CYTOSKELETONCOUPLING

Active actin-microtubule crosstalk in reconstituted systems

 Coordinatore STICHTING VOOR FUNDAMENTEEL ONDERZOEK DER MATERIE - FOM 

 Organization address address: Van Vollenhovenlaan 659
city: UTRECHT
postcode: 3527 JP

contact info
Titolo: Mr.
Nome: Bart
Cognome: Van Leijen
Email: send email
Telefono: +31 20 7547100
Fax: +31 20 7547290

 Nazionalità Coordinatore Netherlands [NL]
 Totale costo 183˙469 €
 EC contributo 183˙469 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2012-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2013
 Periodo (anno-mese-giorno) 2013-05-01   -   2015-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    STICHTING VOOR FUNDAMENTEEL ONDERZOEK DER MATERIE - FOM

 Organization address address: Van Vollenhovenlaan 659
city: UTRECHT
postcode: 3527 JP

contact info
Titolo: Mr.
Nome: Bart
Cognome: Van Leijen
Email: send email
Telefono: +31 20 7547100
Fax: +31 20 7547290

NL (UTRECHT) coordinator 183˙469.80

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

cytoskeleton    tip    centrosome    cell    filaments    mt    central    organization    components    microtubule    cells    dynamics    microtubules    network    prof    me    dynamic    mutual    crosstalk    asters    actin   

 Obiettivo del progetto (Objective)

'Living cells maintain a high degree of functional and structural organization which largely depends on a highly dynamic scaffold, the cytoskeleton, with microtubules (MTs) and actin filaments as the central elements. While both components are comparably well characterized individually, their interplay and mutual regulation remains poorly understood. Precisely their cooperative functioning, however, is increasingly regarded as a central element for many cellular key processes including cell division, cell migration, and adhesion. The central aim of this proposal is to achieve a more profound and quantitative understanding of actin-MT crosstalk using a simple yet realistic reconstituted model system. Centrosome nucleated microtubule asters will be embedded into passive and active actin networks. Coupling between the two cytoskeleton components will be introduced in form of transient binding of growing MT plus ends to actin filaments mediated by tip-tracking EB3 and MACF, a highly relevant actin-MT linker. I will study the mutual influence between a surrounding actin matrix and the dynamic MT asters. Addition of myosin motors will induce actin network contractions that should strongly affect MT growth dynamics. Applying computer-based image analysis routines will allow me to quantify centrosome motion and microtubule tip dynamics. Further, I will investigate the additional effects of cell size confinement on the spatial self-organization of actin and MT asters. Finally, I aim at mimicking the actin-MT crosstalk at the leading edge of migrating cells by establishing an actively treadmilling actin network growing against the aster-based microtubules of opposite polarity. The proposed work will be carried out at the AMOLF institute, simultaneously in the groups of Prof. M. Dogterom and Prof. G. Koenderink which allows me to integrate their combined MT and actin expertise.'

Altri progetti dello stesso programma (FP7-PEOPLE)

QUANTITY IN NUMBER (2011)

"Symbolic and non-symbolic number processing, a developmental perspective"

Read More  

LRIT4AE (2012)

ICT in Low Resource Settings: Innovating for Africa and Europe through Living Labs

Read More  

ACRIPET (2008)

ACcurate Reconstruction In PET: Fully 3D PET reconstruction with compressed scatter system matrix

Read More