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Pushing the frontiers of biological imaging with genetically encoded fluorescence switches

Total Cost €


EC-Contrib. €






 FLUOSWITCH project word cloud

Explore the words cloud of the FLUOSWITCH project. It provides you a very rough idea of what is the project "FLUOSWITCH" about.

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Project "FLUOSWITCH" data sheet

The following table provides information about the project.


Organization address
city: PARIS
postcode: 75006
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2022-04-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    SORBONNE UNIVERSITE FR (PARIS) coordinator 851˙562.00
2    ECOLE NORMALE SUPERIEURE FR (PARIS CEDEX 05) participant 1˙148˙437.00


 Project objective

Biological imaging is essential for revealing the inner workings of living systems. Among the numerous imaging modalities, light microscopy has revolutionized biological research. In addition to advances in optics and detectors, imaging has benefited from the development of molecular tools to observe biomolecules in action. Although the last decade’s breakthroughs in imaging have led to new discoveries in biology, there are still extraordinary opportunities for basic and clinical research in further advancing imaging capabilities. This project proposes to develop new classes of probes to advance biological imaging and allow the study of biological processes in all their complexity. First, I propose to push the boundaries of multiplexing and super-resolution imaging in living cells developing a new class of fluorogenic probes that act as genetically encoded fluorescence on/off switches. Highly multiplexed images will be built up over sequential activation of orthogonal fluorescence on/off switches, while continuous switching will allow implementing innovative dynamic super-resolution techniques in living cells. Then, I will develop dynamic fluorescence on/off switches enabling to reveal the dynamics of intracellular processes, focusing in particular on the visualization of interaction dynamics in real-time, and the dynamic detection of endogenous molecules (e.g. proteins, nucleic acids) in living cells. The final part will be dedicated to the development of probes acting as molecular integrator switches to identify active cell circuits in whole tissues or organisms through permanent labeling of transiently activated cells. Overall, this project will enable to push back the frontiers of biological imaging providing innovative tools to interrogate quantitatively and comprehensively living systems at the molecular, cellular and network levels.


year authors and title journal last update
List of publications.
2019 Tiphaine Péresse, Arnaud Gautier
Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
published pages: 6142, ISSN: 1422-0067, DOI: 10.3390/ijms20246142
International Journal of Molecular Sciences 20/24 2020-01-28
2019 Alison G. Tebo, Arnaud Gautier
A split fluorescent reporter with rapid and reversible complementation
published pages: 2822, ISSN: 2041-1723, DOI: 10.1038/s41467-019-10855-0
Nature Communications 10/1 2020-01-28
2018 Alison G. Tebo, Frederico M. Pimenta, Martha Zoumpoulaki, Carlos Kikuti, Helena Sirkia, Marie-Aude Plamont, Anne Houdusse, Arnaud Gautier
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
published pages: 2392-2397, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b00417
ACS Chemical Biology 13/9 2020-01-16
2018 Arnaud Gautier, Alison G. Tebo
Fluorogenic Protein-Based Strategies for Detection, Actuation, and Sensing
published pages: 1800118, ISSN: 0265-9247, DOI: 10.1002/bies.201800118
BioEssays 40/10 2020-01-16
2018 Alison G. Tebo, Frederico M. Pimenta, Yu Zhang, Arnaud Gautier
Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy
published pages: 5648-5653, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00649
Biochemistry 57/39 2020-01-16

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