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HighThroughFROGS SIGNED

Reviving ghosts of taxonomy past: Identifying cryptic species using high-throughput sequencing of historical museum specimens for Asian ranids with gastromyzophorous tadpoles

Total Cost €

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EC-Contrib. €

0

Partnership

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 HighThroughFROGS project word cloud

Explore the words cloud of the HighThroughFROGS project. It provides you a very rough idea of what is the project "HighThroughFROGS" about.

quantifying    conservation    data    diversity    defied    sklodowska    tissue    advancing    usa    tropical    museums    mtdna    focal    date    marie    obtain    ranid    decades    aligned    sequencing    housed    myself    raw    phylogenomic    collaborations    gastromyzophory    exceptional    historical    independent    capture    disseminate    molecular    first    tested    specimens    systematics    evolution    valuable    phylogenetic    taxonomic    actions    gastromyzophorous    clarification    tadpoles    modern    protocols    frogs    countries    exome    catalyst    cryptic    techniques    horizon    career    obtaining    apart    materials    sequence    genomic    my    germany    postdoctoral    retrieving    position    actively    hts    play    genome    speciation    species    2020    freshly    taxa    researcher    tropics    curie    ago    degraded    history    asian    biogeographic    audience    dna    fragment    se    sanger    phylogenetics    genetic    emphasize    indonesia    collected    biodiversity    feasibility    baseline    natural    museum    science    designed    tools    critical    samples    group   

Project "HighThroughFROGS" data sheet

The following table provides information about the project.

Coordinator		 UNIVERSITAET HAMBURG 

Organization address
address: MITTELWEG 177
city: HAMBURG
postcode: 20148
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Germany [DE]
 Total cost 246˙669 €
 EC max contribution 246˙669 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-GF
 Starting year 2020
 Duration (year-month-day) from 2020-03-01   to  2023-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET HAMBURG DE (HAMBURG) coordinator 246˙669.00
2    THE REGENTS OF THE UNIVERSITY OF CALIFORNIA US (OAKLAND CA) partner 0.00

Map

 Project objective

My postdoctoral study is designed to develop protocols and procedures to obtain genomic DNA data from degraded old museum specimens using HTS (e.g., exome-capture and/ or mtDNA genome sequencing) using SE Asian frogs as focal taxa. Specimens housed in natural history museums are essential raw materials for studies of cryptic speciation that is very common in the tropics. These studies require molecular genetic tools that, to date, could only be applied to freshly collected tissue samples. Because HTS is based on short-fragment sequencing technology, it is more effective at obtaining sequence data from historical specimens than Sanger sequencing, particularly for very old samples collected more than 100 years ago. HTS methods will be tested to assess the phylogenetic and taxonomic position of the Asian ranid frogs with gastromyzophorous tadpoles, a group that has defied taxonomic clarification for decades. This proposed study is well aligned to the Work Programme of Horizon 2020 Marie Sklodowska-Curie Actions. The results of this study will be a valuable baseline to assess the feasibility of and to improve exome-capture techniques aimed at retrieving historical DNA from type specimens for future studies. This study will be the first to investigate the evolution of gastromyzophory in Asian ranid frogs. It will also emphasize the need to apply modern techniques in tropical countries with high biodiversity as a critical step in quantifying its diversity and developing conservation actions for cryptic species. Apart from that, this study is an exceptional step towards advancing my career and establish myself as an independent researcher in the field of phylogenetics systematics. Furthermore, I will be able to actively contribute to disseminate my science to broader audience and play an important role as a catalyst for a long term collaborations between the institutions in Germany, USA, and Indonesia in phylogenomic, systematics, and biogeographic studies.

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The information about "HIGHTHROUGHFROGS" are provided by the European Opendata Portal: CORDIS opendata.

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