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OPTOLEADER SIGNED

Optogenetic control of leader cell mechanobiology during collective cell migration

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 OPTOLEADER project word cloud

Explore the words cloud of the OPTOLEADER project. It provides you a very rough idea of what is the project "OPTOLEADER" about.

epithelial    flocks    physical    modulate    emergent    behaviours    intracellular    vivo    prominently    proteins    physically    expressing    blue    molecular    express    rhogtpases    quantification    waves    influence    integral    reversible    play    collective    active    physiologically    situations    traction    groups    confluent    monolayer    physics    substrates    transduction    dimensional    leader    actions    motility    cell    activators    largely    modifications    forces    ecm    perform    latter    guide    cancer    optogenetics    exhibiting    healing    orchestrated    migratory    array    mechanical    confined    matrix    migrating    arising    paving    microscopy    transduced    create    invasion    wound    performed    migration    adhesion    phenomena    cells    tools    multicellular    force    experimental    cohesive    hallmark    extracellular    directional    stress    combines    light    leaders    fundamental    functionalized    contribution    shed    morphogenesis    monolayers    biology    generate    sensitive    biological    flocking   

Project "OPTOLEADER" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA 

Organization address
address: CARRER BALDIRI REIXAC PLANTA 2A 10-12
city: BARCELONA
postcode: 8028
website: http://www.ibecbarcelona.eu

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 158˙121 €
 EC max contribution 158˙121 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA ES (BARCELONA) coordinator 158˙121.00

Map

 Project objective

Emergent collective behaviours such as flocks and waves are a hallmark of biological active matter, and occur prominently also in large groups of migrating epithelial cells, where they are involved in fundamental biological processes such as wound healing, morphogenesis and cancer cell invasion. Physical forces transduced between cells and arising between cells and the extracellular matrix (ECM) play an integral role in orchestrated multicellular phenomena. Another integral contribution is given by the actions of leader cells, that modulate and guide the migration of cohesive cell groups. How leader cells achieve this using intracellular and cell-ECM forces remains largely to be understood. Here we propose an experimental approach to generate leader cells using optogenetics and to study how leaders influence the collective behaviour of migratory cell groups. We will use epithelial cells expressing light-sensitive activators of RhoGTPases, which enable reversible and directional control of cell motility using blue light. Traction force microscopy and monolayer stress microscopy will be performed using these cells while we will create and control leaders. With functionalized substrates, we will study the mechanical role of leaders in different conditions, from confined two-cell systems to confluent monolayers exhibiting flocking. Finally, we will express the light-sensitive proteins in cancer cells, to study how their three-dimensional dissemination is affected by leaders. Our experimental approach combines physics-derived modelling and quantification of forces with advanced molecular biology tools. The latter will enable us to perform selected modifications on cells, targeting a wide array of proteins involved in cell-cell adhesion and force transduction. Our goal is to shed light on how leaders physically influence collective migration in physiologically relevant situations, paving the way for the in vivo applications of light-induced leader cells.

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The information about "OPTOLEADER" are provided by the European Opendata Portal: CORDIS opendata.

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