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APTAFRAME SIGNED

DNA-origami frame platform for co-evolution ligand selection

Total Cost €

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EC-Contrib. €

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Partnership

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 APTAFRAME project word cloud

Explore the words cloud of the APTAFRAME project. It provides you a very rough idea of what is the project "APTAFRAME" about.

feedback    parallel    epitopes    context    systematically    vitro    despite    smaller    diagnostics    structural    rational    economic    interrogation    multidimensional    electron    generate    microscopy    reconstructions    single    molecular    cover    avidity    interactions    leveraging    accessible    scientific    contrast    tool    affinity    discovery    strategy    aptamers    ligand    origami    economaffinity    systematic    therapy    paramount    100kda    expedites    complexes    spatial    form    recognition    fragments    cryo    structures    addressability    evolution    binding    transformational    generation    dimensional    dna    exploits    proteome    co    reagents    structure    em    conjunction    direct    coverage    biotechnology    resolved    antibodies    fv    yielding    reconstruction    provides    combination    platform    ligands    treatment    antibody    biomolecules    tools    diseases    biosensor    libraries    toolbox    scfv    cooperative    chain    power    once    framework    predefined    nanotechnology    lt   

Project "APTAFRAME" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

Organization address
address: POLARIS HOUSE NORTH STAR AVENUE
city: SWINDON
postcode: SN2 1FL
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2019
 Duration (year-month-day) from 2019-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 212˙933.00

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 Project objective

Affinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economAffinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economic impact it remains challenging to systematically generate high-affinity ligands to cover all epitopes of a given target. Here I propose a strategy to provide spatial control over ligand discovery process leveraging the power of in vitro evolution in conjunction with the spatial addressability of DNA nanotechnology methods. Together they form a new discovery platform for the systematic and parallel generation of ligands, specifically aptamers and single-chain Fv antibody fragments (scFv), to provide coverage of epitopes at predefined targets. The proposed strategy exploits cooperative binding (avidity), by co-evolution of the affinity reagents, in the context of a defined molecular three-dimensional framework provided by DNA origami structures. This further expedites structure determination of ligand-target complexes by cryo-electron microscopy (cryo-EM). The combination of rational framework design, in vitro evolution and structural feedback provided by cryo-EM reconstructions will provide a toolbox for the systematic generation of ligands to all accessible epitopes. Our approach also provides a tool for electron microscopy reconstruction of smaller (< 100kDa) biomolecules and their molecular interactions by enhancing contrast and providing context. Once established, this approach will provide a transformational technology platform that enables the parallel interrogation of multidimensional, spatially resolved libraries, yielding cooperative ligands for highly specific target recognition, with direct applications in biosensor development, proteome analysis, diagnostics and therapy.

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The information about "APTAFRAME" are provided by the European Opendata Portal: CORDIS opendata.

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