FLUOSWITCH SIGNED
Pushing the frontiers of biological imaging with genetically encoded fluorescence switches
Total Cost €
0EC-Contrib. €
0Partnership
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Explore the words cloud of the FLUOSWITCH project. It provides you a very rough idea of what is the project "FLUOSWITCH" about.
Project "FLUOSWITCH" data sheet
The following table provides information about the project.
| Coordinator |
SORBONNE UNIVERSITE
Organization address contact info |
| Coordinator Country | France [FR] |
| Total cost | 2˙000˙000 € |
| EC max contribution | 2˙000˙000 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2016-COG |
| Funding Scheme | ERC-COG |
| Starting year | 2017 |
| Duration (year-month-day) | from 2017-05-01 to 2022-04-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | SORBONNE UNIVERSITE | FR (PARIS) | coordinator | 851˙562.00 |
| 2 | ECOLE NORMALE SUPERIEURE | FR (PARIS CEDEX 05) | participant | 1˙148˙437.00 |
Map
Project objective
Biological imaging is essential for revealing the inner workings of living systems. Among the numerous imaging modalities, light microscopy has revolutionized biological research. In addition to advances in optics and detectors, imaging has benefited from the development of molecular tools to observe biomolecules in action. Although the last decade’s breakthroughs in imaging have led to new discoveries in biology, there are still extraordinary opportunities for basic and clinical research in further advancing imaging capabilities. This project proposes to develop new classes of probes to advance biological imaging and allow the study of biological processes in all their complexity. First, I propose to push the boundaries of multiplexing and super-resolution imaging in living cells developing a new class of fluorogenic probes that act as genetically encoded fluorescence on/off switches. Highly multiplexed images will be built up over sequential activation of orthogonal fluorescence on/off switches, while continuous switching will allow implementing innovative dynamic super-resolution techniques in living cells. Then, I will develop dynamic fluorescence on/off switches enabling to reveal the dynamics of intracellular processes, focusing in particular on the visualization of interaction dynamics in real-time, and the dynamic detection of endogenous molecules (e.g. proteins, nucleic acids) in living cells. The final part will be dedicated to the development of probes acting as molecular integrator switches to identify active cell circuits in whole tissues or organisms through permanent labeling of transiently activated cells. Overall, this project will enable to push back the frontiers of biological imaging providing innovative tools to interrogate quantitatively and comprehensively living systems at the molecular, cellular and network levels.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2019 |
Tiphaine Péresse, Arnaud Gautier Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging published pages: 6142, ISSN: 1422-0067, DOI: 10.3390/ijms20246142 |
International Journal of Molecular Sciences 20/24 | 2020-01-28 |
| 2019 |
Alison G. Tebo, Arnaud Gautier A split fluorescent reporter with rapid and reversible complementation published pages: 2822, ISSN: 2041-1723, DOI: 10.1038/s41467-019-10855-0 |
Nature Communications 10/1 | 2020-01-28 |
| 2018 |
Alison G. Tebo, Frederico M. Pimenta, Martha Zoumpoulaki, Carlos Kikuti, Helena Sirkia, Marie-Aude Plamont, Anne Houdusse, Arnaud Gautier Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors published pages: 2392-2397, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b00417 |
ACS Chemical Biology 13/9 | 2020-01-16 |
| 2018 |
Arnaud Gautier, Alison G. Tebo Fluorogenic Protein-Based Strategies for Detection, Actuation, and Sensing published pages: 1800118, ISSN: 0265-9247, DOI: 10.1002/bies.201800118 |
BioEssays 40/10 | 2020-01-16 |
| 2018 |
Alison G. Tebo, Frederico M. Pimenta, Yu Zhang, Arnaud Gautier Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy published pages: 5648-5653, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00649 |
Biochemistry 57/39 | 2020-01-16 |
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