DENDRITIC PROCESSING

Local Processing of Dendritically Synthesized Membrane Proteins

Coordinatore	MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.    more  Organization address address: Hofgartenstrasse 8 city: MUENCHEN postcode: 80539 contact info Titolo: Dr. Nome: Antje Maria Cognome: Berken Email: send email Telefono: +48 69 5068202001 Fax: +48 69 5068202002
Nazionalità Coordinatore	Germany [DE]
Totale costo	75˙000 €
EC contributo	75˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-CIG
Funding Scheme	MC-CIG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-04-01   -   2015-03-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.  Organization address address: Hofgartenstrasse 8 city: MUENCHEN postcode: 80539 contact info Titolo: Dr. Nome: Antje Maria Cognome: Berken Email: send email Telefono: +48 69 5068202001 Fax: +48 69 5068202002	DE (MUENCHEN)	coordinator	75˙000.00

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 Obiettivo del progetto (Objective)

'The local synthesis and degradation of proteins represent a particularly efficient means to adaptively modify the composition of targeted dendritic branches and associated synapses. Recent data from our laboratory show that numerous mRNA encoding neurotransmitter receptors and voltage-gated channels are enriched in distal dendrites, indicating that local translation sustains local membrane protein turnover and may thus regulate dendritic excitability. In contrast to cytoplasmic proteins, local production of cell-surface proteins requires their processing by the secretory apparatus. Although the list of dendritic mRNAs encoding membrane and secreted proteins keeps growing, whether and how dendritically synthesized membrane proteins are locally processed by secretory organelles is still largely elusive.

Here we propose to assess the dendritic synthesis and turnover of key neurotransmitter receptors and voltage-gated channels controlling dendritic excitability by combining genetic engineering and biochemical approaches to selectively label and purify nascent proteins in specified dendritic compartments. Furthermore, by analyzing their glycosylation status and controlling the targeting of their mRNA to distal dendrites, we aim to understand how dendritically synthesized membrane proteins are processed by dendritic secretory organelles.

The turnover and glycosylation status of neuronal ion channels in vivo is largely unknown. By assessing a large cast of synaptic receptors and voltage-gated channels, this project will hopefully allow important inferences to be made about local protein synthesis, glycosylation and protein stability in brain. In the longer run, the proposed research will enable us to focus later functional studies on molecules displaying rapid turnover, and thus more likely to be acutely affected by adaptive regulations of secretory functions.'