safeDNA - self-assembled fluorescence enhancers for the Detection of Nucleic Acids


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 Nazionalità Coordinatore Germany [DE]
 Totale costo 167˙588 €
 EC contributo 149˙491 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2013-PoC
 Funding Scheme CSA-SA(POC)
 Anno di inizio 2014
 Periodo (anno-mese-giorno) 2014-02-01   -   2015-07-31


# participant  country  role  EC contrib. [€] 

 Organization address address: POCKELSSTRASSE 14
postcode: 38106

contact info
Titolo: Ms.
Nome: Kirsten-Illona
Cognome: Talk
Email: send email
Telefono: +49 531 391 5342
Fax: +49 0531 391 5334

DE (BRAUNSCHWEIG) hostInstitution 149˙491.00


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

nanosized    signal    readout    assembled    patent    self    hotspot    diagnostics    detection    fluorescence    nanolens    ct    direct    amplification    dna    enhancement    faster    simpler    scheme   

 Obiettivo del progetto (Objective)

'In this project we will prepare the commercialization of a new patent-protected signal amplification scheme for simpler and faster molecular diagnostics of pathogens such as Chlamydia trachomatis (CT). CT causes more cases of sexually transmitted diseases (STD) than any other bacterial pathogen, making CT infections an enormous public health problem throughout the world. Nowadays, diagnosis is commonly carried out using nucleic acid detection by fluorescence readout after polymerase chain reaction (PCR). However, DNA amplification requires technical equipment too sophisticated for application in developing countries or in standard labs in a medical practice. For cheaper, faster and even point-of-care diagnostics, a simpler amplification mechanism for a testing method capable of detecting DNA in small concentration is required.

Here, we suggest applying a new and universal signal amplifying approach that does not require any extra steps after target recognition. It relies on direct enhancement of the fluorescence readout signal using a self-assembled nanolens that was invented within the framework of the ERC starting grant SiMBA. The self-assembled nanolens is capable of enhancing the intensity of fluorescent dyes in a nanosized hotspot by more than two orders of magnitude. Further enhancement is conceivable. Braunschweig University of Technology has filed a broad patent application securing intellectual property rights for the project.

We will test this direct fluorescence enhancement scheme for the fast and sensitive detection of CT. Therefore, the self-assembled nanolens will be adapted for the detection of a CT target DNA by equipping the nanosized hotspot of the nanolens with probe DNA sequences. The target is then visualized by sandwich hybridization of a second specific, fluorescently labeled DNA strand. We envision an improved test for CT detection: it should be faster, simpler and price-competitive.'

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