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AEGILWHEAT

Widening gene pool of bread wheat by hybridization with Aegilops biuncialis supported by advanced genetic and chromosome genomic approaches

Total Cost €

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EC-Contrib. €

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Partnership

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Outcomes and
results

AEGILWHEAT project word cloud

Explore the words cloud of the AEGILWHEAT project. It provides you a very rough idea of what is the project "AEGILWHEAT" about.

genus leaf pool ieb skills genomics flow bioinformatic agronomically marker traits relatives functional content cytomolecular linkage innovative gene sustain lack interspecific alleles aegilwheat map structure training extend genomes rust genomic olomouc grain cz resistance resolution adverse ordered speed sorting wild genes throughput hungary bread indels tolerance abiotic polymorphic tools he centre chromosomes triticum source date valuable expertise geneticists yield transfer breeders mitotic integrates evolution cytometric donors introgression molecular hybridization stress markers aegilops employing structural environmental genome breeding hampered assisted individual aestivum diversity biuncialis widening chromosome wheat tribe snps genetic plant lines nutritional species multidisciplinary generation isolate ae quality triticeae genetics quantity select sequencing bioinformatics

Project "AEGILWHEAT" data sheet

The following table provides information about the project.

Coordinator	USTAV EXPERIMENTALNI BOTANIKY AV CR Organization address address: ROZVOJOVA 263 city: PRAHA 6 postcode: 16502 website: www.ueb.cas.cz contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Czech Republic [CZ]
Project website	https://olomouc.ueb.cas.cz/en/research/projects/widening-gene-pool-of-bread-wheat-by-hybridization
Total cost	154˙720 €
EC max contribution	154˙720 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2016
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-09-01 to 2019-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	USTAV EXPERIMENTALNI BOTANIKY AV CR USTAV EXPERIMENTALNI BOTANIKY AV CR Organization address address: ROZVOJOVA 263 city: PRAHA 6 postcode: 16502 website: www.ueb.cas.cz contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	CZ (PRAHA 6)	coordinator	154˙720.00

Map

Project objective

The widening of gene pool of bread wheat (Triticum aestivum) by employing its wild relatives as donors of new alleles and genes will ensure the ability of wheat to sustain yield quality and quantity under adverse environmental conditions. Species from genus Aegilops represent a rich source of agronomically important traits, such as leaf rust resistance, grain nutritional quality and abiotic stress tolerance. To date, the utilization of wild genetic diversity has been hampered by low throughput of methods used to select introgression lines and the lack of knowledge on the genomes of wild relatives. The multidisciplinary AEGILWHEAT project integrates the applicant’s expertise in interspecific hybridization and cytomolecular genome analysis of Aegilops species with the state of art expertise in flow-cytometric sorting and genomic analysis of plant mitotic chromosomes in the Centre of Plant structural and Functional genomics (IEB, Olomouc, CZ). The aims of this project are to (1) isolate individual U and M chromosomes from Aegilops biuncialis and (2) identify their gene content and produce polymorphic markers (SNPs, INDELs) by next-generation sequencing and bioinformatic analyses. The genes and new markers will be integrated into (3) a new high resolution genetic linkage map of Ae. biuncialis. The marker-assisted selection system based on the ordered markers will be used for the (4) high throughput selection of wheat-Ae. biuncialis introgression lines. The training through the research will extend the expertise of the applicant by new skills in molecular genetics, genomics and bioinformatics so that he can apply innovative genomics approaches to speed up his wheat pre-breeding programmes in Hungary. The project will deliver valuable information to geneticists and breeders on chromosome structure and genome evolution within the tribe Triticeae and provide molecular tools to support cost-effective transfer of wild alleles into wheat.

Publications

List of publications.
year	authors and title	journal	last update
2019	Mahmoud Said, Alejandro Copete Parada, Eszter GaÃ¡l, IstvÃ¡n MolnÃ¡r, AdoraciÃ³n Cabrera, Jaroslav DoleÅ¾el, Jan VrÃ¡na Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers published pages: 2881-2898, ISSN: 0040-5752, DOI: 10.1007/s00122-019-03394-1	Theoretical and Applied Genetics 132/10	2020-01-16
2019	Mahmoud Said, Marie KubalÃ¡kovÃ¡, Miroslava KarafiÃ¡tovÃ¡, IstvÃ¡n MolnÃ¡r, Jaroslav DoleÅ¾el, Jan VrÃ¡na Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting published pages: , ISSN: 1940-3372, DOI: 10.3835/plantgenome2018.12.0096	The Plant Genome 12/2	2020-01-16
2019	Marianna Rakszegi, Ã‰va DarkÃ³, Alison Lovegrove, IstvÃ¡n MolnÃ¡r, LÃ¡szlÃ³ LÃ¡ng, ZoltÃ¡n BedÅ‘, MÃ¡rta MolnÃ¡r-LÃ¡ng, Peter Shewry Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines published pages: e0211892, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0211892	PLOS ONE 14/2	2020-01-16
2018	LÃ¡szlÃ³ Ivanizs, AndrÃ¡s Farkas, Gabriella Linc, MÃ¡rta MolnÃ¡r-LÃ¡ng, IstvÃ¡n MolnÃ¡r Molecular cytogenetic and morphological characterization of two wheat-barley translocation lines published pages: e0198758, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0198758	PLOS ONE 13/6	2020-01-16
2018	Edina TÃ¼rkÃ¶si, Eva Darko, Marianna Rakszegi, IstvÃ¡n MolnÃ¡r, MÃ¡rta MolnÃ¡r-LÃ¡ng, AndrÃ¡s Cseh Development of a new 7BS.7HL winter wheat-winter barley Robertsonian translocation line conferring increased salt tolerance and (1,3;1,4)-Î²-D-glucan content published pages: e0206248, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0206248	PLOS ONE 13/11	2020-01-16
2018	Eszter GaÃ¡l, Miroslav ValÃ¡rik, IstvÃ¡n MolnÃ¡r, AndrÃ¡s Farkas, Gabriella Linc Identification of COS markers specific for Thinopyrum elongatum chromosomes preliminary revealed high level of macrosyntenic relationship between the wheat and Th. elongatum genomes published pages: e0208840, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0208840	PLOS ONE 13/12	2020-01-16

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