NANO-MRI

QUARTERNARY STRUCTURE IMAGING WITH NANO-MAGNETIC RESONANCE IMAGING (NANO-MRI)

Coordinatore	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH    more  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Christian Cognome: Degen Email: send email Telefono: +41 44 633 23 36
Nazionalità Coordinatore	Switzerland [CH]
Totale costo	184˙709 €
EC contributo	184˙709 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-IEF
Funding Scheme	MC-IEF
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-04-01   -   2015-03-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Christian Cognome: Degen Email: send email Telefono: +41 44 633 23 36	CH (ZUERICH)	coordinator	184˙709.40

Mappa

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 Obiettivo del progetto (Objective)

'There are three major problems in structure determination that can be circumvented using the proposed method. First, currently available methods such as nuclear magnetic resonance, X-ray crystallography and cryo electron microscopy require averaging over thousands of identical molecules at least. Second, X-ray crystallography and cryo electron microscopy lead to radiation damage of the sample. Third, NMR and X-ray crystallography are limited to a certain molecular size or shape. Magnetic Resonance Imaging (MRI) is an attractive alternative since it is non destructive, has elemental contrast and is possible for single particles. However, conventional MRI has only a few microns resolution. Magnetic resonance force microscopy, the method this proposal is based on, combines the principle of MRI with scanning force microscopy and thus pushes the limits of resolution down to the nanometer range. I will add domain contrast and push the limits of resolution further by introducing partial labeling. I will test and apply this entirely new concept to tobacco mosaic viruses. That will lead to a 3D protein structure with domain contrast with nanometer resolution from a single virus particle. This method is especially promising for large, rare or heterogeneous proteins that cannot be analyzed with state of the art methods.'

Introduzione (Teaser)

Structure is often the springboard from which functional studies are designed to solve complex problems in medicine and biology and thus provides invaluable information. Novel technology promises elemental resolution with a single sample.