MECETERMITI

Mind the Gap: Mechanism and Impact of Ceramide Trafficking at the ER-Mitochondrial Interface

Coordinatore	UNIVERSITAET OSNABRUECK    more  Organization address address: NEUER GRABEN/SCHLOSS 29 city: OSNABRUECK postcode: 49069 contact info Titolo: Ms. Nome: Renate Cognome: Sokolowski Email: send email Telefono: 495420000000 Fax: 495420000000
Nazionalità Coordinatore	Germany [DE]
Totale costo	174˙475 €
EC contributo	174˙475 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-IEF
Funding Scheme	MC-IEF
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-06-01   -   2015-07-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITAET OSNABRUECK  Organization address address: NEUER GRABEN/SCHLOSS 29 city: OSNABRUECK postcode: 49069 contact info Titolo: Ms. Nome: Renate Cognome: Sokolowski Email: send email Telefono: 495420000000 Fax: 495420000000	DE (OSNABRUECK)	coordinator	174˙475.20

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 Obiettivo del progetto (Objective)

A principal cause of failure in the treatment of cancer is that cancer cells develop resistance to chemo- and radiotherapy, leading to recurrence of the disease or even death. Sphingolipid molecules can modulate the ability of malignant cells to grow and resist anticancer regimens, with some molecules promoting tumorigenesis and others acting as tumor-suppressors. The tumor-suppressor activity of ceramides has prompted development of different formulations of synthetic ceramides in anticancer therapies, but the poor solubility of these compounds restricts their biocompatibility. Therefore, attention is increasingly focused on possibilities to manipulate cellular sphingolipid balances from within.

The recent discovery of a ceramide sensor that protects cells against ceramide-induced cell death marks an important breakthrough. Disrupting sensor function causes accumulation of ceramides in the endoplasmic reticulum (ER) and their flow into mitochondria, triggering a mitochondrial pathway of apoptosis. While these results define the transfer of ER ceramides to mitochondria as a key determinant of cell fate, the molecular principles that govern ceramide trafficking at the ER-mitochondrial interface remain to be established.

In this project, I aim to unravel the transport mechanism by which ER ceramides can reach mitochondria to initiate apoptosis. I will conduct a chemical screen employing photoactivatable and clickable ceramide analogues to identify ceramide-binding proteins operating at the ER-mitochondrial interface. As complementary approach, I will conduct a functional screen to search for proteins required for delivering ER ceramides to mitochondria. This screen is based on the principal that blocking expression of such proteins would prevent cells with a disrupted ceramide sensor from committing suicide. Finally, I will evaluate newly-identified components of the ceramide trafficking machinery as targets for modulating drug-induced apoptosis in tumors.