XCIP

X-Chromosome Inactivation and Plasticity in pre-implantation mouse embryo

Coordinatore	INSTITUT PASTEUR    more  Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 contact info Titolo: Dr. Nome: Philip Cognome: Avner Email: send email Telefono: +33 1 45 68 86 25 Fax: +33 1 45 68 86 56
Nazionalità Coordinatore	France [FR]
Totale costo	30˙000 €
EC contributo	30˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-2-2-ERG
Funding Scheme	MC-ERG
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-03-01   -   2010-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT PASTEUR  Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 contact info Titolo: Dr. Nome: Philip Cognome: Avner Email: send email Telefono: +33 1 45 68 86 25 Fax: +33 1 45 68 86 56	FR (PARIS CEDEX 15)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'In mammals, one of the two X chromosomes is inactivated in females to enable dosage compensation for X-linked gene products. In mice, the first changes associated with X-inactivation occur during early pre-implantation embryogenesis. At the morula-blastocyst transition, paternal and maternal X chromosomes are differentially reprogrammed in the three cell lineages constitutive of the late blastocyst. In order to characterise the factors and epigenetic marks involved in this reprogramming process, we propose to compare the X chromatin structures in model cell lines derived from blastocysts where the paternal and maternal X chromosomes can be distinguished. To do this, we will analyse chromatin modifications and chromatin compaction using a polymorphic tiling array covering 3,5Mb around the X-inactivation centre – a genomic region on the X chromosome controlling initiation of X-inactivation. We will also study the nuclear organisation of the two X chromosomes using three-dimensional Fluorescence In Situ Hybridisation (3D FISH) techniques. These experiments will be repeated in pre-implantation embryos per se. This project addresses important early developmental decisions in mammalian embryology and should help to characterise the molecular principles involved in epigenetic reprogramming and genome plasticity during early embryogenesis.'