Opendata, web and dolomites

APTAFRAME SIGNED

DNA-origami frame platform for co-evolution ligand selection

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 APTAFRAME project word cloud

Explore the words cloud of the APTAFRAME project. It provides you a very rough idea of what is the project "APTAFRAME" about.

generation    antibodies    economaffinity    systematically    tools    strategy    contrast    avidity    rational    multidimensional    predefined    systematic    diseases    structural    biotechnology    combination    form    platform    diagnostics    accessible    transformational    cryo    provides    smaller    therapy    power    fv    complexes    leveraging    ligands    framework    economic    aptamers    microscopy    generate    yielding    em    coverage    interrogation    expedites    interactions    electron    structures    chain    cover    co    paramount    structure    affinity    nanotechnology    once    recognition    reconstructions    addressability    dimensional    vitro    reagents    reconstruction    origami    ligand    feedback    despite    proteome    parallel    scfv    fragments    spatial    molecular    dna    conjunction    cooperative    resolved    single    biomolecules    scientific    discovery    100kda    antibody    biosensor    exploits    tool    direct    lt    context    toolbox    binding    evolution    epitopes    treatment    libraries   

Project "APTAFRAME" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

Organization address
address: POLARIS HOUSE NORTH STAR AVENUE
city: SWINDON
postcode: SN2 1FL
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2019
 Duration (year-month-day) from 2019-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 212˙933.00

Map

 Project objective

Affinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economAffinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economic impact it remains challenging to systematically generate high-affinity ligands to cover all epitopes of a given target. Here I propose a strategy to provide spatial control over ligand discovery process leveraging the power of in vitro evolution in conjunction with the spatial addressability of DNA nanotechnology methods. Together they form a new discovery platform for the systematic and parallel generation of ligands, specifically aptamers and single-chain Fv antibody fragments (scFv), to provide coverage of epitopes at predefined targets. The proposed strategy exploits cooperative binding (avidity), by co-evolution of the affinity reagents, in the context of a defined molecular three-dimensional framework provided by DNA origami structures. This further expedites structure determination of ligand-target complexes by cryo-electron microscopy (cryo-EM). The combination of rational framework design, in vitro evolution and structural feedback provided by cryo-EM reconstructions will provide a toolbox for the systematic generation of ligands to all accessible epitopes. Our approach also provides a tool for electron microscopy reconstruction of smaller (< 100kDa) biomolecules and their molecular interactions by enhancing contrast and providing context. Once established, this approach will provide a transformational technology platform that enables the parallel interrogation of multidimensional, spatially resolved libraries, yielding cooperative ligands for highly specific target recognition, with direct applications in biosensor development, proteome analysis, diagnostics and therapy.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "APTAFRAME" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "APTAFRAME" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

EVOMET (2019)

The rise and fall of metastatic clones under immune attack

Read More  

TOPOCIRCUS (2019)

Simulations of Topological Phases in Superconducting Circuits

Read More  

ReSOLeS (2019)

New Reconfigurable Spectrum Optical Fibre Laser Sources

Read More