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Sialoglycan Array SIGNED

Generation of a Cell-Based Sialoglycan Array to Decipher Biological Interactions and Functions of the Human Sialome

Total Cost €

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EC-Contrib. €

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Partnership

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Project "Sialoglycan Array" data sheet

The following table provides information about the project.

Coordinator
KOBENHAVNS UNIVERSITET 

Organization address
address: NORREGADE 10
city: KOBENHAVN
postcode: 1165
website: www.ku.dk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Total cost 200˙194 €
 EC max contribution 200˙194 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KOBENHAVNS UNIVERSITET DK (KOBENHAVN) coordinator 200˙194.00

Map

 Project objective

Human cells display a staggering repertoire of different sialic acid-carrying glycans (sialoglycans) at the surface - the Sialome. The Sialome regulates numerous biological processes including cell communication and migration and is involved in diseases such as infections , autoimmunity and cancer. However, most biological interactions and functions of the Sialome remain elusive, because suitable methods to address the Sialome as an ‘Ome’ and to dissect specific sialoglycan functions in the cell context are lacking. Therefore, I propose to generate a cell-based sialoglycan array for the display of all human sialoglycans in the natural context of individual glycoconjugates at the cell surface in an arrayable format. Using precision genome editing (CRISPR/Cas9) genes involved in sialoglycan synthesis will be targeted to create a library of isogenic human cells each displaying a unique part of the Sialome. These isogenic cells together form the cell-based sialoglycan array and will be used for high-throughput screening and discovery of binding specificities of sialoglycan-binding proteins such as Siglecs, selectins and factor H. Finally, the cell-based sialoglycan array will be used for the production of a representative panel of recombinant glycoproteins carrying homogenous N-linked or O-linked sialoglycans. The proposed research will take place at the at the Copenhagen Center for Glycomics a world leader in Glycomics and pioneer in precision genome editing in Glycobiology where I will be trained in precision genome editing, glycoengineering and state-of-the-art glycan mass spectrometry. Altogether, the cell-based sialoglycan array will be indefinitely sustainable, addressable by any multiplex assay, and serve as production platform for recombinant sialoglycoproteins. Ultimately, I expect that the proposed cell-based sialoglycan array will open the door for wide discovery of biological interactions and functions and of the human Sialome.

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The information about "SIALOGLYCAN ARRAY" are provided by the European Opendata Portal: CORDIS opendata.

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