PATHO IRON

Cytosolic iron metabolism in unicellular eukaryotic pathogens

Coordinatore	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE    more  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Ms. Nome: Liliane Cognome: Flabbée Email: send email Telefono: +33 1 42 34 94 51 Fax: +33 1 43 26 87 23
Nazionalità Coordinatore	France [FR]
Totale costo	133˙662 €
EC contributo	133˙662 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-2-1-IEF
Funding Scheme	MC-IEF
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-08-04   -   2010-02-03

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Ms. Nome: Liliane Cognome: Flabbée Email: send email Telefono: +33 1 42 34 94 51 Fax: +33 1 43 26 87 23	FR (PARIS)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'Iron is an essential nutrient for virtually all living organisms and host iron availability plays a critical role in the host–pathogen relationship. Without effective mechanisms for acquisition, transport and utilization of iron, no organism especially parasites with high iron requirements, can survive. However, our knowledge about intracellular iron metabolism in eukaryotic pathogenic microorganisms is poor; it is not known, how iron is stored in the cell or how it is utilized. In the proposed project, we will investigate cytosolic iron metabolism in three unicellular eukaryotic pathogens: Candida albicans, Trichomonas vaginalis and Giardia intestinalis. We will use two different proteomic methods to identify proteins involved in iron metabolism: 1) 2D gel electrophoresis of cytosolic fractions from cells grown under iron-rich and iron-restricted conditions and 2) separation of 55Fe radiolabeled cytosolic iron complexes by liquid chromatography followed by native electrophoresis. Obtained proteins/protein complexes will be identified by Matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography-tandem mass spectrometry. We will test the cellular localization and the effect of the identified proteins on iron metabolism by overexpression in pathogens and yeast. The project has great potential to discover new mechanisms of iron metabolism as well as identify new therapeutic approaches for the treatment of human pathogens. This multidisciplinary project investigating different pathogenic organisms and using a broad range of state-of-the-art techniques and approaches will allow a young researcher with outstanding track record and potential to return to Europe, integrate into European scientific community, consolidate his research maturity and provide him with a strong background for establishing his own laboratory in later stages of his career.'

Introduzione (Teaser)

A European study investigated how unicellular microorganisms metabolise iron. Project results pave the way for the development of new approaches to treating human pathogens.