CARDIOBIOLIP

Novel mass spectrometry-based integrated analytical platforms for lipidomics studies on blood lipoproteins for cardiovascular disease research

Coordinatore	more  Organization address address: RAPENBURG 70 city: LEIDEN postcode: 2300 RA contact info Titolo: Mr. Nome: Ton Cognome: Brouwer Email: send email Telefono: +31 71 527 3149 Fax: +31 71 527 5269
Nazionalità Coordinatore	Non specificata
Totale costo	17˙597 €
EC contributo	0 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-05-15   -   2015-05-14

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITEIT LEIDEN  Organization address address: RAPENBURG 70 city: LEIDEN postcode: 2300 RA contact info Titolo: Mr. Nome: Ton Cognome: Brouwer Email: send email Telefono: +31 71 527 3149 Fax: +31 71 527 5269	NL (LEIDEN)	coordinator	175˙974.60

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 Obiettivo del progetto (Objective)

'Cardiovascular disease (CDV) is a serious health issue and one of the main causes of death in developed countries. The connection between lipids and the disease progress has been established in the past years. Particularly, lipid composition in blood circulating lipoproteins may have a significant correlation with CVD and CVD risk. However, although in the past years lipidomics-based platforms have been developed and mainly focused on the lipid profiling in blood in diverse studies, little attention has been drawn so far to their study according to lipoprotein fractions, which is essential. In this project, the development of an integrated analytical platform will be tackled for the separation of lipoproteins from plasma samples, extraction of lipids in the protein fractions and lipid profiling. Lipoprotein separations by capillary electrophoresis will be coupled on-line to liquid-liquid extraction of lipids and further LC-MS/MS, to constitute the first integrated platform for this type of analysis. The implementation of the platform in a lab-on-a-chip system will be explored for achieving fast, automated, low sample requirement, high-throughput analyses. This will open-up a wide range of analytical possibilities and new insights in the lipid biochemistry in cardiovascular disease. The methodology will be applied for the analysis of plasma blood samples from healthy volunteers and individuals with different degrees of CVD for disease biomarker discovery. Finally, the analysis of samples from tracer-based studies in dynamic systems in mouse models will also be possible. In addition, this will allow studying the effect on drugs on lipid biochemistry, and to fine-tune exciting therapies or to develop new treatment options in CVD.'