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EvoTrap SIGNED

Mechanisms to emerge and replicate the first sequence information of life in geothermal microfluidics of early Earth

Total Cost €

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EC-Contrib. €

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Partnership

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 EvoTrap project word cloud

Explore the words cloud of the EvoTrap project. It provides you a very rough idea of what is the project "EvoTrap" about.

joyce    richert    microfluidics    biochemistry    szostak    metabolism    gradient    forms    select    progressing    onset    ligation    temperature    edc    deg    convection    dna    mechanisms    rock    reconstruct    starting    gelation    primitive    capillary    molecules    60    lc    hydro    expertise    oligonucleotides    water    hypercycle    settings    prebiotic    microfluidic    fog    fold    800    respective    3d    200mbar    fast    adp    expand    drive    pressure    lab    illumina    polymerization    boundary    mimicking    selective    sequencing    separated    small    emergence    equilibrium    trigger    rna    accumulate    thermophoresis    interfaces    ribozymes    sequence    thermal    limited    gradients    autonomous    earth    taq    pressures    chemistry    enhanced    mimick    explore    chemistries    boiling    life    sequences    activated    can    tested    bind    interesting    heated    first    recondensation    ligase    boost    tof    nbsp    air    base    replication    atp    flows    food    ms    dynamics    creation    monitor    strands    oligonucleotide    below    heat    pores    cooperative    modern    accumulated    printed    host    lt    evolution    explored    molecular   

Project "EvoTrap" data sheet

The following table provides information about the project.

Coordinator
LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN 

Organization address
address: GESCHWISTER SCHOLL PLATZ 1
city: MUENCHEN
postcode: 80539
website: www.uni-muenchen.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙364˙500 €
 EC max contribution 2˙364˙500 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-10-01   to  2023-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN DE (MUENCHEN) coordinator 2˙364˙500.00

Map

 Project objective

Can we reconstruct in the lab the onset of molecular evolution? To trigger the autonomous emergence of the first oligonucleotide sequences, we will explore non-equilibrium boundary conditions and selective mechanisms to host the fast progressing prebiotic replication chemistry of oligonucleotides. We will explore novel water-fog microfluidic settings to boost the replication and selection of the first RNA sequences. The findings aims to enable the creation of primitive life forms in the lab, starting from simple molecules in heated rock pores of early Earth.

Autonomous replication and metabolism. We will expand our thermal gradient expertise to host three replication chemistries. Using 3D printed microfluidics, we will mimick conditions in pores on early Earth. Thermophoresis will select long over short strands, accumulate small food molecules and strands will be separated by thermal convection and novel mechanisms in water-air systems. With respective collaboration partners, we will drive the replication from RNA ribozymes (Joyce), base-by-base RNA replication (Szostak) and EDC activated DNA ligation (Richert) and monitor the results with Illumina sequencing and TOF LC/MS. The ligation will be also explored with Taq ligase since we expect a cooperative replication dynamics with hypercycle-like characteristics. Thermal gradients will drive early metabolism to boost RNA polymerization and select ATP over ADP to drive modern biochemistry.

Sequence selection in low pressure water-air systems. Oligonucleotides bind to water-air interfaces. and can be accumulated 800-fold by heat-driven capillary flows. Based on this, we expect interesting selection effects under microfluidic boiling, fog formation and recondensation dynamics. The settings are tested for sequence selective hydro-gelation of RNA/DNA and enhanced replication chemistry. The temperature of boiling water will be limited below 60°C by using air pressures <200mbar, mimicking very early Earth conditions.

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The information about "EVOTRAP" are provided by the European Opendata Portal: CORDIS opendata.

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