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Developing the iTOP kit: Empowering scientists with efficient intracellular delivery of biologicals in hard-to-manipulate cell types

Total Cost €


EC-Contrib. €






 ENTRANCE project word cloud

Explore the words cloud of the ENTRANCE project. It provides you a very rough idea of what is the project "ENTRANCE" about.

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Project "ENTRANCE" data sheet

The following table provides information about the project.


Organization address
address: UPPSALALAAN 8
postcode: 3584 CT
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website
 Total cost 1˙514˙200 €
 EC max contribution 1˙514˙200 € (100%)
 Programme 1. H2020-EU.3.1.3. (Treating and managing disease)
 Code Call H2020-SMEINST-2-2016-2017
 Funding Scheme SME-2
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2019-10-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    NTRANS TECHNOLOGIES BV NL (UTRECHT) coordinator 1˙514˙200.00


 Project objective

NTrans Technologies BV has developed iTOP; a novel and extremely effective method to deliver highly relevant bioactive molecules (including DNA, RNA, active proteins, and their combinations) into mammalian cells. This includes the efficient delivery of such molecules inside the most biologically and biomedically relevant cell types (e.g. human primary and stem cells), which are notoriously difficult to manipulate. iTOP technology has been scientifically validated (published in the top scientific journal Cell in 2015) and patented (WO2015028969).

iTOP addresses the unmet need of researchers and drug developers to be able to transfect a range of biomolecules into biologically relevant cell models; in particular to study genetic diseases and to underpin results obtained with standard immortalized cell lines.

The ENTRANCE project includes the necessary steps towards market entry of the iTOP research-use-only (RUO) kit: The development of (i) an iTOP RUO kit and (ii) an iTOP on-line community as innovative marketing tool that will support proper use and stimulate efficient application of the technology. The ENTRANCE project will thus provide the scientific community access to the novel iTOP tool, facilitate direct access of iTOP kit users to the NTrans support team, and enable sharing of user experiences via an iTOP interactive forum.

ENTRANCE will instigate a revolution in biomedical sciences by empowering both researchers and drug developers to manipulate the most relevant cell types with unprecedented efficiency. This will allow the field to move forward at a significantly accelerated pace.

The iTOP RUO kit will generously earn back the investments within 4 years, and help to create a buzz around the merits and possibilities of the technology. The latter is of immense value to the SME forecasted to support 16 FTE by 2020, with the ambition to expand its product pipeline and become leader in technologies for delivery of therapeutic biomolecules into primary cells.


List of deliverables.
3.4b iTOP community platform completed Websites, patent fillings, videos etc. 2020-03-24 11:33:03
1.5b First lot of sample kit produced Demonstrators, pilots, prototypes 2020-02-27 15:22:05
1.3 Digital user manuals completed Documents, reports 2020-02-27 15:21:52
3.5b Final version of the DMP Documents, reports 2019-07-30 13:59:42
3.1b Report of the Mid-Term event Documents, reports 2019-05-31 11:55:55
3.3a Dedicated web pages for ENTRANCE Websites, patent fillings, videos etc. 2019-05-31 11:55:51
3.1a Report of the Kick-off Event Documents, reports 2019-05-31 11:55:52
3.5a First version of DMP Documents, reports 2019-05-31 11:56:04

Take a look to the deliverables list in detail:  detailed list of ENTRANCE deliverables.

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The information about "ENTRANCE" are provided by the European Opendata Portal: CORDIS opendata.

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