SM-DNA-REPAIR

New single-molecule techniques and their application in the study of DNA break repair

 Coordinatore AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS 

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 Nazionalità Coordinatore Spain [ES]
 Totale costo 1˙624˙230 €
 EC contributo 1˙624˙230 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2007-StG
 Funding Scheme ERC-SG
 Anno di inizio 2008
 Periodo (anno-mese-giorno) 2008-08-01   -   2013-07-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    FUNDACIO INSTITUT CATALA DE NANOCIENCIA I NANOTECNOLOGIA

 Organization address address: CAMPUS DE LA UAB EDIFICI Q ICN2
city: BELLATERRA (BARCELONA)
postcode: 8193

contact info
Titolo: Dr.
Nome: Carlos Manuel
Cognome: Abad Ruiz
Email: send email
Telefono: +34 915681702
Fax: +34 915681702

ES (BELLATERRA (BARCELONA)) beneficiary 0.00
2    UNIVERSITY OF BRISTOL

 Organization address address: TYNDALL AVENUE SENATE HOUSE
city: BRISTOL
postcode: BS8 1TH

contact info
Titolo: Ms.
Nome: Johanna
Cognome: Rule
Email: send email
Telefono: +44 117 928 8696
Fax: +44 117 925 0900

UK (BRISTOL) beneficiary 0.00
3    AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

 Organization address address: CALLE SERRANO 117
city: MADRID
postcode: 28006

contact info
Titolo: Mr.
Nome: Alberto
Cognome: Sereno Alvarez
Email: send email
Telefono: +34 91 566 8852
Fax: +34 91 566 8913

ES (MADRID) hostInstitution 0.00
4    AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

 Organization address address: CALLE SERRANO 117
city: MADRID
postcode: 28006

contact info
Titolo: Prof.
Nome: Fernando
Cognome: Moreno Herrero
Email: send email
Telefono: +34 649893032
Fax: +34 935814747

ES (MADRID) hostInstitution 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

break    data    dna    fork    imaging    biophysical    collapse    cell    dynamics    recombination    molecule    mechanism    techniques    repair    conventional    replication    single    breaks   

 Obiettivo del progetto (Objective)

'Unrepaired DNA breaks can lead to genomic instability or cell death. They occur frequently during normal cellular metabolism and are caused, for example, by the collapse or stalling of the replication fork in response to DNA damage. Proper DNA-end processing and handling are essential for the survival of the cell and prevention of carcinogenesis. Cells possess robust mechanisms to repair DNA breaks. One such DNA repair mechanism is homologous recombination where the sister chromatid is used as a template for the faithful repair of the DNA break. In Bacteria, this pathway is initiated when a DNA end is processed to a 3ï‚¢-ssDNA overhang terminated at a recombination hotspot (Chi) sequence. This is a substrate for formation of a RecA nucleoprotein filament that catalyses strand exchange to promote repair. Recent data implicate the AddAB helicase-nuclease and the SMC (Structural Maintenance of Chromosomes) complex in the DNA break processing mechanism of the model organism Bacillus subtilis. Interaction between these machines provides a molecular link between DNA dynamics and the initiation of DNA break processing that may co-ordinate replication fork collapse and DNA repair. Single-molecule manipulation and imaging techniques offer huge potential to investigate DNA break repair reactions in completely new ways, providing information that is inaccessible to conventional ensemble experiments. The aim of this project is two-fold: firstly, to develop novel biophysical instruments for fast Atomic Force Microscopy imaging in liquid and a combined Optical and Magnetic Tweezers setup; and secondly, to monitor and characterize the real-time dynamics of these DNA-repair processes using these new and complementary biophysical approaches. Single-molecule investigation will be supported by statistical analysis of the data and conventional bulk biochemical techniques.'

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