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FLUOSWITCH SIGNED

Pushing the frontiers of biological imaging with genetically encoded fluorescence switches

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

FLUOSWITCH project word cloud

Explore the words cloud of the FLUOSWITCH project. It provides you a very rough idea of what is the project "FLUOSWITCH" about.

optics innovative super push observe breakthroughs continuous revolutionized integrator acids extraordinary acting quantitatively dynamics decade activated switches cell workings genetically levels orthogonal cells act activation proposes molecules living complexity tools sequential benefited interaction fluorescence detectors comprehensively clinical labeling final permanent visualization transiently detection boundaries class nucleic cellular probes imaging revealing multiplexing light first circuits microscopy molecular time frontiers switching intracellular basic advancing resolution off classes dynamic inner last tissues active biomolecules implementing images fluorogenic biology network techniques back led endogenous organisms multiplexed modalities action biological discoveries encoded proteins interrogate reveal

Project "FLUOSWITCH" data sheet

The following table provides information about the project.

Coordinator	SORBONNE UNIVERSITE Organization address address: 21 RUE DE L'ECOLE DE MEDECINE city: PARIS postcode: 75006 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Total cost	2˙000˙000 €
EC max contribution	2˙000˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2016-COG
Funding Scheme	ERC-COG
Starting year	2017
Duration (year-month-day)	from 2017-05-01 to 2022-04-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	SORBONNE UNIVERSITE SORBONNE UNIVERSITE Organization address address: 21 RUE DE L'ECOLE DE MEDECINE city: PARIS postcode: 75006 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	851˙562.00
2	ECOLE NORMALE SUPERIEURE ECOLE NORMALE SUPERIEURE Organization address address: 45, RUE D'ULM city: PARIS CEDEX 05 postcode: 75230 website: http://www.ens.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS CEDEX 05)	participant	1˙148˙437.00

Map

Project objective

Biological imaging is essential for revealing the inner workings of living systems. Among the numerous imaging modalities, light microscopy has revolutionized biological research. In addition to advances in optics and detectors, imaging has benefited from the development of molecular tools to observe biomolecules in action. Although the last decade’s breakthroughs in imaging have led to new discoveries in biology, there are still extraordinary opportunities for basic and clinical research in further advancing imaging capabilities. This project proposes to develop new classes of probes to advance biological imaging and allow the study of biological processes in all their complexity. First, I propose to push the boundaries of multiplexing and super-resolution imaging in living cells developing a new class of fluorogenic probes that act as genetically encoded fluorescence on/off switches. Highly multiplexed images will be built up over sequential activation of orthogonal fluorescence on/off switches, while continuous switching will allow implementing innovative dynamic super-resolution techniques in living cells. Then, I will develop dynamic fluorescence on/off switches enabling to reveal the dynamics of intracellular processes, focusing in particular on the visualization of interaction dynamics in real-time, and the dynamic detection of endogenous molecules (e.g. proteins, nucleic acids) in living cells. The final part will be dedicated to the development of probes acting as molecular integrator switches to identify active cell circuits in whole tissues or organisms through permanent labeling of transiently activated cells. Overall, this project will enable to push back the frontiers of biological imaging providing innovative tools to interrogate quantitatively and comprehensively living systems at the molecular, cellular and network levels.

Publications

List of publications.
year	authors and title	journal	last update
2019	Tiphaine PÃ©resse, Arnaud Gautier Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging published pages: 6142, ISSN: 1422-0067, DOI: 10.3390/ijms20246142	International Journal of Molecular Sciences 20/24	2020-01-28
2019	Alison G. Tebo, Arnaud Gautier A split fluorescent reporter with rapid and reversible complementation published pages: 2822, ISSN: 2041-1723, DOI: 10.1038/s41467-019-10855-0	Nature Communications 10/1	2020-01-28
2018	Alison G. Tebo, Frederico M. Pimenta, Martha Zoumpoulaki, Carlos Kikuti, Helena Sirkia, Marie-Aude Plamont, Anne Houdusse, Arnaud Gautier Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors published pages: 2392-2397, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b00417	ACS Chemical Biology 13/9	2020-01-16
2018	Arnaud Gautier, Alison G. Tebo Fluorogenic Protein-Based Strategies for Detection, Actuation, and Sensing published pages: 1800118, ISSN: 0265-9247, DOI: 10.1002/bies.201800118	BioEssays 40/10	2020-01-16
2018	Alison G. Tebo, Frederico M. Pimenta, Yu Zhang, Arnaud Gautier Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy published pages: 5648-5653, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00649	Biochemistry 57/39	2020-01-16

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The information about "FLUOSWITCH" are provided by the European Opendata Portal: CORDIS opendata.