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EvoTrap SIGNED

Mechanisms to emerge and replicate the first sequence information of life in geothermal microfluidics of early Earth

Total Cost €

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EC-Contrib. €

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Partnership

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 EvoTrap project word cloud

Explore the words cloud of the EvoTrap project. It provides you a very rough idea of what is the project "EvoTrap" about.

microfluidic    flows    chemistry    accumulated    onset    lc    small    pores    tof    adp    selective    illumina    ribozymes    equilibrium    prebiotic    thermophoresis    autonomous    can    taq    limited    boundary    drive    forms    modern    explore    accumulate    gelation    starting    polymerization    rna    mechanisms    primitive    convection    60    life    bind    respective    richert    ligase    molecules    water    3d    select    trigger    dna    food    microfluidics    lt    host    200mbar    atp    pressures    enhanced    interfaces    sequences    deg    progressing    edc    boiling    interesting    hypercycle    first    strands    evolution    fold    hydro    settings    mimicking    heated    nbsp    separated    szostak    gradient    creation    heat    chemistries    pressure    joyce    lab    cooperative    sequence    base    air    metabolism    ligation    ms    dynamics    800    thermal    biochemistry    monitor    temperature    earth    fast    replication    expand    explored    activated    reconstruct    recondensation    below    oligonucleotides    fog    rock    capillary    oligonucleotide    mimick    expertise    emergence    boost    molecular    sequencing    gradients    printed    tested   

Project "EvoTrap" data sheet

The following table provides information about the project.

Coordinator
LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN 

Organization address
address: GESCHWISTER SCHOLL PLATZ 1
city: MUENCHEN
postcode: 80539
website: www.uni-muenchen.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙364˙500 €
 EC max contribution 2˙364˙500 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-10-01   to  2023-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN DE (MUENCHEN) coordinator 2˙364˙500.00

Map

 Project objective

Can we reconstruct in the lab the onset of molecular evolution? To trigger the autonomous emergence of the first oligonucleotide sequences, we will explore non-equilibrium boundary conditions and selective mechanisms to host the fast progressing prebiotic replication chemistry of oligonucleotides. We will explore novel water-fog microfluidic settings to boost the replication and selection of the first RNA sequences. The findings aims to enable the creation of primitive life forms in the lab, starting from simple molecules in heated rock pores of early Earth.

Autonomous replication and metabolism. We will expand our thermal gradient expertise to host three replication chemistries. Using 3D printed microfluidics, we will mimick conditions in pores on early Earth. Thermophoresis will select long over short strands, accumulate small food molecules and strands will be separated by thermal convection and novel mechanisms in water-air systems. With respective collaboration partners, we will drive the replication from RNA ribozymes (Joyce), base-by-base RNA replication (Szostak) and EDC activated DNA ligation (Richert) and monitor the results with Illumina sequencing and TOF LC/MS. The ligation will be also explored with Taq ligase since we expect a cooperative replication dynamics with hypercycle-like characteristics. Thermal gradients will drive early metabolism to boost RNA polymerization and select ATP over ADP to drive modern biochemistry.

Sequence selection in low pressure water-air systems. Oligonucleotides bind to water-air interfaces. and can be accumulated 800-fold by heat-driven capillary flows. Based on this, we expect interesting selection effects under microfluidic boiling, fog formation and recondensation dynamics. The settings are tested for sequence selective hydro-gelation of RNA/DNA and enhanced replication chemistry. The temperature of boiling water will be limited below 60°C by using air pressures <200mbar, mimicking very early Earth conditions.

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The information about "EVOTRAP" are provided by the European Opendata Portal: CORDIS opendata.

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