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APTAFRAME SIGNED

DNA-origami frame platform for co-evolution ligand selection

Total Cost €

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EC-Contrib. €

0

Partnership

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 APTAFRAME project word cloud

Explore the words cloud of the APTAFRAME project. It provides you a very rough idea of what is the project "APTAFRAME" about.

microscopy    predefined    antibody    reconstructions    treatment    tools    resolved    despite    combination    power    scientific    binding    cover    context    nanotechnology    avidity    lt    conjunction    cooperative    structural    electron    reagents    generate    transformational    provides    antibodies    generation    origami    spatial    interrogation    fragments    interactions    epitopes    dimensional    economaffinity    feedback    ligand    therapy    vitro    addressability    100kda    platform    scfv    em    fv    accessible    reconstruction    proteome    single    leveraging    biosensor    systematic    ligands    chain    biotechnology    parallel    cryo    structure    direct    structures    recognition    complexes    dna    framework    rational    once    co    toolbox    strategy    expedites    diagnostics    yielding    economic    form    discovery    smaller    contrast    systematically    libraries    tool    biomolecules    affinity    molecular    coverage    diseases    multidimensional    evolution    paramount    aptamers    exploits   

Project "APTAFRAME" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

Organization address
address: POLARIS HOUSE NORTH STAR AVENUE
city: SWINDON
postcode: SN2 1FL
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2019
 Duration (year-month-day) from 2019-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 212˙933.00

Map

 Project objective

Affinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economAffinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economic impact it remains challenging to systematically generate high-affinity ligands to cover all epitopes of a given target. Here I propose a strategy to provide spatial control over ligand discovery process leveraging the power of in vitro evolution in conjunction with the spatial addressability of DNA nanotechnology methods. Together they form a new discovery platform for the systematic and parallel generation of ligands, specifically aptamers and single-chain Fv antibody fragments (scFv), to provide coverage of epitopes at predefined targets. The proposed strategy exploits cooperative binding (avidity), by co-evolution of the affinity reagents, in the context of a defined molecular three-dimensional framework provided by DNA origami structures. This further expedites structure determination of ligand-target complexes by cryo-electron microscopy (cryo-EM). The combination of rational framework design, in vitro evolution and structural feedback provided by cryo-EM reconstructions will provide a toolbox for the systematic generation of ligands to all accessible epitopes. Our approach also provides a tool for electron microscopy reconstruction of smaller (< 100kDa) biomolecules and their molecular interactions by enhancing contrast and providing context. Once established, this approach will provide a transformational technology platform that enables the parallel interrogation of multidimensional, spatially resolved libraries, yielding cooperative ligands for highly specific target recognition, with direct applications in biosensor development, proteome analysis, diagnostics and therapy.

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The information about "APTAFRAME" are provided by the European Opendata Portal: CORDIS opendata.

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