Opendata, web and dolomites

DNA NANO MAP SIGNED

Affordable DNA Mapping using Solidstate-Nanopores

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 DNA NANO MAP project word cloud

Explore the words cloud of the DNA NANO MAP project. It provides you a very rough idea of what is the project "DNA NANO MAP" about.

extracted    clinical    resolution    gene    match    stranded    microbial    molecules    dyes    composition    natural    channel    membrane    technique    species    drop    genomic    identification    time    methyltransferases    provides    expensive    region    living    dna    sequencer    sequencing    detect    easily    nanopores    idea    consist    amplification    analogues    translocates    molecule    tool    mapping    transfer    consuming    small    recognize    heralded    map    basic    ph    microscopy    basepairs    super    methionine    subsequent    notoriously    underlying    amount    maps    requirement    cleanroom    pattern    sequence    double    fluorescence    facilities    machine    adenosyl    biomolecular    suggest    pcr    too    pore    pores    gut    rdna    fluorescent    attached    dominant    mass    synthetic    trace    induce    labels    subsequently    solid    cheap    few    label    bias    cofactor    alternative    16s    converted    limited    fraction    primer    susceptible    setting   

Project "DNA NANO MAP" data sheet

The following table provides information about the project.

Coordinator
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE 

Organization address
address: BATIMENT CE 3316 STATION 1
city: LAUSANNE
postcode: 1015
website: www.epfl.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Project website https://lben.epfl.ch/page-79460-en-html/
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-04-01   to  2019-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE CH (LAUSANNE) coordinator 175˙419.00

Map

 Project objective

DNA analysis will become an important clinical tool in identification the composition of microbial species living in the gut. The most dominant technique at the time is based on PCR amplification of the 16s rDNA gene and subsequent sequencing of the variable region in the gene. This method however is limited in the amount of detail it provides and is notoriously susceptible to primer bias. Furthermore, due to the requirement of a PCR machine and sequencer the technique is too expensive and time consuming for a clinical setting.

Over the past few years nanopores have been heralded as a cheap alternative for biomolecular analysis. Specifically solid-state nanopores, which consist of a small pore in a solid membrane, have the potential for cost-effective mass-production in cleanroom facilities. Furthermore, since these pores can detect double stranded DNA they can easily be used for analysis of DNA maps.

In this proposal I suggest to use DNA mapping with solid state nanopores. The basic idea is to sequence-specifically label DNA molecules using DNA Methyltransferases and synthetic analogues of the natural cofactor S-adenosyl-methionine. The pattern of labels attached to the DNA is unique for the underlying sequence.

In my Ph.D. I used this method to transfer fluorescent dyes to the DNA and subsequently extracted the DNA map using super resolution fluorescence microscopy. In this case, the attached label will induce a current-drop when the DNA molecule translocates through the channel. The extracted current-trace will then be converted to a DNA map in basepairs and can be used to match to a real sequence. This proposed method can be used to identify genomic elements and eventually be used to recognize species, for a fraction of the cost of 16s rDNA sequencing.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "DNA NANO MAP" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "DNA NANO MAP" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MathematicsAnalogies (2019)

Mathematics Analogies

Read More  

PNAIC (2018)

Positive and Negative Asymmetry in Intergroup Contact: Its Impact on Linguistic Forms of Communication and Physiological Responses

Read More  

MIGPSC (2018)

Shaping the European Migration Policy: the role of the security industry

Read More