PICENGINEERING

Basal factor engineering and synthetic nucleosomal DNA super-template construction for pre-initiation complex assembly

 Coordinatore EUROPEAN MOLECULAR BIOLOGY LABORATORY 

 Organization address address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117

contact info
Titolo: Ms.
Nome: Sonja
Cognome: Noss
Email: send email
Telefono: +49 6221 387 8771

 Nazionalità Coordinatore Germany [DE]
 Totale costo 269˙096 €
 EC contributo 269˙096 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2011-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2012
 Periodo (anno-mese-giorno) 2012-05-01   -   2014-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY

 Organization address address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117

contact info
Titolo: Ms.
Nome: Sonja
Cognome: Noss
Email: send email
Telefono: +49 6221 387 8771

DE (HEIDELBERG) coordinator 269˙096.40

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

explore    promoter    architecture    pic    initiation    template    binding    nucleosomes    nature    ray    site    tbp    transcription    tfiia    tfiid    tata    protein       tfiib    assembly    modified    contains    dna    structural    limiting    cross   

 Obiettivo del progetto (Objective)

'Initiation of RNA polymerase II (Pol II) transcription is activated by the assembly of preinitiation complex (PIC) onto gene promoters. PIC contains the largest general transcription factor (GTF), TFIID that comprises TATA box-binding protein (TBP). The binding of TBP to TATA promoter is rate-limiting step in PIC assembly and is stabilized by GTFs, TFIIA and TFIIB. TFIID also contains reader domains that recognize specific epigenetic marks in nucleosomes located upstream and downstream of transcription start site. Structural information on the architecture of nucleosomes, DNA, TFIIA, TFIIB and TFIID in this context remains elusive to date limiting our understanding of DNA architecture at promoter site and the interaction of TFIID with epigenetically modified nucleosomes in transcription initiation. The objective of this proposal is to explore this cross-talk by creating a synthetic DNA super-template for PIC assembly containing modified nucleosomes, promoter DNA, TFIIA, TFIIB and TBP. This will be attempted via an international collaborative effort, employing integrated multidisciplinary approach including protein and DNA engineering and their supramolecular assembly combined with chemical cross-linking. This template will be analyzed with and without highly purified, fully recombinant TFIID (sub)complexes through a multi-resolution structural approach with X-ray crystallography, electron microscopy and small angle X-ray scattering techniques. This methodology will be useful to explore interactions of similar complexity involving other chromatin regulatory factors, thereby extending the horizon of transcription research or general multi-protein complex research, which is at the forefront of contemporary molecular biology. The interdisciplinary nature of this project promises to contribute to the applicant’s career development by diversifying and advancing her competencies and refining her skills ultimately guiding her towards a position of independent leadership.'

Introduzione (Teaser)

Transcription is the first step in transformation of the DNA code into actual molecules. Nature has equipped DNA with a system of checks and balances to ensure that transcription is well-regulated, and scientists are elucidating relevant structures.

Altri progetti dello stesso programma (FP7-PEOPLE)

NF-RAD (2009)

NF-RAD: Near-Field Radiation Absorption and Scattering by Nanoparticles on Surfaces

Read More  

DYNAREP (2011)

Self-Replication in Dynamic Combinatorial Libraries

Read More  

TRANSPHO (2013)

Roles of non-coding RNAs in translational regulation during root developmental adaptation to phosphate starvation

Read More