Opendata, web and dolomites

NascenTomiX SIGNED

Ribosome inhibition by nascent or antimicrobial peptides

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NascenTomiX project word cloud

Explore the words cloud of the NascenTomiX project. It provides you a very rough idea of what is the project "NascenTomiX" about.

arrest    form    yielded    fundamental    code    nascent    tunnel    regulators    synthesis    metabolite    sense    diversity    handle    stalling    molecular    arrested    gene    primarily    mode    antibiotics    ribosomal    subunit    designing    mediated    amino    bacteria    characterization    governing    peptides    sensing    explaining    translational    biochemical    inverse    molecule    molecules    small    dependent    act    inhibitory    weight    translation    interacting    generation    rely    precise    naturally    groundbreaking    inhibit    bases    trans    during    protein    depends    details    occurring    coding    precisely    types    complementary    structural    occasionally    natural    largely    eukaryotes    group    chain    toeprinting    retaining    peptide    action    technique    consolidator    perform    maps    genetic    regulation    function    unexplored    position    region    sensors    exit    decipher    acid    erc    entire    sequence    ligand    insights    ribosome    point    mrna    expression    nature   

Project "NascenTomiX" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE 

Organization address
address: RUE DE TOLBIAC 101
city: PARIS
postcode: 75654
website: www.inserm.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙999˙125 €
 EC max contribution 1˙999˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-06-01   to  2022-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE FR (PARIS) coordinator 1˙999˙125.00

Map

 Project objective

During the translation of genetic information into protein by the ribosome, nascent peptides occasionally inhibit their own synthesis by interacting with the exit tunnel of the large ribosomal subunit. Known as nascent chain-mediated translational arrest, this process depends primarily upon the amino acid sequence of the arrest peptide. However, it can also rely upon the sensing of a low molecular weight ligand by the ribosome nascent chain complex, explaining its use for metabolite-dependent gene regulation in both bacteria and eukaryotes. Biochemical and structural studies of arrest peptides have yielded key insights into their mode of action, but their ability to sense different types of small molecules, their impact as regulators of gene expression in nature and the precise molecular details behind the arrest process are still largely unexplored. The groundbreaking aim of this ERC Consolidator research program is to decipher the arrest code governing nascent chain-mediated translational arrest in bacteria. My approach will be based on a technique recently developed in my group, referred to here as inverse toeprinting, which precisely maps the position of an arrested ribosome nascent chain complex on the mRNA while retaining the entire peptide-coding region up to the point of stalling.

The overall aim will be achieved through four complementary objectives: (i) to assess the extent to which arrest peptides can act as small molecule sensors; (ii) to identify naturally occurring arrest peptides in bacteria; (iii) to develop trans-inhibitory peptides that target the ribosome; and (iv) to perform the structural characterization of new ribosome inhibitory peptides.

By addressing the natural diversity and molecular bases of the arrest process, this project will be the key to understanding a unique form of gene regulation and a fundamental aspect of ribosome function. It will also provide a handle for designing next-generation antibiotics.

 Publications

year authors and title journal last update
List of publications.
2018 Justine Charon, Aitor Manteca, C. Axel Innis
Using the Bacterial Ribosome as a Discovery Platform for Peptide-Based Antibiotics
published pages: 75-84, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00927
Biochemistry 58/2 2020-02-06
2018 Mario Mardirossian, Natacha Pérébaskine, Monica Benincasa, Stefano Gambato, Sven Hofmann, Paul Huter, Claudia Müller, Kai Hilpert, C. Axel Innis, Alessandro Tossi, Daniel N. Wilson
The Dolphin Proline-Rich Antimicrobial Peptide Tur1A Inhibits Protein Synthesis by Targeting the Bacterial Ribosome
published pages: 530-539.e7, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2018.02.004
Cell Chemical Biology 25/5 2019-05-23
2018 Britta Seip, Guénaël Sacheau, Denis Dupuy, C Axel Innis
Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA libraries
published pages: e201800148, ISSN: 2575-1077, DOI: 10.26508/lsa.201800148
Life Science Alliance 1/5 2019-05-23

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NASCENTOMIX" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NASCENTOMIX" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

evolSingleCellGRN (2019)

Constraint, Adaptation, and Heterogeneity: Genomic and single-cell approaches to understanding the evolution of developmental gene regulatory networks

Read More  

IMMUNOTHROMBOSIS (2019)

Cross-talk between platelets and immunity - implications for host homeostasis and defense

Read More  

RODRESET (2019)

Development of novel optogenetic approaches for improving vision in macular degeneration

Read More