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APTAFRAME SIGNED

DNA-origami frame platform for co-evolution ligand selection

Total Cost €

0

EC-Contrib. €

0

Partnership

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 APTAFRAME project word cloud

Explore the words cloud of the APTAFRAME project. It provides you a very rough idea of what is the project "APTAFRAME" about.

treatment    generate    fv    ligands    provides    economaffinity    electron    power    reconstruction    interrogation    cryo    fragments    proteome    microscopy    cover    predefined    em    platform    discovery    structural    aptamers    interactions    yielding    vitro    form    expedites    transformational    exploits    diseases    paramount    affinity    100kda    epitopes    ligand    coverage    accessible    resolved    lt    reconstructions    spatial    structures    toolbox    addressability    strategy    framework    co    complexes    single    recognition    smaller    rational    nanotechnology    scientific    despite    systematic    biomolecules    tools    generation    binding    cooperative    multidimensional    context    conjunction    biosensor    structure    scfv    economic    direct    dna    systematically    tool    origami    feedback    biotechnology    dimensional    parallel    leveraging    avidity    contrast    reagents    evolution    diagnostics    antibody    antibodies    molecular    once    combination    chain    libraries    therapy   

Project "APTAFRAME" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

Organization address
address: POLARIS HOUSE NORTH STAR AVENUE
city: SWINDON
postcode: SN2 1FL
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2019
 Duration (year-month-day) from 2019-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 212˙933.00

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 Project objective

Affinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economAffinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economic impact it remains challenging to systematically generate high-affinity ligands to cover all epitopes of a given target. Here I propose a strategy to provide spatial control over ligand discovery process leveraging the power of in vitro evolution in conjunction with the spatial addressability of DNA nanotechnology methods. Together they form a new discovery platform for the systematic and parallel generation of ligands, specifically aptamers and single-chain Fv antibody fragments (scFv), to provide coverage of epitopes at predefined targets. The proposed strategy exploits cooperative binding (avidity), by co-evolution of the affinity reagents, in the context of a defined molecular three-dimensional framework provided by DNA origami structures. This further expedites structure determination of ligand-target complexes by cryo-electron microscopy (cryo-EM). The combination of rational framework design, in vitro evolution and structural feedback provided by cryo-EM reconstructions will provide a toolbox for the systematic generation of ligands to all accessible epitopes. Our approach also provides a tool for electron microscopy reconstruction of smaller (< 100kDa) biomolecules and their molecular interactions by enhancing contrast and providing context. Once established, this approach will provide a transformational technology platform that enables the parallel interrogation of multidimensional, spatially resolved libraries, yielding cooperative ligands for highly specific target recognition, with direct applications in biosensor development, proteome analysis, diagnostics and therapy.

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The information about "APTAFRAME" are provided by the European Opendata Portal: CORDIS opendata.

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