Opendata, web and dolomites

Mushroomics SIGNED

Functional genomics in Schizophyllum commune: leveraging the diversity in this hypervariable fungus to understand mushroom development

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

Project "Mushroomics" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITEIT UTRECHT 

Organization address
address: HEIDELBERGLAAN 8
city: UTRECHT
postcode: 3584 CS
website: www.uu.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 1˙500˙000 €
 EC max contribution 1˙500˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2017
 Duration (year-month-day) from 2017-01-01   to  2021-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITEIT UTRECHT NL (UTRECHT) coordinator 1˙500˙000.00

Map

 Project objective

Mushrooms are the sexual reproductive structures of basidiomycetes. Much remains to be learned about the molecular regulation of mushroom development. Only a few genes have been implicated in this process, but their exact function remains unknown. Schizophyllum commune is a model system for mushroom-forming fungi. It was recently shown that this species shows the highest intraspecies DNA variability in eukaryotes with 150 SNPs per 1000 base pairs, yet they can still reproduce sexually. This hypervariabity is also illustrated by variability in the response to environmental factors that initiate mushroom development and by the differences in morphology within this species. I propose a functional genomics approach to elucidate the molecular genetics of mushroom development. We have a collection of 100 S. commune strains sampled world-wide. I propose to leverage the extraordinary diversity among these strains. I will identify genes that explain differences in mushroom morphology and in responses to environmental stimuli such as light and CO2. The genes/alleles responsible for these phenotypes will by mapped by genome sequencing followed by bulk segregant analysis. Transcription factors (TFs) are expected to play an important role in regulation of mushroom development. Therefore I propose to systematically study TF function by generating a knock-down collection of all predicted 313 TFs. The phenotypes of the resulting strains will be analyzed and target genes of TFs will be identified by RNA-Seq and ChIP-Seq. The genes identified by these strategies will subsequently be analyzed to determine their function. My initial results demonstrate the feasibility of this approach. The availability of the strain collection, the available molecular toolbox, and my strong expertise both in genomics and molecular biology of mushroom development will enable me to take a huge step forward in our understanding of mushroom development.

 Publications

year authors and title journal last update
List of publications.
2019 Krisztina Krizsán, Éva Almási, Zsolt Merényi, Neha Sahu, Máté Virágh, Tamás Kószó, Stephen Mondo, Brigitta Kiss, Balázs Bálint, Ursula Kües, Kerrie Barry, Judit Cseklye, Botond Hegedüs, Bernard Henrissat, Jenifer Johnson, Anna Lipzen, Robin A. Ohm, István Nagy, Jasmyn Pangilinan, Juying Yan, Yi Xiong, Igor V. Grigoriev, David S. Hibbett, László G. Nagy
Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi
published pages: 7409-7418, ISSN: 0027-8424, DOI: 10.1073/pnas.1817822116
Proceedings of the National Academy of Sciences 116/15 2019-11-08
2019 Peter Jan Vonk, Natalia Escobar, Han A. B. Wösten, Luis G. Lugones, Robin A. Ohm
High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-019-44133-2
Scientific Reports 9/1 2019-11-08
2018 Peter Jan Vonk, Robin A. Ohm
The role of homeodomain transcription factors in fungal development
published pages: , ISSN: 1749-4613, DOI: 10.1016/j.fbr.2018.04.002
Fungal Biology Reviews 2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "MUSHROOMICS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "MUSHROOMICS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

PROGRESS (2019)

The Enemy of the Good: Towards a Theory of Moral Progress

Read More  

VictPart (2019)

Righting Victim Participation in Transitional Justice

Read More  

DISINTEGRATION (2019)

The Mass Politics of Disintegration

Read More