PINP

Synthesis of peptide imprinted nanoparticles and their integration to ELISA-like assay for the quantification of hepcidin

Coordinatore	UNIVERSITA DEGLI STUDI DI VERONA    more  Organization address address: VIA DELL ARTIGLIERE 8 city: VERONA postcode: 37129 contact info Titolo: Dr. Nome: Maddalena Cognome: Manzini Email: send email Telefono: +39 045 8027957 Fax: +39 045 8027929
Nazionalità Coordinatore	Italy [IT]
Totale costo	179˙739 €
EC contributo	179˙739 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-IEF
Funding Scheme	MC-IEF
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-05-01   -   2015-04-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITA DEGLI STUDI DI VERONA  Organization address address: VIA DELL ARTIGLIERE 8 city: VERONA postcode: 37129 contact info Titolo: Dr. Nome: Maddalena Cognome: Manzini Email: send email Telefono: +39 045 8027957 Fax: +39 045 8027929	IT (VERONA)	coordinator	179˙739.60

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 Obiettivo del progetto (Objective)

'The development of assays based on molecular recognition for measuring biomarkers is a critical task for the diagnosis and prognosis of pathologies. The present project aims at developing synthetic nanoparticles (NP) for the quantification of the peptide biomarker hepcidin (hep) which regulates iron homeostasis. A modern strategies for mimicking receptors consists of NPs produced by molecularly imprinted polymer (MIP) technology. MIPs are prepared by a template-assisted emulsion polymerization. After removal of the template, complementary cavities are exposed in the polymer. The target biomarker, hep, is a 25-residue peptide (hep25) present in urine and plasma, other N-terminus truncated isoforms such as hep20 and hep22 were also found in humans. It is known that the first 5 amino acids of the N-terminus of hep25 are essential for iron homeostasis while the role of other isoforms is still unclear. Given that the imprinting is more successful for short peptides only 2 crucial portions of the full length peptide are synthesized as templates which are also functionalized to impose a direction of imprinting. The precision of the template synthesis ensures a higher fidelity of imprinting during the NPs synthesis improving the molecular recognition. The goal of the project consist in designing an assay for the determination of serum and/or urinary hep, based on MIPs selective for hep isoforms and arranged in a pseudo-immuno assay format, further extended in sensitivity by the addition of fluorescent tags. At last, an evaluation of the assay with clinical sample is planned to be performed. The possibility of using nano-sized MIPs as plastic antibodies in ELISA-like assays has the obvious advantage of a potential widespread diffusion. The proposed ELISA-like assay with fluorescent tags is very innovative and have the potential versatility to be transposed to different applications. Moreover the deep impact of the outcomes allow the access to the diagnostic market.'