASSY deliverables.

 Publications

year authors and title journal last update
List of publications.
2020 Macarena Larroude, Heykel Trabelsi, Jean-Marc Nicaud, Tristan Rossignol
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity
published pages: , ISSN: 0141-5492, DOI: 10.1007/s10529-020-02805-4
Biotechnology Letters 2020-04-24
2020 Florian Wernig, Eckhard Boles, Mislav Oreb
De novo biosynthesis of 8-hydroxyoctanoic acid via a medium-chain length specific fatty acid synthase and cytochrome P450 in Saccharomyces cerevisiae
published pages: e00111, ISSN: 2214-0301, DOI: 10.1016/j.mec.2019.e00111
Metabolic Engineering Communications 10 2020-04-24
2019 Javier A. Varela, Martina Puricelli, Raúl A. Ortiz-Merino, Romina Giacomobono, Stephanie Braun-Galleani, Kenneth H. Wolfe, John P. Morrissey
Origin of Lactose Fermentation in Kluyveromyces lactis by Interspecies Transfer of a Neo-functionalized Gene Cluster during Domestication
published pages: , ISSN: 0960-9822, DOI: 10.1016/j.cub.2019.10.044
Current Biology 2020-04-24
2018 Yu, Tao; Zhou, Yongjin J.; Wenning, Leonie; Liu, Quanli; Krivoruchko, Anastasia; Siewers, Verena; Nielsen, Jens; David, Florian
Author Correction: Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
published pages: , ISSN: 2041-1723, DOI: 10.1038/ncomms16220
Nature Communications 1 2020-04-24
2019 Else-Jasmijn Hassing, Philip A. de Groot, Vita R. Marquenie, Jack T. Pronk, Jean-Marc G. Daran
Connecting central carbon and aromatic amino acid metabolisms to improve de novo 2-phenylethanol production in Saccharomyces cerevisiae
published pages: 165-180, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2019.09.011
Metabolic Engineering 56 2020-04-24
2019 Macarena Larroude, Young‐Kyoung Park, Paul Soudier, Monika Kubiak, Jean‐Marc Nicaud, Tristan Rossignol
A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology
published pages: , ISSN: 1751-7915, DOI: 10.1111/1751-7915.13427
Microbial Biotechnology 2020-04-24
2018 Yating Hu, Zhiwei Zhu, Jens Nielsen, Verena Siewers
Heterologous transporter expression for improved fatty alcohol secretion in yeast
published pages: 51-58, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2017.11.008
Metabolic Engineering 45 2020-04-24
2017 Michal A. Świat, Sofia Dashko, Maxime den Ridder, Melanie Wijsman, John van der Oost, Jean-Marc Daran, Pascale Daran-Lapujade
FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae
published pages: 12585-12598, ISSN: 0305-1048, DOI: 10.1093/nar/gkx1007
Nucleic Acids Research 45/21 2020-04-24
2019 Arun S. Rajkumar, Javier A. Varela, Hannes Juergens, Jean-Marc G. Daran, John P. Morrissey
Biological Parts for Kluyveromyces marxianus Synthetic Biology
published pages: , ISSN: 2296-4185, DOI: 10.3389/fbioe.2019.00097
Frontiers in Bioengineering and Biotechnology 7 2020-04-24
2019 Javier A. Varela, Martina Puricelli, Noemi Montini, John P. Morrissey
Expansion and Diversification of MFS Transporters in Kluyveromyces marxianus
published pages: , ISSN: 1664-302X, DOI: 10.3389/fmicb.2018.03330
Frontiers in Microbiology 9 2020-04-24
2018 Leonie Baumann, Arun S. Rajkumar, John P. Morrissey, Eckhard Boles, Mislav Oreb
A Yeast-Based Biosensor for Screening of Short- and Medium-Chain Fatty Acid Production
published pages: , ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00309
ACS Synthetic Biology 2020-04-24
2018 M. Larroude, T. Rossignol, J.-M. Nicaud, R. Ledesma-Amaro
Synthetic biology tools for engineering Yarrowia lipolytica
published pages: , ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2018.10.004
Biotechnology Advances 2020-04-24
2018 Yongjin J. Zhou, Eduard J. Kerkhoven, Jens Nielsen
Barriers and opportunities in bio-based production of hydrocarbons
published pages: , ISSN: 2058-7546, DOI: 10.1038/s41560-018-0197-x
Nature Energy 2020-04-24
2017 Tao Yu, Yongjin J. Zhou, Leonie Wenning, Quanli Liu, Anastasia Krivoruchko, Verena Siewers, Jens Nielsen, Florian David
Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
published pages: 15587, ISSN: 2041-1723, DOI: 10.1038/ncomms15587
Nature Communications 8 2020-04-24
2018 Robert Mans, Jean-Marc G Daran, Jack T Pronk
Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production
published pages: 47-56, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2017.10.011
Current Opinion in Biotechnology 50 2020-04-24
2018 Robert Mans, Jean-Marc G Daran, Jack T Pronk
Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production
published pages: 47-56, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2017.10.011
Current Opinion in Biotechnology 50 2020-04-24
2017 Benjamín J Sánchez, Cheng Zhang, Avlant Nilsson, Petri‐Jaan Lahtvee, Eduard J Kerkhoven, Jens Nielsen
Improving the phenotype predictions of a yeast genome‐scale metabolic model by incorporating enzymatic constraints
published pages: 935, ISSN: 1744-4292, DOI: 10.15252/msb.20167411
Molecular Systems Biology 13/8 2020-04-24
2019 Hongzhong Lu, Feiran Li, Benjamín J. Sánchez, Zhengming Zhu, Gang Li, Iván Domenzain, Simonas Marcišauskas, Petre Mihail Anton, Dimitra Lappa, Christian Lieven, Moritz Emanuel Beber, Nikolaus Sonnenschein, Eduard J. Kerkhoven, Jens Nielsen
A consensus S. cerevisiae metabolic model Yeast8 and its ecosystem for comprehensively probing cellular metabolism
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-11581-3
Nature Communications 10/1 2020-04-24

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "CHASSY" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "CHASSY" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.2.1.4.)

SHIKIFACTORY100 (2019)

Modular cell factories for the production of 100 compounds from the shikimate pathway

Read More  

BioRoboost (2018)

Fostering Synthetic Biology standardisation through international collaboration

Read More  

SynBio4Flav (2019)

Synthetic microbial consortia-based platform for flavonoids production using synthetic biology

Read More