EVO-CHROMO

Sperm chromatin and the evolution of chromatin architecture at regulatory regions in animal genomes

Coordinatore	FUNDACION PRIVADA INSTITUT DE MEDICINA PREDICTIVA I PERSONALITZADA DEL CANCER    more  Organization address address: CAMI DE LES ESCOLES CR CAN RUTI s/n city: Badalona postcode: 8916 contact info Titolo: Mr. Nome: Oscar Cognome: Fraile Email: send email Telefono: +34 554 30 52
Nazionalità Coordinatore	Spain [ES]
Totale costo	45˙000 €
EC contributo	45˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-ERG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-01-01   -   2014-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	FUNDACION PRIVADA INSTITUT DE MEDICINA PREDICTIVA I PERSONALITZADA DEL CANCER  Organization address address: CAMI DE LES ESCOLES CR CAN RUTI s/n city: Badalona postcode: 8916 contact info Titolo: Mr. Nome: Oscar Cognome: Fraile Email: send email Telefono: +34 554 30 52	ES (Badalona)	coordinator	45˙000.00

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 Obiettivo del progetto (Objective)

'The genome of eukaryotic cells is packaged into chromatin. Recent technological advances have allowed the profiling of chromatin on a genome-wide scale in a diversity of living species. We are now faced with an excellent opportunity to study chromatin function and evolution in a way that was not possible before. Here, I propose to study the mechanisms of nucleosome retention in human sperm and the role and evolution of chromatin architecture at regulatory elements of animal genomes. In human sperm, most of the genome is packaged by protamines and not by histones. The 4% of the human genome that remains packaged in nucleosomes tends to contain gene promoters and developmental regulatory genes. However, there is no mechanism to explain why nucleosomes are retained at these locations. To this end, I will test three possible mechanisms of nucleosome retention in human sperm. This information will help us understand the mechanisms that may affect epigenetic inheritance. As a second aspect of this project, I propose to study the interplay between chromatin structure and regulatory evolution in animal genomes. Nucleosome positions have been mapped throughout five entire animal genomes; human, mouse, Japanese killifish (Oryzias latipes), Caenorhabditis elegans and Drosophila melanogaster. I propose to use this data and the intrinsic affinity for nucleosomes predicted from in vitro nucleosome reconstitution experiments to study the role of nucleosome positioning in the evolution of promoters and distal enhancers in metazoans. The grant will be used for the transition from a post-doctoral to an independent research position and will contribute to my integration in a major scientific hub in Europe.'