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NascenTomiX SIGNED

Ribosome inhibition by nascent or antimicrobial peptides

Total Cost €

0

EC-Contrib. €

0

Partnership

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 NascenTomiX project word cloud

Explore the words cloud of the NascenTomiX project. It provides you a very rough idea of what is the project "NascenTomiX" about.

chain    inhibit    consolidator    generation    sensors    natural    diversity    form    arrested    translational    point    position    rely    unexplored    details    act    technique    ribosome    stalling    expression    precise    amino    designing    dependent    protein    eukaryotes    handle    regulators    insights    fundamental    complementary    genetic    molecular    primarily    weight    gene    synthesis    sequence    ribosomal    erc    acid    explaining    antibiotics    inverse    function    interacting    characterization    largely    tunnel    mediated    occasionally    mrna    bases    during    perform    depends    group    nature    types    decipher    trans    region    structural    peptides    code    governing    molecules    yielded    metabolite    arrest    exit    translation    small    ligand    inhibitory    regulation    entire    molecule    sense    naturally    toeprinting    precisely    peptide    action    biochemical    nascent    bacteria    sensing    groundbreaking    mode    subunit    maps    occurring    retaining    coding   

Project "NascenTomiX" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE 

Organization address
address: RUE DE TOLBIAC 101
city: PARIS
postcode: 75654
website: www.inserm.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙999˙125 €
 EC max contribution 1˙999˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-06-01   to  2022-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE FR (PARIS) coordinator 1˙999˙125.00

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 Project objective

During the translation of genetic information into protein by the ribosome, nascent peptides occasionally inhibit their own synthesis by interacting with the exit tunnel of the large ribosomal subunit. Known as nascent chain-mediated translational arrest, this process depends primarily upon the amino acid sequence of the arrest peptide. However, it can also rely upon the sensing of a low molecular weight ligand by the ribosome nascent chain complex, explaining its use for metabolite-dependent gene regulation in both bacteria and eukaryotes. Biochemical and structural studies of arrest peptides have yielded key insights into their mode of action, but their ability to sense different types of small molecules, their impact as regulators of gene expression in nature and the precise molecular details behind the arrest process are still largely unexplored. The groundbreaking aim of this ERC Consolidator research program is to decipher the arrest code governing nascent chain-mediated translational arrest in bacteria. My approach will be based on a technique recently developed in my group, referred to here as inverse toeprinting, which precisely maps the position of an arrested ribosome nascent chain complex on the mRNA while retaining the entire peptide-coding region up to the point of stalling.

The overall aim will be achieved through four complementary objectives: (i) to assess the extent to which arrest peptides can act as small molecule sensors; (ii) to identify naturally occurring arrest peptides in bacteria; (iii) to develop trans-inhibitory peptides that target the ribosome; and (iv) to perform the structural characterization of new ribosome inhibitory peptides.

By addressing the natural diversity and molecular bases of the arrest process, this project will be the key to understanding a unique form of gene regulation and a fundamental aspect of ribosome function. It will also provide a handle for designing next-generation antibiotics.

 Publications

year authors and title journal last update
List of publications.
2018 Justine Charon, Aitor Manteca, C. Axel Innis
Using the Bacterial Ribosome as a Discovery Platform for Peptide-Based Antibiotics
published pages: 75-84, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00927
Biochemistry 58/2 2020-02-06
2018 Mario Mardirossian, Natacha Pérébaskine, Monica Benincasa, Stefano Gambato, Sven Hofmann, Paul Huter, Claudia Müller, Kai Hilpert, C. Axel Innis, Alessandro Tossi, Daniel N. Wilson
The Dolphin Proline-Rich Antimicrobial Peptide Tur1A Inhibits Protein Synthesis by Targeting the Bacterial Ribosome
published pages: 530-539.e7, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2018.02.004
Cell Chemical Biology 25/5 2019-05-23
2018 Britta Seip, Guénaël Sacheau, Denis Dupuy, C Axel Innis
Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA libraries
published pages: e201800148, ISSN: 2575-1077, DOI: 10.26508/lsa.201800148
Life Science Alliance 1/5 2019-05-23

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