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TOC-maker SIGNED

The assembly and structure of the chloroplast protein import machinery in plants

Total Cost €

0

EC-Contrib. €

0

Partnership

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 TOC-maker project word cloud

Explore the words cloud of the TOC-maker project. It provides you a very rough idea of what is the project "TOC-maker" about.

structural    envelope    tightly    gap    etiolation    composition    chase    analysing    expressing    enhanced    photosynthesis    dynamic    toc    de    proper    cryo    nature    organization    microscopy    difficult    integration    encoded    affinity    rapid    assist    tagged    plant    import    transiently    ribosomes    machinery    outer    specialized    translocons    synthesis    plants    pulse    cells    compartment    coupled    tic    equipped    polypeptides    molecular    purification    arabidopsis    elucidated    model    membranes    apparatus    chloroplast    biochemical    nascent    convert    nucleus    predicted    enriched    electron    purpose    chemical    majorly    mature    chloroplasts    solar    epitope    stalled    transgenic    interacting    protein    assembled    photosynthetic    inner    respectively    abundant    assembly    components    events    cell    reported    resolution    homeostasis    configurations    sequence    thaliana    energy    complexes    techniques    housekeeping    multiprotein    biogenesis    purified    mechanisms    experiments    proteins   

Project "TOC-maker" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 224˙933 €
 EC max contribution 224˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-06-01   to  2022-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 224˙933.00

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 Project objective

Plants convert solar energy into chemical energy by the process called photosynthesis in a specialized compartment of the cell known as chloroplasts. Chloroplasts are majorly enriched with nucleus-encoded proteins and to import them, chloroplast outer and inner envelope membranes are equipped with apparatus called the TOC and TIC translocons, respectively. For the TOC apparatus, there are two major configurations, TOC-P and TOC-H, reported so far, which import highly abundant, Photosynthetic and Housekeeping pre-proteins, respectively. TOC-P and TOC-H are multiprotein complexes which must be specifically assembled for proper development and homeostasis of the chloroplast. Due to the dynamic nature of the translocons, component synthesis and assembly must be rapid and tightly coupled, making the process difficult to investigate. Thus, understanding the mechanisms of the assembly process is both challenging and exciting. Biogenesis of TOC complexes is rapidly enhanced during chloroplast development or de-etiolation, and I will exploit this process to investigate the assembly of different TOC configurations, using the model plant Arabidopsis thaliana. For this purpose, transgenic plants expressing epitope-tagged TOC components and cells expressing nascent polypeptides of TOC components with stalled ribosomes will be generated. Proteins transiently interacting with new TOC components, which are predicted to assist integration and assembly of the TOC complex, will be studied by using affinity purification, pulse-chase experiments, and other biochemical techniques, and thus a sequence of assembly events will be elucidated. Although the molecular composition of the TOC protein import machinery has been well studied, the detailed structural organization of TOC complexes has not yet been elucidated. I will address this knowledge gap by analysing affinity-purified TOC complexes from mature chloroplasts at high resolution by cryo-electron microscopy.

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lastchecktime (2022-08-11 11:18:09) correctly updated